Penciclovir (Synonyms: BRL 39123; VSA 671)

Name: Penciclovir
Brand: MedChemExpress
SKU: HY-17424
Rating: 4.5 (1 reviews)

Cat. No.: HY-17424 Purity: 99.92%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Penciclovir (VSA 671) is a potent and selective anti-herpesvirus agent with EC₅₀ values of 0.5, 0.8 μg/ml for HSV-1 (HFEM), HSV-2 (MS), respectively. Penciclovir shows anti-herpesvirus activity with no-toxic. Penciclovir preventes mortality in mouse.

For research use only. We do not sell to patients.

Penciclovir Chemical Structure

CAS No. : 39809-25-1

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 28	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 28	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	USD 25	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 40	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Penciclovir:

1 Publications Citing Use of MCE Penciclovir

•Microchem J. 2021, 106587.

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All HSV Isoform Specific Products:

View All Isoforms

HSV-1 HSV-2 HSV

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

HSV-1

HSV-2

HSV-1

0.5 μg/mL (EC₅₀)

HSV-2

0.8 μg/mL (EC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
HEL	CC₅₀	> 100 μM Compound: 2	Cytotoxicity against human HEL cells assessed as cell growth inhibition by cell counting method Cytotoxicity against human HEL cells assessed as cell growth inhibition by cell counting method	[PMID: 28829913]
HEL	CC₅₀	> 395 μM Compound: Penciclovir	Cytotoxicity against HEL cells assessed as reduction in cell viability after 3 days by coulter counting method Cytotoxicity against HEL cells assessed as reduction in cell viability after 3 days by coulter counting method	[PMID: 29670705]
HepG2	EC₅₀	0.19 μM Compound: Penciclovir (PCV)	Effective concentration necessary to decrease extracellular Hepatitis B virus DNA levels by 50% in human hepatoblastoma cell line (2.2.15 cells) in vitro Effective concentration necessary to decrease extracellular Hepatitis B virus DNA levels by 50% in human hepatoblastoma cell line (2.2.15 cells) in vitro	10.1016/S0960-894X(97)00422-8
HepG2	IC₅₀	433 μM Compound: Penciclovir (PCV)	Cytotoxicity towards human hepatoblastoma cell line (2.2.15 cells) measured by inhibition of neutral red dye uptake in vitro Cytotoxicity towards human hepatoblastoma cell line (2.2.15 cells) measured by inhibition of neutral red dye uptake in vitro	10.1016/S0960-894X(97)00422-8
HepG2 2.2.15	EC₅₀	3.4 μM Compound: Penciclovir	Antiviral activity against Hepatitis B virus infected in human HepG2(2.2.15) cells assessed as reduction in viral DNA by Southern blotting Antiviral activity against Hepatitis B virus infected in human HepG2(2.2.15) cells assessed as reduction in viral DNA by Southern blotting	[PMID: 21333535]
HepG2 2.2.15	CC₅₀	552 μM Compound: Penciclovir	Cytotoxicity against human HepG2(2.2.15) cells by neutral red dye uptake assay Cytotoxicity against human HepG2(2.2.15) cells by neutral red dye uptake assay	[PMID: 21333535]
MRC5	IC₅₀	16 μM Compound: Penciclovir	Antiviral activity against Varicella zoster virus Ellen infected in human MRC5 cells assessed as inhibition of viral replication by viral reduction plaque assay Antiviral activity against Varicella zoster virus Ellen infected in human MRC5 cells assessed as inhibition of viral replication by viral reduction plaque assay	[PMID: 23219702]
MT4	IC₅₀	> 150 μM Compound: 2	Cytotoxicity against human MT4 cells assessed as reduction in cell viability by cell counting method Cytotoxicity against human MT4 cells assessed as reduction in cell viability by cell counting method	[PMID: 28829913]
Vero	CC₅₀	> 500 μM Compound: penciclovir	Compound was tested for cytotoxicity in Vero cells (concentration required to reduce the OD value by 50% of the control) Compound was tested for cytotoxicity in Vero cells (concentration required to reduce the OD value by 50% of the control)	[PMID: 9719596]
Vero	CC₅₀	> 790 μM Compound: PCV	Cytotoxicity against african green monkey Vero cells after 2 days Cytotoxicity against african green monkey Vero cells after 2 days	[PMID: 17438061]
Vero	EC₅₀	17 μM Compound: PCV	Antiviral activity against Herpes B virus 24105 infected in african green monkey Vero cells assessed as plaque reduction after 36 to 48 hrs Antiviral activity against Herpes B virus 24105 infected in african green monkey Vero cells assessed as plaque reduction after 36 to 48 hrs	[PMID: 17438061]
Vero	EC₅₀	17.3 μM Compound: PCV	Antiviral activity against Herpes B virus E90-136 infected in african green monkey Vero cells assessed as plaque reduction after 36 to 48 hrs Antiviral activity against Herpes B virus E90-136 infected in african green monkey Vero cells assessed as plaque reduction after 36 to 48 hrs	[PMID: 17438061]
Vero	EC₅₀	25.3 μM Compound: PCV	Antiviral activity against Herpes B virus 32425 infected in african green monkey Vero cells assessed as plaque reduction after 36 to 48 hrs Antiviral activity against Herpes B virus 32425 infected in african green monkey Vero cells assessed as plaque reduction after 36 to 48 hrs	[PMID: 17438061]
Vero	EC₅₀	4.7 μM Compound: PCV	Antiviral activity against Herpes simplex virus 1 F infected in african green monkey Vero cells assessed as plaque reduction after 36 to 48 hrs Antiviral activity against Herpes simplex virus 1 F infected in african green monkey Vero cells assessed as plaque reduction after 36 to 48 hrs	[PMID: 17438061]
Vero C1008	CC₅₀	> 400 μM Compound: Penciclovir	Cytotoxicity against African green monkey Vero E6 cells assessed as reduction of cell viability measured after 48 hrs by CCK-8 assay Cytotoxicity against African green monkey Vero E6 cells assessed as reduction of cell viability measured after 48 hrs by CCK-8 assay	[PMID: 32563814]
Vero C1008	CC₅₀	> 400 μM Compound: 54	Cytotoxicity against African green monkey Vero E6 cells by CCK-8 assay Cytotoxicity against African green monkey Vero E6 cells by CCK-8 assay	[PMID: 33486200]
Vero C1008	CC₅₀	> 400 μM Compound: 50	Cytotoxicity against African green monkey Vero E6 cells by the CCK8 assay Cytotoxicity against African green monkey Vero E6 cells by the CCK8 assay	[PMID: 32845145]
Vero C1008	CC₅₀	> 400 μM Compound: Penciclovir	Cytotoxicity against African green monkey Vero E6 cells by CCK8 assay Cytotoxicity against African green monkey Vero E6 cells by CCK8 assay	[PMID: 32511912]

In Vitro

Penciclovir (0-100 μg/ml) shows anti-herpesvirus activity with EC₅₀s of 0.5, 0.8, 2.4, 52, 100, 1.2, 1.6, 0.9, >100 μg/ml for HSV-1 (HFEM), HSV-2 (MS), VZV (Ellen), CMV (AD-169), BHV-1 (Oxford 1964), BHV-2 (New York 1), EHV-1(Quai Hals), FHV-1 (B927), SVV, respectively^[1].
Penciclovir (0-100 μg/ml) shows no-toxic to uninfected human cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:	MRC-5,WISH, Flow 4000, Flow 12000, RPMI 2650, WI-38, WI-38 VA13, Hs68, HEp-2, RD, SCC-13,Primary cells
Concentration:	0-100 µg/ml
Incubation Time:
Result:	Showed no-toxic with IC₅₀s of >100, >100, 100, >100, 60, >100, >100, >100, >100, >100, >100, >100 for MRC-5,WISH, Flow 4000, Flow 12000, RPMI 2650, WI-38, WI-38 VA13, Hs68, HEp-2, RD, SCC-13,Primary cells, respectively.

In Vivo

Penciclovir (100 mg/kg; s.c.; daily for 5 days) preventes mortality in mouse^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Three-week-old female Balb/c mice^[2]
Dosage:	100 mg/kg
Administration:	S.c.; daily for 5 days
Result:	Reduced virus titres in the respiratory organs of both w/t- and PR3-infected mice.

Clinical Trial

Molecular Weight

253.26

Formula

C₁₀H₁₅N₅O₃

CAS No.

39809-25-1

Appearance

Solid

Color

White to off-white

SMILES

O=C1NC(N)=NC2=C1N=CN2CCC(CO)CO

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (98.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 2 mg/mL (7.90 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.9485 mL	19.7426 mL	39.4851 mL
5 mM	0.7897 mL	3.9485 mL	7.8970 mL
10 mM	0.3949 mL	1.9743 mL	3.9485 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (249 KB) RP-HPLC (228 KB)

Handling Instructions (2659 KB)

References

[1]. M.R. Boyd, et al. Penciclovir: a review of its spectrum of activity, selectivity, and cross-resistance pattern. Antiviral Chemistry and Chemotherapy, 1993, (1): 3-11.

[2]. de la Fuente R, et al. The acyclic nucleoside analogue penciclovir is a potent inhibitor of equine herpesvirus type 1 (EHV-1) in tissue culture and in a murine model. Antiviral Res. 1992 May;18(1):77-89. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.9485 mL	19.7426 mL	39.4851 mL	98.7128 mL
H₂O / DMSO	5 mM	0.7897 mL	3.9485 mL	7.8970 mL	19.7426 mL
DMSO	10 mM	0.3949 mL	1.9743 mL	3.9485 mL	9.8713 mL
	15 mM	0.2632 mL	1.3162 mL	2.6323 mL	6.5809 mL
	20 mM	0.1974 mL	0.9871 mL	1.9743 mL	4.9356 mL
	25 mM	0.1579 mL	0.7897 mL	1.5794 mL	3.9485 mL
	30 mM	0.1316 mL	0.6581 mL	1.3162 mL	3.2904 mL
	40 mM	0.0987 mL	0.4936 mL	0.9871 mL	2.4678 mL
	50 mM	0.0790 mL	0.3949 mL	0.7897 mL	1.9743 mL
	60 mM	0.0658 mL	0.3290 mL	0.6581 mL	1.6452 mL
	80 mM	0.0494 mL	0.2468 mL	0.4936 mL	1.2339 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Penciclovir Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Penciclovir39809-25-1BRL 39123 VSA 671BRL39123BRL-39123VSA671VSA 671VSA-671HSVHerpes simplex virusanti-herpesvirusno-toxicmortalityInhibitorinhibitorinhibit

Please select a local distributor ナミキ商事株式会社コスモ・バイオ株式会社
Product Name			Salutation
Applicant Name *			Email Address *
Phone Number *			Organization Name *
Department *			Requested quantity *
Country or Region *
Remarks

Product Name			Lot #
Applicant Name *			Email Address *
Phone Number			Department *
Organization Name *			Country or Region *
Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Penciclovir (Synonyms: BRL 39123; VSA 671)

Customer Review

Other Forms of Penciclovir:

Penciclovir Related Antibodies

1 Publications Citing Use of MCE Penciclovir

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All HSV Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Penciclovir Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Penciclovir Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report