2. Autophagy Neuronal Signaling PI3K/Akt/mTOR Apoptosis Metabolic Enzyme/Protease
  3. PINK1/Parkin mTOR Apoptosis Prolyl Endopeptidase (PREP)
  4. Salidroside

Salidroside  (Synonyms: Rhodioloside)

Cat. No.: HY-N0109 Purity: 99.90%
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Salidroside (Rhodioloside) is a prolyl endopeptidase inhibitor. Salidroside alleviates cachexia symptoms in mouse models of cancer cachexia via activating mTOR signalling. Salidroside protects dopaminergic neurons by enhancing PINK1/Parkin-mediated mitophagy.

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Salidroside Chemical Structure

Salidroside Chemical Structure

CAS No. : 10338-51-9

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Based on 25 publication(s) in Google Scholar

Other Forms of Salidroside:

Salidroside Related Antibodies

Top Publications Citing Use of Products

    Salidroside purchased from MedChemExpress. Usage Cited in: Int J Oncol. 2019 Jun;54(6):1969-1980.  [Abstract]

    Apoptosis‑related protein expression in MG63 cells in response to various concentrations of Salidroside is detected via western blot analysis.

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    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

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    Description

    Salidroside (Rhodioloside) is a prolyl endopeptidase inhibitor. Salidroside alleviates cachexia symptoms in mouse models of cancer cachexia via activating mTOR signalling. Salidroside protects dopaminergic neurons by enhancing PINK1/Parkin-mediated mitophagy.

    IC50 & Target[2]

    mTOR

     

    Cellular Effect
    Cell Line Type Value Description References
    HeLa IC50
    10.493 μM
    Compound: Sal
    Antiproliferative activity against human HeLa cells assessed as inhibition of cell proliferation measured after 48 hrs by CCK-8 assay
    Antiproliferative activity against human HeLa cells assessed as inhibition of cell proliferation measured after 48 hrs by CCK-8 assay
    		[PMID: 34978808]
    MDA-MB-231 IC50
    40 μM
    Compound: 2
    Anticancer activity against human MDA-MB-231 cells assessed as cell growth inhibition incubated for 24 hrs by MTT assay
    Anticancer activity against human MDA-MB-231 cells assessed as cell growth inhibition incubated for 24 hrs by MTT assay
    		[PMID: 33650861]
    PC-12 EC50
    0.3 μM
    Compound: Cpd i
    Neuroprotective activity against CoCl2-induced cytotoxicity in rat PC12 cells assessed as increase in cell viability pretreated with CoCl2 for 8 hrs followed by incubation with drug and measured after 24 hrs by MTT assay
    Neuroprotective activity against CoCl2-induced cytotoxicity in rat PC12 cells assessed as increase in cell viability pretreated with CoCl2 for 8 hrs followed by incubation with drug and measured after 24 hrs by MTT assay
    		[PMID: 33097301]
    In Vitro

    Salidroside (100 μM) inhibits prolyl endopeptidase (PEP) activity (10.6±1.9%). Prolyl endopeptidase is an enzyme that plays a role in the metabolism of proline-containing neuropeptidase which is recognized to be involved in learning and memory[1].
    Salidroside, one of the major phenylpropanoid glycosides found in R.?rosea L, is consumed almost daily as a nutritional supplement in many countries and has been identified possessing potential anti-fatigue and anoxia,anti-aging, and anti-Alzheimer's disease activities. Salidroside can improve muscle nutrition via increasing mTOR, p-mTOR, and MyHC expression[2].
    SH-SY5Y cells are exposed to 0-600?μM MPP+ for 12-48?h and the results show that MPP+ results in a significant decrease of cell viability in a concentration and time-dependent manner. Cells are pretreated with 25-100?μM Salidroside (Sal) for 24?h and then exposed to 500?μM MPP+ for an additional 24?h. Salidroside concentration-dependently prevents MPP+-induced decrease of cell viability. Annexin V/PI staining is a common method for the detection of apoptotic cell. Salidroside significantly decreases the number of Annexin V/PI-stained cells treated by MPP+ which is in a concentration-dependent manner. Apoptotic cell could also be morphologically evaluated by Hoechst staining. In Hoechst staining, apoptotic cells are characterized by reduced nuclear size, chromatin condensation, intense fluorescence, and nuclear fragmentation. Salidroside notably inhibits MPP+-induced increase of chromatin condensation, intense fluorescence, and nuclear fragmentation in SH-SY5Y cells[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Salidroside Related Antibodies
    In Vivo

    Salidroside is a natural antioxidant extracted from medicinal food plant Rhodiola rosea. Salidroside (100 mg/kg/day) shows strong glucose lowering effect on db/db mice which is similar to effect of Metformin (200 mg/kg/day)[4].
    Salidroside can be used to induce testicular injury model in mice[6].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    300.30

    Formula

    C14H20O7
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O[C@H]([C@H]([C@@H]([C@@H](CO)O1)O)O)[C@@H]1OCCC2=CC=C(O)C=C2
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    H2O : 125 mg/mL (416.25 mM; Need ultrasonic)

    DMSO : 100 mg/mL (333.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.3300 mL 16.6500 mL 33.3000 mL
    5 mM 0.6660 mL 3.3300 mL 6.6600 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (333.00 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    Dosing volume
    (per animal)

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.90%

    SDS (393 KB)

    COA (246 KB) HNMR (288 KB) LCMS (87 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [3]

    SH-SY5Y cells are seeded in 96-well plates at 1×104 cells per well. After the treatment with Salidroside (25-100?μM) and MPP+, cell viability is measured by MTT assay. Briefly, cells are incubated with 500?μg/mL MTT at 37°C for 4?h. After that, the medium is removed and 150?μL DMSO is added and shaking is conducted for 10?min. Absorbance is measured at 570?nm in a microplate reader and the results are expressed as folds of control[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [4]

    Mice[4]
    The 4-week-old male C57BL/6 mice are fed a high-fat diet (HFD) (n=16) or normal chow diet (n=8). After 10 weeks of the HFD, salidroside intervention (100 mg/kg/day) is initiated by gavage once a day for 5 weeks. The control groups are given vehicle (saline). The 4-week-old male C57Bl/KsJ (BKS) mice (wild type, n=8) and BKS.Cg-Dock7m +/+ Leprdb/J (db/db) mice (n=16) are used. Salidroside (100 mg/kg/day) is administrated orally by gavage once a day for 5 weeks. The control groups are given vehicle (saline). Fasting blood glucose and body weight of mice are monitored every 5 days. Glucose measurements are performed on blood drawn from the tail vein using a Glucometer.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 3.3300 mL 16.6500 mL 33.3000 mL 83.2501 mL
    5 mM 0.6660 mL 3.3300 mL 6.6600 mL 16.6500 mL
    10 mM 0.3330 mL 1.6650 mL 3.3300 mL 8.3250 mL
    15 mM 0.2220 mL 1.1100 mL 2.2200 mL 5.5500 mL
    20 mM 0.1665 mL 0.8325 mL 1.6650 mL 4.1625 mL
    25 mM 0.1332 mL 0.6660 mL 1.3320 mL 3.3300 mL
    30 mM 0.1110 mL 0.5550 mL 1.1100 mL 2.7750 mL
    40 mM 0.0833 mL 0.4163 mL 0.8325 mL 2.0813 mL
    50 mM 0.0666 mL 0.3330 mL 0.6660 mL 1.6650 mL
    60 mM 0.0555 mL 0.2775 mL 0.5550 mL 1.3875 mL
    80 mM 0.0416 mL 0.2081 mL 0.4163 mL 1.0406 mL
    100 mM 0.0333 mL 0.1665 mL 0.3330 mL 0.8325 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Salidroside Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Salidroside10338-51-9RhodiolosidePINK1/ParkinmTORApoptosisProlyl Endopeptidase (PREP)Mammalian target of Rapamycinprolyl oligopeptidasePOPInhibitorinhibitorinhibit

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