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  3. Parasite
  4. Piperonyl butoxide

Piperonyl butoxide  (Synonyms: ENT-14250)

Cat. No.: HY-B1198 Purity: ≥98.0%
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Piperonyl butoxide (ENT-14250) is a pesticide synergist and food additive. Piperonyl butoxide has adverse effects on reproduction, development and behavior in mice. Piperonyl butoxide can activate c-Jun and ATF-2 in mouse hepatocytes. Piperonyl butoxide is a liver cancer carcinogen in rats and mice.

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Piperonyl butoxide Chemical Structure

Piperonyl butoxide Chemical Structure

CAS No. : 51-03-6

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10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

Other Forms of Piperonyl butoxide:

Piperonyl butoxide Related Antibodies

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Description

Piperonyl butoxide (ENT-14250) is a pesticide synergist and food additive. Piperonyl butoxide has adverse effects on reproduction, development and behavior in mice. Piperonyl butoxide can activate c-Jun and ATF-2 in mouse hepatocytes. Piperonyl butoxide is a liver cancer carcinogen in rats and mice[1][2][3].

In Vitro

Piperonyl butoxide (25 μg/mL; 24 h) induces phosphorylation of ATF-2 in mouse hepatocytes [1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Piperonyl butoxide Related Antibodies

Western Blot Analysis[1]

Cell Line: Mouse immortalized hepatocyte cell line TLR-3
Concentration: 25 μg/mL
Incubation Time: 24 h
Result: Significantly increased the phosphorylation level of ATF-2.
In Vivo

Piperonyl butoxide (0.6% in diet; Feed administration; 48 h) can activate c-Jun and ATF-2 in mouse hepatocytes, which may promote cell proliferation in the early stage of mouse liver cancer[1].
Piperonyl butoxide (0.025-0.4% in diet; Feed administration; 8 weeks) can adversely affect the exploratory behavior of female mice[2]
Pharmacokinetic Analysis in female C57BL/6J mice[4]

Route Dose CL (mL/h) V (mL) Fgavage Fchow IIV CL IIV V IIV Fgavage IIV Fchow Proportional Residual Error
i.g. 22.22 mg/kg 83.3 791 1 FIXED / 106.8 96.7 0 / 58.0
Daily diet 0.09% diet 83.3 FIXED 791 FIXED 1 FIXED 0.409 0 0 145 103 82.6

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male ICR mice aged 5 weeks old[1]
Dosage: Diet containing 0.6%
Administration: Feed administration; 48 h
Result: Elevated the level of liver nuclear proteins that bind to an AP-1 consensus oligonucleotide at 24 h of administration.
Induced c-Jun phosphorylation within 8 h of administration, and phosphorylated ATF-2 was detected after 24 h of administration.
Animal Model: Female mice (Crl: CD1) aged 5 weeks old[2]
Dosage: Diet containing 0.025%, 0.1%, and 0.4%
Administration: Feed administration; from 5 to 12 weeks of age.
Result: Had no significant effect on the average body weight of female mice through the experimental periods.
Had no significant effect on the performance of multiple-T water maze at 10 weeks of age.
Reduced the average speed in a dose-dependent manner in exploratory behaviour examination at 8 weeks of age.
Shortened the total exploration distance in a dose-dependent manner and decreased the average speed in exploratory behavior examination at 11 weeks of age.
Clinical Trial
Molecular Weight

338.44

Formula

C19H30O5
CAS No.
Appearance

Oil

Color

Colorless to light yellow

SMILES

CCCC1=C(COCCOCCOCCCC)C=C2OCOC2=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (295.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 100 mg/mL (295.47 mM; Need ultrasonic)

H2O : 0.67 mg/mL (1.98 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9547 mL 14.7737 mL 29.5473 mL
5 mM 0.5909 mL 2.9547 mL 5.9095 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.39 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (7.39 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO / Ethanol 1 mM 2.9547 mL 14.7737 mL 29.5473 mL 73.8683 mL
DMSO / Ethanol 5 mM 0.5909 mL 2.9547 mL 5.9095 mL 14.7737 mL
10 mM 0.2955 mL 1.4774 mL 2.9547 mL 7.3868 mL
15 mM 0.1970 mL 0.9849 mL 1.9698 mL 4.9246 mL
20 mM 0.1477 mL 0.7387 mL 1.4774 mL 3.6934 mL
25 mM 0.1182 mL 0.5909 mL 1.1819 mL 2.9547 mL
30 mM 0.0985 mL 0.4925 mL 0.9849 mL 2.4623 mL
40 mM 0.0739 mL 0.3693 mL 0.7387 mL 1.8467 mL
50 mM 0.0591 mL 0.2955 mL 0.5909 mL 1.4774 mL
60 mM 0.0492 mL 0.2462 mL 0.4925 mL 1.2311 mL
80 mM 0.0369 mL 0.1847 mL 0.3693 mL 0.9234 mL
100 mM 0.0295 mL 0.1477 mL 0.2955 mL 0.7387 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Piperonyl butoxide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Piperonyl butoxide51-03-6ENT-14250ENT14250ENT 14250ParasitePesticide inhibitorFood additiveHepatocyteLiver cancer carcinogenInhibitorinhibitorinhibit

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