2. Metabolic Enzyme/Protease
  3. HIF/HIF Prolyl-Hydroxylase
  4. THS-044

THS-044 binding stabilizes the HIF2α PAS-B folded state, for regulating HIF2 activity in endogenous and clinical settings.

For research use only. We do not sell to patients.

THS-044 Chemical Structure

THS-044 Chemical Structure

CAS No. : 62054-67-5

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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10 mM * 1 mL in DMSO USD 384 In-stock
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10 mg USD 420 In-stock
50 mg USD 980 In-stock
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Based on 1 publication(s) in Google Scholar

THS-044 Related Antibodies

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HIF-1α HIF

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Description

THS-044 binding stabilizes the HIF2α PAS-B folded state, for regulating HIF2 activity in endogenous and clinical settings. Target: HIF2α Limited trypsin proteolysis reveals that both apo and THS-044-bound protein are efficiently cut at R330 in the extended HI loop. In the THS-044 bound state, there appears no additional proteolysis at the remaining candidate trypsin sites. In contrast, these THS-044-protected sites are protease accessible in the unliganded protein, leading its complete degradation. In parallel, NMR-based deuterium exchange measurements revealed a dramatic stabilization of the THS-044-bound protein β-sheet, with some sites experiencing 100-fold enhanced protection factors relative to the ligand-free protein.

Molecular Weight

291.23

Formula

C11H12F3N3O3
CAS No.
Appearance

Solid

Color

Yellow to orange

SMILES

FC(C1=CC=C(NN2CCOCC2)C([N+]([O-])=O)=C1)(F)F
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (429.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4337 mL 17.1686 mL 34.3371 mL
5 mM 0.6867 mL 3.4337 mL 6.8674 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.67%

SDS (252 KB)

COA (251 KB) LCMS (92 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.4337 mL 17.1686 mL 34.3371 mL 85.8428 mL
5 mM 0.6867 mL 3.4337 mL 6.8674 mL 17.1686 mL
10 mM 0.3434 mL 1.7169 mL 3.4337 mL 8.5843 mL
15 mM 0.2289 mL 1.1446 mL 2.2891 mL 5.7229 mL
20 mM 0.1717 mL 0.8584 mL 1.7169 mL 4.2921 mL
25 mM 0.1373 mL 0.6867 mL 1.3735 mL 3.4337 mL
30 mM 0.1145 mL 0.5723 mL 1.1446 mL 2.8614 mL
40 mM 0.0858 mL 0.4292 mL 0.8584 mL 2.1461 mL
50 mM 0.0687 mL 0.3434 mL 0.6867 mL 1.7169 mL
60 mM 0.0572 mL 0.2861 mL 0.5723 mL 1.4307 mL
80 mM 0.0429 mL 0.2146 mL 0.4292 mL 1.0730 mL
100 mM 0.0343 mL 0.1717 mL 0.3434 mL 0.8584 mL
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THS-044 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

THS-04462054-67-5THS044THS 044HIF/HIF Prolyl-HydroxylaseHypoxia-inducible factorsHIFsHIF-PHInhibitorinhibitorinhibit

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