2. Anti-infection
  3. Bacterial Antibiotic Flavivirus
  4. Dianemycin

Dianemycin  (Synonyms: Nanchangmycin free acid)

Cat. No.: HY-100528A Purity: 99.67%
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Dianémycine (acide libre de nanchangmycine), un antibiotique polyéther produit par Streptomyces nanchangensis NS3226, inhibe les bactéries gram-positives. Nanchangmycine est un antiviral à large spectre actif contre le virus Zika.

Dianemycin (freie Säure von Nanchangmycin), ein Polyether-Antibiotikum, das durch Streptomyces nanchangensis NS3226 hergestellt wird, hemmt grampositive Bakterien. Nanchangmycin ist ein Breitspektrum-Antivirusmittel, das gegen das Zika-Virus wirkt.

Dianemycin (Nanchangmycin free acid), a polyether antibiotic produced by Streptomyces nanchangensis NS3226, inhibits gram-positive bacteria. Nanchangmycin is a broad spectrum antiviral active against Zika virus.

For research use only. We do not sell to patients.

Dianemycin Chemical Structure

Dianemycin Chemical Structure

CAS No. : 35865-33-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 191 In-stock
Solution
10 mM * 1 mL in DMSO USD 191 In-stock
Solid
1 mg USD 36 In-stock
5 mg USD 120 In-stock
10 mg USD 200 In-stock
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This product is a controlled substance and not for sale in your territory.

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Based on 2 publication(s) in Google Scholar

Other Forms of Dianemycin:

Dianemycin Related Antibodies

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Description

Dianemycin (Nanchangmycin free acid), a polyether antibiotic produced by Streptomyces nanchangensis NS3226, inhibits gram-positive bacteria[1]. Nanchangmycin is a broad spectrum antiviral active against Zika virus[2].

IC50 & Target

Bacteria[1]
Zika virus[2]
In Vitro

Dianemycin (Nanchangmycin) can be used as a growth promotant in poultry and to cure coccidiosis in chickens. Dianemycin is active against drug resistant strains of malaria[1].
Dianemycin (Nanchangmycin) as a potent inhibitor of Zika virus (ZIKV) entry across all cell types tested including physiologically relevant primary cells. Dianemycin potently reduces infection of all three strains of ZIKV across all three cell types. The IC50s for infection are between 0.1 and 0.4 μM while Dianemycin has low toxicity in these ranges. In addition, DENV is inhibited by Dianemycin across cell types[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Dianemycin Related Antibodies
Molecular Weight

867.11

Formula

C47H78O14
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@H]1[C@@]2(O[C@]([C@@]3([H])O[C@](O)([C@H](C)C[C@@H]3C)CO)([H])C[C@@H]2C)O[C@]([C@]4(O[C@]5(O[C@]([C@H](C)[C@@H](O)C5)([H])[C@@H](C)/C=C(C)/C([C@H](C)C[C@H](C)C(O)=O)=O)CC4)C)([H])C[C@@H]1O[C@@]6([H])O[C@@H]([C@@H](OC)CC6)C
Structure Classification
Initial Source

Streptomyces nanchangensis
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (115.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1533 mL 5.7663 mL 11.5326 mL
5 mM 0.2307 mL 1.1533 mL 2.3065 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.58 mg/mL (2.98 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.58 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.58 mg/mL (2.98 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.58 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.67%

COA (260 KB) HNMR (305 KB) RP-HPLC (247 KB) MS (69 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.1533 mL 5.7663 mL 11.5326 mL 28.8314 mL
5 mM 0.2307 mL 1.1533 mL 2.3065 mL 5.7663 mL
10 mM 0.1153 mL 0.5766 mL 1.1533 mL 2.8831 mL
15 mM 0.0769 mL 0.3844 mL 0.7688 mL 1.9221 mL
20 mM 0.0577 mL 0.2883 mL 0.5766 mL 1.4416 mL
25 mM 0.0461 mL 0.2307 mL 0.4613 mL 1.1533 mL
30 mM 0.0384 mL 0.1922 mL 0.3844 mL 0.9610 mL
40 mM 0.0288 mL 0.1442 mL 0.2883 mL 0.7208 mL
50 mM 0.0231 mL 0.1153 mL 0.2307 mL 0.5766 mL
60 mM 0.0192 mL 0.0961 mL 0.1922 mL 0.4805 mL
80 mM 0.0144 mL 0.0721 mL 0.1442 mL 0.3604 mL
100 mM 0.0115 mL 0.0577 mL 0.1153 mL 0.2883 mL
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Dianemycin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dianemycin35865-33-9NanchangmycinBacterialAntibioticFlavivirusInhibitorinhibitorinhibit

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