Schisantherin A (Synonyms: Gomisin-C; Schizantherin-A; Wuweizi ester-A)

Name: Schisantherin A
Brand: MedChemExpress
SKU: HY-N0694
Rating: 5.0 (2 reviews)

Cat. No.: HY-N0694 Purity: 99.87%: FAQs
Data Sheet SDS COA Handling Instructions

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Schisantherin A is a dibenzocyclooctadiene lignan. Schisantherin A inhibits p65-NF-κB translocation into the nucleus by IκBα degradation.

For research use only. We do not sell to patients.

Schisantherin A Chemical Structure

CAS No. : 58546-56-8

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 65	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 65	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 30	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 59	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 198	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 321	In-stock	Estimated Time of Arrival: December 31
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Based on 2 publication(s) in Google Scholar

Other Forms of Schisantherin A:

Schisantherin A (Standard) Get quote

1 Publications Citing Use of MCE Schisantherin A

•Life Sci. 2020 Oct 1;258:118161. [Abstract]

: Schisantherin A purchased from MedChemExpress. Usage Cited in: Life Sci. 2020 Oct 1;258:118161. [Abstract]; Western blot analysis shows a significant activation of NF-κB signaling, whereas pre-incubation of SchA (50 μM) could diminish LPS-triggered P65NF-κB phosphorylation. The dose-dependent experiment shows SchA at 25 μM or 50 μM could inhibit the activation of P65NF-κB after LPS stimulation for 3 hours.

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Schisantherin A is a dibenzocyclooctadiene lignan. Schisantherin A inhibits p65-NF-κB translocation into the nucleus by IκBα degradation.

IC₅₀ & Target^[1]

p65

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 100 μM Compound: Gomisin C	Antiproliferative activity against human A549 cells after 72 hrs by SRB assay Antiproliferative activity against human A549 cells after 72 hrs by SRB assay	[PMID: 18063366]
A549	GI₅₀	74.9 μM Compound: 1	Growth inhibition of human A549 cells after 48 hrs by SRB assay Growth inhibition of human A549 cells after 48 hrs by SRB assay	10.1039/C6MD00097E
DU-145	GI₅₀	13.3 μM Compound: 1	Growth inhibition of human DU145 cells after 48 hrs by SRB assay Growth inhibition of human DU145 cells after 48 hrs by SRB assay	10.1039/C6MD00097E
HCT-15	IC₅₀	> 100 μM Compound: Gomisin C	Antiproliferative activity against human HCT15 cells after 72 hrs by SRB assay Antiproliferative activity against human HCT15 cells after 72 hrs by SRB assay	[PMID: 18063366]
IMR-32	GI₅₀	3.6 μM Compound: 1	Growth inhibition of human IMR32 cells after 48 hrs by SRB assay Growth inhibition of human IMR32 cells after 48 hrs by SRB assay	10.1039/C6MD00097E
K562	IC₅₀	> 100 μM Compound: Gomisin C	Antiproliferative activity against human K562 cells after 72 hrs by MTT assay Antiproliferative activity against human K562 cells after 72 hrs by MTT assay	[PMID: 18063366]
MDA-MB-231	GI₅₀	2.8 μM Compound: 1	Growth inhibition of human MDA-MB-231 cells after 48 hrs by SRB assay Growth inhibition of human MDA-MB-231 cells after 48 hrs by SRB assay	10.1039/C6MD00097E
MDA-MB-231	IC₅₀	56.4 μM Compound: Gomisin C	Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by SRB assay Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by SRB assay	[PMID: 18063366]
PANC-1	GI₅₀	> 100 μM Compound: 1	Growth inhibition of human PANC1 cells after 48 hrs by SRB assay Growth inhibition of human PANC1 cells after 48 hrs by SRB assay	10.1039/C6MD00097E
SiHa	GI₅₀	14.7 μM Compound: 1	Growth inhibition of human SiHa cells after 48 hrs by SRB assay Growth inhibition of human SiHa cells after 48 hrs by SRB assay	10.1039/C6MD00097E
SK-HEP1	IC₅₀	70.4 μM Compound: Gomisin C	Antiproliferative activity against human SK-HEP1 cells after 72 hrs by SRB assay Antiproliferative activity against human SK-HEP1 cells after 72 hrs by SRB assay	[PMID: 18063366]
SNU-638	IC₅₀	44.7 μM Compound: Gomisin C	Antiproliferative activity against human SNU638 cells after 72 hrs by SRB assay Antiproliferative activity against human SNU638 cells after 72 hrs by SRB assay	[PMID: 18063366]
T47D	IC₅₀	51.2 μM Compound: Gomisin C	Antiproliferative activity against human T47D cells after 72 hrs by SRB assay Antiproliferative activity against human T47D cells after 72 hrs by SRB assay	[PMID: 18063366]

In Vitro

The concentrations of TNF-α and IL-6 in the supernatant of cells pretreated with 2.5 or 25 mg/L of Schisantherin A are significantly decreased compared to the LPS control group (p<0.05, p<0.01). The potential cytotoxicity of Schisantherin A is evaluated by the MTT assay after incubating cells for 24 h in the absence or presence of LPS, result shows cell viabilities are not affected by the cytokines at concentrations used (0.5, 2.5, 25 mg/L). RAW 264.7 murine macrophage cells are pre-incubated with Schisantherin A for 1 h and then stimulated with 1 mg/L LPS for 12 h. Both LPS and samples are untreated in control group. After the cell culture media are collected, nitrite and PGE₂ levels are determined, and Schisantherin A is found to reduce NO and PGE₂ production in a dose-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Schisantherin A, a dibenzocyclooctadiene lignan isolated from the fruit of Schisandra sphenanthera, has been reported to possess varied beneficial pharmacological effects. Schisantherin A protects lipopolysaccharide-induced acute respiratory distress syndrome in mice through inhibiting NF-κB and MAPKs signaling pathways. Pretreatment with Schisantherin A markedly ameliorates LPS-induced histopathologic changes and decreases the levels of TNF-α, IL-6 and IL-1β in the BALF. In addition, the phosphorylation of NF-κB p65, IκB-α, JNK, ERK and p38 induced by LPS are suppressed by Schisantherin A. The lung wet/dry weight ratio is evaluated at 7 h after the intranasal instillation of LPS. The results show that there are no differences between control group and Schisantherin A (40 mg/kg) group (p>0.05). LPS causes a significant increase in lung wet/dry weight ratio (p<0.01) compared with the control group. Schisantherin A dose-dependently decreases the lung wet/dry weight ratio (p<0.05) compared to those in the LPS group^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

536.57

Formula

C₃₀H₃₂O₉

CAS No.

58546-56-8

Appearance

Solid

Color

White to off-white

SMILES

O[C@]([C@@H](C)C1)(C)[C@@H](OC(C2=CC=CC=C2)=O)C3=CC(OC)=C(OC)C(OC)=C3C4=C1C=C5OCOC5=C4OC

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (186.37 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8637 mL	9.3184 mL	18.6369 mL
5 mM	0.3727 mL	1.8637 mL	3.7274 mL
10 mM	0.1864 mL	0.9318 mL	1.8637 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.66 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.66 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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mg/kg

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.87%

Select Batch:

Data Sheet (285 KB) SDS (393 KB)

COA (258 KB) HNMR (278 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Ci X, et al. Schisantherin A exhibits anti-inflammatory properties by down-regulating NF-kappaB and MAPK signaling pathways in lipopolysaccharide-treated RAW 264.7 cells. Inflammation. 2010 Apr;33(2):126-36. [Content Brief]

[2]. Zhou E, et al. Schisantherin A protects lipopolysaccharide-induced acute respiratory distress syndrome in mice through inhibiting NF-κB and MAPKs signaling pathways. Int Immunopharmacol. 2014 Sep;22(1):133-40. [Content Brief]

Cell Assay ^[1]	The MTT assay is performed to measure cell viability. RAW 264.7 cells are mechanically scraped, seeded in 96-well plates at 4×10⁵ cells/mL, and incubated in a 37°C, 5% CO₂ incubator overnight. After 24 h, cells, treated with 50 μL of different concentrations of Schisantherin A (0-25 mg/L) for 1 h are then stimulated with 50 μL LPS for 18 h. Subsequently, 20 μL of 5 mg/mL MTT in FBS-free medium is added to each well, and the cells are incubated for 4 h. MTT is removed and resolved with 150 μL/well DMSO. The optical density is measured at 570 nm using a microplate reader. Concentrations are determined for three wells of each sample, and this experiment done in triplicate^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[1]	Mice^[1] Male BALB/c mice, 6-8 weeks old, are used. All mice are randomly divided into six groups: control group, Schisantherin A (40 mg/kg) group, LPS group, Schisantherin A (10, 20 and 40 mg/kg)+LPS group, and Dexamethasone (DEX)+LPS group. DEX+LPS group is used as a positive control. Schisantherin A and DEX (5 mg/kg) are conducted intraperitoneally. Mice of control and LPS groups are given an equal volume of PBS. One hour later, after slightly anesthetized with an inhalation of diethyl ether, mice are instilled intranasally (i.n.) 10 μg LPS in 50 μL PBS to induce lung injury. Control mice are given 50 μL PBS instead of LPS. All mice are alive after 7 h of LPS treatment^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Ci X, et al. Schisantherin A exhibits anti-inflammatory properties by down-regulating NF-kappaB and MAPK signaling pathways in lipopolysaccharide-treated RAW 264.7 cells. Inflammation. 2010 Apr;33(2):126-36. [Content Brief] [2]. Zhou E, et al. Schisantherin A protects lipopolysaccharide-induced acute respiratory distress syndrome in mice through inhibiting NF-κB and MAPKs signaling pathways. Int Immunopharmacol. 2014 Sep;22(1):133-40. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.8637 mL	9.3184 mL	18.6369 mL	46.5922 mL
	5 mM	0.3727 mL	1.8637 mL	3.7274 mL	9.3184 mL
	10 mM	0.1864 mL	0.9318 mL	1.8637 mL	4.6592 mL
	15 mM	0.1242 mL	0.6212 mL	1.2425 mL	3.1061 mL
	20 mM	0.0932 mL	0.4659 mL	0.9318 mL	2.3296 mL
	25 mM	0.0745 mL	0.3727 mL	0.7455 mL	1.8637 mL
	30 mM	0.0621 mL	0.3106 mL	0.6212 mL	1.5531 mL
	40 mM	0.0466 mL	0.2330 mL	0.4659 mL	1.1648 mL
	50 mM	0.0373 mL	0.1864 mL	0.3727 mL	0.9318 mL
	60 mM	0.0311 mL	0.1553 mL	0.3106 mL	0.7765 mL
	80 mM	0.0233 mL	0.1165 mL	0.2330 mL	0.5824 mL
	100 mM	0.0186 mL	0.0932 mL	0.1864 mL	0.4659 mL

Schisantherin A Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Schisantherin A58546-56-8Gomisin-C Schizantherin-A Wuweizi ester-ANF-κBNuclear factor-κBNuclear factor-kappaBInhibitorinhibitorinhibit

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Schisantherin A (Synonyms: Gomisin-C; Schizantherin-A; Wuweizi ester-A)

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Schisantherin A Related Antibodies

1 Publications Citing Use of MCE Schisantherin A

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Complete Stock Solution Preparation Table

Schisantherin A Related Classifications

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