Pifithrin-β hydrobromide (Synonyms: PFT β hydrobromide; Cyclic Pifithrin-α hydrobromide)

Name: Pifithrin-β hydrobromide
Brand: MedChemExpress
SKU: HY-16702A
Rating: 5.0 (15 reviews)

Cat. No.: HY-16702A Purity: 99.97%: FAQs
Data Sheet SDS COA Handling Instructions

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Pifithrin-β hydrobromide (PFT β hydrobromide) is a potent p53 inhibitor with an IC₅₀ of 23 μM.

For research use only. We do not sell to patients.

Pifithrin-β hydrobromide Chemical Structure

CAS No. : 511296-88-1

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 80	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 80	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 73	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 124	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 248	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 405	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 630	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 15 publication(s) in Google Scholar

Other Forms of Pifithrin-β hydrobromide:

Pifithrin-β Get quote

14 Publications Citing Use of MCE Pifithrin-β hydrobromide

: Pifithrin-β hydrobromide purchased from MedChemExpress. Usage Cited in: PLoS One. 2018 Sep 20;13(9):e0203833. [Abstract]; Western Blot analysis of TFDP3, p53 and LC3 in whole cell lysates of MDA-MB-231 transfected and treated with indicated plasmids and reagents. Cells in the treatment group are incubated with 20 μM PFT-beta for 24 hours.

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Description

Pifithrin-β hydrobromide (PFT β hydrobromide) is a potent p53 inhibitor with an IC₅₀ of 23 μM.

IC₅₀ & Target

IC50: 23 μM (p53)^[1]

In Vitro

Pifithrin-α hydrobromide (PFT β hydrobromide), an inhibitor of the p53 protein, is regarded as a lead compound for cancer and neurodegenerative disease therapy. Pifithrin-α is very unstable in culture medium and rapidly converts to its condensation product pifithrin-β, the N-acetyl derivative^[2]. After 24 h, the viability assay shows that the pretreatments with 1 and 10 μM pifithrin-β exerts neuroprotective effects^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

349.29

Formula

C₁₆H₁₇BrN₂S

CAS No.

511296-88-1

Appearance

Solid

Color

White to off-white

SMILES

CC1=CC=C(C2=CN3C(SC4=C3CCCC4)=N2)C=C1.Br

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 10 mg/mL (28.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8630 mL	14.3148 mL	28.6295 mL
5 mM	0.5726 mL	2.8630 mL	5.7259 mL
10 mM	0.2863 mL	1.4315 mL	2.8630 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (2.86 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1 mg/mL (2.86 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1 mg/mL (2.86 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 10 mg/mL (28.63 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.97%

Select Batch:

Data Sheet (276 KB) SDS (252 KB)

COA (251 KB) LCMS (262 KB)

Handling Instructions (2659 KB)

References

[1]. Christodoulou MS, et al. Synthesis and biological evaluation of imidazolo[2,1-b]benzothiazole derivatives, as potential p53 inhibitors. Bioorg Med Chem. 2011 Mar 1;19(5):1649-57. [Content Brief]

[2]. Fernández-Cruz ML, et al. Biological and chemical studies on aryl hydrocarbon receptor induction by the p53 inhibitor pifithrin-α and its condensation productpifithrin-β. Life Sci. 2011 Apr 25;88(17-18):774-83. [Content Brief]

[3]. Da Pozzo E, et al. p53 functional inhibitors behaving like pifithrin-β counteract the Alzheimer peptide non-β-amyloid component effects in human SH-SY5Y cells. ACS Chem Neurosci. 2014 May 21;5(5):390-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8630 mL	14.3148 mL	28.6295 mL	71.5738 mL
	5 mM	0.5726 mL	2.8630 mL	5.7259 mL	14.3148 mL
	10 mM	0.2863 mL	1.4315 mL	2.8630 mL	7.1574 mL
	15 mM	0.1909 mL	0.9543 mL	1.9086 mL	4.7716 mL
	20 mM	0.1431 mL	0.7157 mL	1.4315 mL	3.5787 mL
	25 mM	0.1145 mL	0.5726 mL	1.1452 mL	2.8630 mL

Pifithrin-β hydrobromide Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pifithrin-β511296-88-1PFT βCyclic Pifithrin-αMDM-2/p53FerroptosisInhibitorinhibitorinhibit

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Pifithrin-β hydrobromide (Synonyms: PFT β hydrobromide; Cyclic Pifithrin-α hydrobromide)

Customer Review

Other Forms of Pifithrin-β hydrobromide:

Pifithrin-β hydrobromide Related Antibodies

14 Publications Citing Use of MCE Pifithrin-β hydrobromide

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Pifithrin-β hydrobromide Related Antibodies

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Complete Stock Solution Preparation Table

Pifithrin-β hydrobromide Related Classifications

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