RP 70676

Cat. No.: HY-101576 Purity: 99.73%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

RP 70676 is a potent inhibitor of ACAT, with IC₅₀ of 25 and 44 nM for rat and rabbit ACAT.

For research use only. We do not sell to patients.

RP 70676 Chemical Structure

CAS No. : 136609-26-2

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 308	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 308	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 280	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 420	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 756	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1060	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1484	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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ACAT1 ACAT2 MGAT2 ACAT

Biological Activity
Purity & Documentation
References
Customer Review

Description

RP 70676 is a potent inhibitor of ACAT, with IC₅₀ of 25 and 44 nM for rat and rabbit ACAT.

IC₅₀ & Target

IC50: 25 nM (Rat ACAT), 44 nM (Rabbit ACAT)^[1]

In Vitro

RP 70676 is a potent inhibitor of rabbit arterial ACAT (IC₅₀ = 40 nM) and has been shown to be an effective inhibitor of ACAT derived from a number of tissues and species including man. The IC₅₀ values range from 21 nM for hamster liver ACAT to 108 nM for enzyme from the intestine of cholesterol fed rabbits; in human hepatic tissues the mean IC₅₀ is 44 nM. In whole cell P388D, murine macrophages the compound has an IC₅₀ of 540 nM.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

RP 70676 (10 mg/kg, p.o.) is well absorbed with plasma levels in NZW rabbits^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

416.58

Formula

C₂₅H₂₈N₄S

CAS No.

136609-26-2

Appearance

Solid

Color

Off-white to brown

SMILES

CC1=CC(C)=NN1CCCCCSC2=NC(C3=CC=CC=C3)=C(C4=CC=CC=C4)N2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (300.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4005 mL	12.0025 mL	24.0050 mL
5 mM	0.4801 mL	2.4005 mL	4.8010 mL
10 mM	0.2400 mL	1.2002 mL	2.4005 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (4.99 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.74%

Select Batch:

Data Sheet (272 KB) SDS (252 KB)

COA (248 KB) HNMR (264 KB) LCMS (230 KB)

Handling Instructions (2659 KB)

References

[1]. M.J. Ashton, et al. RP 70676: A potent systematically available inhibitor of acyl-CoA:cholesterol O-acyl transferase (ACAT). Bioorganic & Medicinal Chemistry Letters Volume 2, Issue 5, 1992, Pages 375-380.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4005 mL	12.0025 mL	24.0050 mL	60.0125 mL
	5 mM	0.4801 mL	2.4005 mL	4.8010 mL	12.0025 mL
	10 mM	0.2400 mL	1.2002 mL	2.4005 mL	6.0012 mL
	15 mM	0.1600 mL	0.8002 mL	1.6003 mL	4.0008 mL
	20 mM	0.1200 mL	0.6001 mL	1.2002 mL	3.0006 mL
	25 mM	0.0960 mL	0.4801 mL	0.9602 mL	2.4005 mL
	30 mM	0.0800 mL	0.4001 mL	0.8002 mL	2.0004 mL
	40 mM	0.0600 mL	0.3001 mL	0.6001 mL	1.5003 mL
	50 mM	0.0480 mL	0.2400 mL	0.4801 mL	1.2002 mL
	60 mM	0.0400 mL	0.2000 mL	0.4001 mL	1.0002 mL
	80 mM	0.0300 mL	0.1500 mL	0.3001 mL	0.7502 mL
	100 mM	0.0240 mL	0.1200 mL	0.2400 mL	0.6001 mL

RP 70676 Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RP 70676136609-26-2RP70676RP-70676AcyltransferaseDiacylglycerol acyltransferaseDiglyceride acyltransferaseacyl-CoA:cholesterol acyltransferasemono- acylglycerol acyltransferaseInhibitorinhibitorinhibit

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