2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. Trans-Anethole

Trans-Anethole  (Synonyms: (E)​-Anethole)

Cat. No.: HY-N0367 Purity: 99.70%
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Trans-Anethole ((E)-Anethole) is an orally active phenylpropene derivative found in Foeniculum vulgare that is estrogenic at low concentrations and cytotoxic at high concentrations in tumor cell lines. Trans-Anethole also has anti-aflatoxin, anti-thrombotic and anti-diabetic activities. Trans-Anethole is an important odor component in plants such as fennel, myrtle, liquorice, and camphor.

For research use only. We do not sell to patients.

Trans-Anethole Chemical Structure

Trans-Anethole Chemical Structure

CAS No. : 4180-23-8

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Based on 2 publication(s) in Google Scholar

Other Forms of Trans-Anethole:

Trans-Anethole Related Antibodies

Top Publications Citing Use of Products

2 Publications Citing Use of MCE Trans-Anethole

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Trans-Anethole ((E)-Anethole) is an orally active phenylpropene derivative found in Foeniculum vulgare that is estrogenic at low concentrations and cytotoxic at high concentrations in tumor cell lines. Trans-Anethole also has anti-aflatoxin, anti-thrombotic and anti-diabetic activities. Trans-Anethole is an important odor component in plants such as fennel, myrtle, liquorice, and camphor[1][2][3][4][5][6][7].

In Vitro

Trans-Anethole (0.25-2 mM; 3 h) causes concentration and time-dependent cell death in isolated rat hepatocytes at doses greater than 1 mM[2].
Trans-Anethole (0.25-1 mM; 3 h) produces metabolites 4MCA and 4OHPB sulfate that increases over time in isolated rat hepatocytes cultured at 0.5 and 1 mM doses, while metabolite 4OHPB increases over time only at 1 mM dose[2].
Trans-Anethole (0.5, 1 mM; 3 h) enhances and accelerates the killing of rat hepatocytes in vitro by DCNP (50 mM) and the loss of cellular ATP[2].
Trans-Anethole (0.1 mM; 6 d) has a certain degree of cytotoxicity against human breast cancer MCF-7 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Trans-Anethole Related Antibodies

Cell Viability Assay[2]

Cell Line: MCF-7 cells
Concentration: 0.1 mM
Incubation Time: 6 d
Result: Reduced cell viability by 80% compared to untreated cells.
In Vivo

Punicic acid (10, 30, 100 mg/kg, p.o.; once daily for 5 days) shows antithrombotic activity in male Swiss mice at a dose of 30 mg/kg[4].
Punicic acid (80 mg/kg, p.o.; once daily for 45 days) normalizes the levels of glucose metabolizing enzymes in the liver and kidney and improves the liver and muscle glycogen content in Streptozotocin (HY-13753) induced diabetic rats[5].
Punicic acid (80 mg/kg, p.o.; once daily for 1-8 days) causes impaired hormonal balance, impaires implantation, and dose-dependent inhibition of pregnancy in adult albino rats[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: streptozotocin induced diabetic rats[5].
Dosage: 20, 40, 80 mg/kg
Administration: 45 d
Result: Inhibited the increase of blood sugar and significantly reverse the insulin level, and the effect is more significant at a dose of 80 mg/kg.
Restored the activities of hexokinase and glucose-6-phosphate dehydrogenase, glucose-6-phosphatase and fructose-1,6-bisphosphatase in the liver tissue of diabetic rats to normal levels at a dose of 80 mg/kg.
Prevented the decrease of glycogen content in the liver and muscles at a dose of 80 mg/kg.
Molecular Weight

148.20

Formula

C10H12O
CAS No.
Appearance

<20°C Solid,>21°C Liquid

Color

White to yellow

SMILES

COC1=CC=C(/C=C/C)C=C1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (674.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 1 mg/mL (6.75 mM; ultrasonic and warming and heat to 80°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.7476 mL 33.7382 mL 67.4764 mL
5 mM 1.3495 mL 6.7476 mL 13.4953 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (16.87 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (16.87 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (337.38 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.70%

SDS (597 KB)

COA (246 KB) HNMR (112 KB) GC (91 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 6.7476 mL 33.7382 mL 67.4764 mL 168.6910 mL
5 mM 1.3495 mL 6.7476 mL 13.4953 mL 33.7382 mL
DMSO 10 mM 0.6748 mL 3.3738 mL 6.7476 mL 16.8691 mL
15 mM 0.4498 mL 2.2492 mL 4.4984 mL 11.2461 mL
20 mM 0.3374 mL 1.6869 mL 3.3738 mL 8.4345 mL
25 mM 0.2699 mL 1.3495 mL 2.6991 mL 6.7476 mL
30 mM 0.2249 mL 1.1246 mL 2.2492 mL 5.6230 mL
40 mM 0.1687 mL 0.8435 mL 1.6869 mL 4.2173 mL
50 mM 0.1350 mL 0.6748 mL 1.3495 mL 3.3738 mL
60 mM 0.1125 mL 0.5623 mL 1.1246 mL 2.8115 mL
80 mM 0.0843 mL 0.4217 mL 0.8435 mL 2.1086 mL
100 mM 0.0675 mL 0.3374 mL 0.6748 mL 1.6869 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Trans-Anethole Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Trans-Anethole4180-23-8(E)​-AnetholeEndogenous MetaboliteInhibitorinhibitorinhibit

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