RO-28-1675

Name: RO-28-1675
Brand: MedChemExpress
SKU: HY-10595
Rating: 5.0 (2 reviews)

Cat. No.: HY-10595 Purity: 99.95%: FAQs
Data Sheet SDS COA Handling Instructions

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RO-28-1675 is a potent allosteric glucokinase (GK) activator with an EC₅₀ of 54 nM. RO-28-1675 can be used for the research of type 2 diabetes.

For research use only. We do not sell to patients.

RO-28-1675 Chemical Structure

CAS No. : 300353-13-3

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 284	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 284	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 258	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 444	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE RO-28-1675

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Purity & Documentation
References
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Description

RO-28-1675 is a potent allosteric glucokinase (GK) activator with an EC₅₀ of 54 nM. RO-28-1675 can be used for the research of type 2 diabetes^[1]^[2].

IC₅₀ & Target

EC50: 54 nM (glucokinase)^[1]

In Vitro

RO-28-1675 can reverse the inhibitory action of the human glucokinase regulatory protein (GKRP)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

RO-28-1675 (50 mg/kg; p.o.) reduces blood glucose levels in wild-type C57BL/6J mice^[2].
RO-28-1675 exhibits high oral bioavailability (mice 92.8%) and C_max (1140 μg/mL) following oral administration (10 mg/kg)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	10 weeks old male C57BL/6J mice^[2]
Dosage:	50 mg/kg
Administration:	Oral administration
Result:	Reduced blood glucose levels.

Animal Model:	C57BL/6J mice^[1]
Dosage:	10 mg/kg (Pharmacokinetic Analysis
Administration:	Oral administration
Result:	Oral bioavailability (92.8%), C_max (1140 μg/mL), T _max (3.3 h).

Molecular Weight

378.51

Formula

C₁₈H₂₂N₂O₃S₂

CAS No.

300353-13-3

Appearance

Solid

Color

White to off-white

SMILES

O=C([C@@H](C1=CC=C(C=C1)S(=O)(C)=O)CC2CCCC2)NC3=NC=CS3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (132.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6419 mL	13.2097 mL	26.4194 mL
5 mM	0.5284 mL	2.6419 mL	5.2839 mL
10 mM	0.2642 mL	1.3210 mL	2.6419 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.60 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.60 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.60 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.95%

Select Batch:

Data Sheet (280 KB) SDS (251 KB)

COA (255 KB) LCMS (134 KB)

Handling Instructions (2659 KB)

References

[1]. Nancy-Ellen Haynes, et al. Discovery, structure-activity relationships, pharmacokinetics, and efficacy of glucokinase activator (2R)-3-cyclopentyl-2-(4-methanesulfonylphenyl)-N-thiazol-2-yl-propionamide (RO0281675). J Med Chem. 2010 May 13;53(9):3618-25. [Content Brief]

[2]. Joseph Grimsby, et al. Allosteric activators of glucokinase: potential role in diabetes therapy. Science. 2003 Jul 18;301(5631):370-3. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6419 mL	13.2097 mL	26.4194 mL	66.0485 mL
	5 mM	0.5284 mL	2.6419 mL	5.2839 mL	13.2097 mL
	10 mM	0.2642 mL	1.3210 mL	2.6419 mL	6.6048 mL
	15 mM	0.1761 mL	0.8806 mL	1.7613 mL	4.4032 mL
	20 mM	0.1321 mL	0.6605 mL	1.3210 mL	3.3024 mL
	25 mM	0.1057 mL	0.5284 mL	1.0568 mL	2.6419 mL
	30 mM	0.0881 mL	0.4403 mL	0.8806 mL	2.2016 mL
	40 mM	0.0660 mL	0.3302 mL	0.6605 mL	1.6512 mL
	50 mM	0.0528 mL	0.2642 mL	0.5284 mL	1.3210 mL
	60 mM	0.0440 mL	0.2202 mL	0.4403 mL	1.1008 mL
	80 mM	0.0330 mL	0.1651 mL	0.3302 mL	0.8256 mL
	100 mM	0.0264 mL	0.1321 mL	0.2642 mL	0.6605 mL

RO-28-1675 Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RO-28-1675300353-13-3GlucokinaseHexokinase IVHexokinase Dglucokinasetype2diabetesregulatoryproteinGKdirectactivatorsGKAsInhibitorinhibitorinhibit

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RO-28-1675

Customer Review

RO-28-1675 Related Antibodies

2 Publications Citing Use of MCE RO-28-1675

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

RO-28-1675 Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

RO-28-1675 Related Classifications

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