Morroniside

Name: Morroniside
Brand: MedChemExpress
SKU: HY-N0532
Rating: 4.5 (1 reviews)

Cat. No.: HY-N0532 Purity: 99.88%: FAQs
Data Sheet SDS COA Handling Instructions

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Morroniside has neuroprotective effect by inhibiting neuron apoptosis and MMP2/9 expression.

For research use only. We do not sell to patients.

Morroniside Chemical Structure

CAS No. : 25406-64-8

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 66	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 66	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 28	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 90	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 190	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Morroniside:

Morroniside (Standard) Get quote

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•Related Screening Libraries:

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View All MMP Isoform Specific Products:

View All Isoforms

MMP-1 MMP-2 MMP-3 MMP-8 MMP-9 MMP-13 MMP-14 MMP-17 ADAM10 ADAM17 MMP-7 MMP-12 MMP-10 MMP ADAM8

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Morroniside has neuroprotective effect by inhibiting neuron apoptosis and MMP2/9 expression.

IC₅₀ & Target^[1]

MMP2

MMP9

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	IC₅₀	> 50 μM Compound: 17	Cytotoxicity against human HeLa cells after 48 hrs by MTT assay Cytotoxicity against human HeLa cells after 48 hrs by MTT assay	[PMID: 23002924]

In Vivo

Morroniside reduces the expression of MMP2 and MMP9 in an I/R injury model. Treatment with Morroniside significantly reduces I/R‑associated neuron apoptosis in a dose dependent manner. The results demonstrate that active caspase‑3 and Bax are significantly upregulated in the model group compared with the control group, while Bcl‑2 is significantly downregulated. The expression of active caspase‑3 and Bax is significantly downregulated by Morroniside treatment in a dose‑dependent manner, while the expression of Bcl‑2 is significantly upregulated^[1]. Morroniside has an ameliorative effect on diabetes-induced alterations such as oxidative stress, inflammation, and apoptosis in the liver of type 2 diabetic db/db mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

406.38

Formula

C₁₇H₂₆O₁₁

CAS No.

25406-64-8

Appearance

Solid

Color

White to off-white

SMILES

O[C@H]([C@@H](O)[C@@H]1O)[C@](O[C@@H]1CO)([H])O[C@H]2[C@@]([C@@H]3C)([H])[C@@](C[C@H](O)O3)([H])C(C(OC)=O)=CO2

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (246.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (123.04 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4608 mL	12.3038 mL	24.6075 mL
5 mM	0.4922 mL	2.4608 mL	4.9215 mL
10 mM	0.2461 mL	1.2304 mL	2.4608 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (246.08 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.88%

Select Batch:

Data Sheet (282 KB) SDS (393 KB)

COA (259 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Zeng G, et al. Morroniside protects against cerebral ischemia/reperfusion injury by inhibiting neuron apoptosis and MMP2/9 expression. Exp Ther Med. 2018 Sep;16(3):2229-2234. [Content Brief]

[2]. Park CH, et al. Evaluation of morroniside, iridoid glycoside from Corni Fructus, on diabetes-induced alterations such as oxidative stress, inflammation, and apoptosis in the liver of type 2 diabetic db/db mice. Biol Pharm Bull. 2011;34(10):1559-65. [Content Brief]

[3]. Huan Yu, et al. Morroniside attenuates apoptosis and pyroptosis of chondrocytes and ameliorates osteoarthritic development by inhibiting NF-κB signaling. J Ethnopharmacol. 2021 Feb 10;266:113447. [Content Brief]

Animal Administration
^[1]

Rats^[1]
A total of 50 adult male Sprague Dawley rats (age, 7 8 weeks; weight, 260 280 g) are used. Rats are randomly assigned into five groups (n=10 in each). Rats in the control group undergo sham surgery. All other rats undergo suture occluded surgery, with a 0.26 mm nylon monofilament inserted through the right common carotid artery and are divided into groups as follows: The cerebral I/R injury model group (model), no treatment; low dose group, 30 mg/kg/day Morroniside by gavage; moderate dose group, 90 mg/kg/day Morroniside by gavage; high dose group, 270 mg/kg/day Morroniside by gavage. Rats in the control and model groups receive an equal volume of normal saline^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Zeng G, et al. Morroniside protects against cerebral ischemia/reperfusion injury by inhibiting neuron apoptosis and MMP2/9 expression. Exp Ther Med. 2018 Sep;16(3):2229-2234. [Content Brief]

[2]. Park CH, et al. Evaluation of morroniside, iridoid glycoside from Corni Fructus, on diabetes-induced alterations such as oxidative stress, inflammation, and apoptosis in the liver of type 2 diabetic db/db mice. Biol Pharm Bull. 2011;34(10):1559-65. [Content Brief]

[3]. Huan Yu, et al. Morroniside attenuates apoptosis and pyroptosis of chondrocytes and ameliorates osteoarthritic development by inhibiting NF-κB signaling. J Ethnopharmacol. 2021 Feb 10;266:113447. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.4608 mL	12.3038 mL	24.6075 mL	61.5188 mL
	5 mM	0.4922 mL	2.4608 mL	4.9215 mL	12.3038 mL
	10 mM	0.2461 mL	1.2304 mL	2.4608 mL	6.1519 mL
	15 mM	0.1641 mL	0.8203 mL	1.6405 mL	4.1013 mL
	20 mM	0.1230 mL	0.6152 mL	1.2304 mL	3.0759 mL
	25 mM	0.0984 mL	0.4922 mL	0.9843 mL	2.4608 mL
	30 mM	0.0820 mL	0.4101 mL	0.8203 mL	2.0506 mL
	40 mM	0.0615 mL	0.3076 mL	0.6152 mL	1.5380 mL
	50 mM	0.0492 mL	0.2461 mL	0.4922 mL	1.2304 mL
	60 mM	0.0410 mL	0.2051 mL	0.4101 mL	1.0253 mL
	80 mM	0.0308 mL	0.1538 mL	0.3076 mL	0.7690 mL
	100 mM	0.0246 mL	0.1230 mL	0.2461 mL	0.6152 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Morroniside Related Classifications

Neurological Disease Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Morroniside25406-64-8MMPPyroptosisApoptosisMatrix metalloproteinasesInhibitorinhibitorinhibit

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Morroniside

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Other Forms of Morroniside:

Morroniside Related Antibodies

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•Related Screening Libraries:

•Related Small Molecules:

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View All MMP Isoform Specific Products:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

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Dilution Calculator

Complete Stock Solution Preparation Table

Morroniside Related Classifications

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