Solamargine (Synonyms: Solamargin; δ-Solanigrine)

Name: Solamargine
Brand: MedChemExpress
SKU: HY-N0069
Rating: 5.0 (12 reviews)

Cat. No.: HY-N0069 Purity: 99.97%: FAQs
Data Sheet SDS COA Handling Instructions

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Solamargine, a derivative from the steroidal solasodine in Solanum species, exhibits anticancer activities in numerous types of cancer. Solamargine induces non-selective cytotoxicity and P-glycoprotein inhibition. Solamargine significantly inhibits migration and invasion of HepG2 cells by down-regulating MMP-2 and MMP-9 expression and activity.

For research use only. We do not sell to patients.

Solamargine Chemical Structure

CAS No. : 20311-51-7

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 206	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 206	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 68	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 144	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 216	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 816	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Customer Review

Based on 12 publication(s) in Google Scholar

11 Publications Citing Use of MCE Solamargine

: Solamargine purchased from MedChemExpress. Usage Cited in: Int J Oncol. 2019 Mar;54(3):905-915. [Abstract]; Western blot analysis for the protein expression of XIAP, survivin, Ki‑67, PCNA and cyclin D1 in the treatment of MOCK, solamargine and Fe_{3_{O₄-solamargine.}}

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	8 μM Compound: 1, Solamargine	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	[PMID: 21482107]
A549	IC₅₀	8.18 μM Compound: SM	Cytotoxicity against human A549 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human A549 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 24534485]
H9c2	IC₅₀	> 20 μM Compound: 1, Solamargine	Cytotoxicity against rat H9c2 cells by MTT assay Cytotoxicity against rat H9c2 cells by MTT assay	[PMID: 21482107]
HCT-116	IC₅₀	3.8 μM Compound: 1, Solamargine	Cytotoxicity against human HCT116 cells by MTT assay Cytotoxicity against human HCT116 cells by MTT assay	[PMID: 21482107]
HeLa	IC₅₀	6 μM Compound: 1, Solamargine	Cytotoxicity against human HeLa cells by MTT assay Cytotoxicity against human HeLa cells by MTT assay	[PMID: 21482107]
HepG2	IC₅₀	2.5 μM Compound: 1, Solamargine	Cytotoxicity against human HepG2 cells by MTT assay Cytotoxicity against human HepG2 cells by MTT assay	[PMID: 21482107]
HepG2	IC₅₀	2.67 μM Compound: SM	Cytotoxicity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 24534485]
K562	IC₅₀	5.2 μM Compound: 1, Solamargine	Cytotoxicity against human K562 cells by MTT assay Cytotoxicity against human K562 cells by MTT assay	[PMID: 21482107]
L02	IC₅₀	13.23 μM Compound: SM	Cytotoxicity against human HL-7702 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human HL-7702 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 24534485]
L02	IC₅₀	13.5 μM Compound: 1, Solamargine	Cytotoxicity against human HL-7702 by MTT assay Cytotoxicity against human HL-7702 by MTT assay	[PMID: 21482107]
MCF7	IC₅₀	2.1 μM Compound: 1, Solamargine	Cytotoxicity against human MCF7 cells by MTT assay Cytotoxicity against human MCF7 cells by MTT assay	[PMID: 21482107]
MCF7	IC₅₀	8.12 μM Compound: SM	Cytotoxicity against human MCF7 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 24534485]
PLC-PRF-5	ED₅₀	1.53 μg/mL Compound: solamargine	Cytotoxicity against human PLC/PRF/5 cells by microassay Cytotoxicity against human PLC/PRF/5 cells by microassay	[PMID: 2380724]
U-87MG ATCC	IC₅₀	3.2 μM Compound: 1, Solamargine	Cytotoxicity against human U87 cells by MTT assay Cytotoxicity against human U87 cells by MTT assay	[PMID: 21482107]

In Vitro

Solamargine (15 μg/ml; 72 hours) predominantly stimulates the cells of the G2/M phase to apoptosis^[3].
Solamargine (72 hours) dramatically inhibits the proliferation of SMMC-7721 and HepG2 cells in a dose- and time-dependent manner. The IC₅₀s are 9.21 and 19.88 μg/ml, respectively^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:	SMMC-7721cells
Concentration:	15 μg/ml
Incubation Time:	72 hours
Result:	Displayed a significant increase of sub-G1.

In Vivo

Solamargine (10 mg/kg; intragastric administration; once daily for 8 days) exhibits an antitumor effect and promotes the apoptosis of GC in vivo^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female specific pathogen-free BALB/c nude mice weighing 18-20 g (6-8-weeks-old)^[4]
Dosage:	10 mg/kg
Administration:	Intragastric administration; once daily for 8 days
Result:	Tumor growth was significantly inhibited .

Molecular Weight

868.06

Formula

C₄₅H₇₃NO₁₅

CAS No.

20311-51-7

Appearance

Solid

Color

White to off-white

SMILES

C[C@H](CC1)CN[C@]21O[C@@]3([H])C[C@@]4([H])[C@]5([H])CC=C6C[C@@H](O[C@@]7([H])[C@H](O[C@@]8([H])[C@H](O)[C@H](O)[C@@H](O)[C@H](C)O8)[C@@H](O)[C@H](O[C@]9([H])O[C@@H](C)[C@H](O)[C@@H](O)[C@H]9O)[C@@H](CO)O7)CC[C@]6(C)[C@@]5([H])CC[C@]4(C)[C@@]3([H])[C@@H]2C

Structure Classification

Saccharides

Steroids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (115.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.1520 mL	5.7600 mL	11.5199 mL
5 mM	0.2304 mL	1.1520 mL	2.3040 mL
10 mM	0.1152 mL	0.5760 mL	1.1520 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (2.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (2.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.97%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (253 KB) HNMR (362 KB) RP-HPLC (213 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Burger T, et al. Solamargine, a bioactive steroidal alkaloid isolated from Solanum aculeastrum induces non-selective cytotoxicity and P-glycoprotein inhibition. BMC Complement Altern Med. 2018 May 2;18(1):137. [Content Brief]

[2]. Sani IK, et al. Solamargine inhibits migration and invasion of human hepatocellular carcinoma cells through down-regulation of matrix metalloproteinases 2 and 9 expression and activity. Toxicol In Vitro. 2015 Aug;29(5):893-900. [Content Brief]

[3]. Ding X, et al. Induction of apoptosis in human hepatoma SMMC-7721 cells by solamargine from Solanum nigrum L. J Ethnopharmacol. 2012 Jan 31;139(2):599-604. [Content Brief]

[4]. Fu R, et al. Solamargine inhibits gastric cancer progression by regulating the expression of lncNEAT1_2 via the MAPK signaling pathway. Int J Oncol. 2019 May;54(5):1545-1554. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.1520 mL	5.7600 mL	11.5199 mL	28.7999 mL
	5 mM	0.2304 mL	1.1520 mL	2.3040 mL	5.7600 mL
	10 mM	0.1152 mL	0.5760 mL	1.1520 mL	2.8800 mL
	15 mM	0.0768 mL	0.3840 mL	0.7680 mL	1.9200 mL
	20 mM	0.0576 mL	0.2880 mL	0.5760 mL	1.4400 mL
	25 mM	0.0461 mL	0.2304 mL	0.4608 mL	1.1520 mL
	30 mM	0.0384 mL	0.1920 mL	0.3840 mL	0.9600 mL
	40 mM	0.0288 mL	0.1440 mL	0.2880 mL	0.7200 mL
	50 mM	0.0230 mL	0.1152 mL	0.2304 mL	0.5760 mL
	60 mM	0.0192 mL	0.0960 mL	0.1920 mL	0.4800 mL
	80 mM	0.0144 mL	0.0720 mL	0.1440 mL	0.3600 mL
	100 mM	0.0115 mL	0.0576 mL	0.1152 mL	0.2880 mL

Solamargine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Solamargine (Synonyms: Solamargin; δ-Solanigrine)

Customer Review

11 Publications Citing Use of MCE Solamargine

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Solamargine (Synonyms: Solamargin; δ-Solanigrine)

Customer Review

Solamargine Related Antibodies

11 Publications Citing Use of MCE Solamargine

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Solamargine Related Antibodies

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