Tylosin tartrate

Name: Tylosin tartrate
Brand: MedChemExpress
SKU: HY-B0519
Rating: 4.5 (1 reviews)

Cat. No.: HY-B0519 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Tylosin tartrate is a macrolide antibiotic found naturally as a fermentation product of Streptomyces fradiae. Tylosin tartrate exerts potent antimicrobial activity against Gram-positive bacteria. Tylosin tartrate is widely used as a feed additive for promoting animal growth. Tylosin tartrate is used for veterinary purposes against bacterial dysentery and respiratory diseases in poultry, pigs and cattle.

For research use only. We do not sell to patients.

Tylosin tartrate Chemical Structure

CAS No. : 74610-55-2

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 79	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 79	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	USD 72	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 90	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
1 g	USD 300	In-stock	Estimated Time of Arrival: December 31
5 g	USD 600	In-stock	Estimated Time of Arrival: December 31
10 g	USD 900	In-stock	Estimated Time of Arrival: December 31
50 g		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Tylosin tartrate:

Tylosin In-stock
Tylosin phosphate In-stock
Tylosin lactate Get quote

1 Publications Citing Use of MCE Tylosin tartrate

•Chemosphere. 2019 Jun;225:378-387. [Abstract]

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Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Macrolide

In Vitro

Tylosin tartrate exerts antibacterial effects by binding to 23S rRNA of the bacterial ribosomal 50S subunit ^[1].
Tylosin tartrate also prevents growth of Gram-negative strains, with MICs of 64 μg/mL, 32 μg/mL, 512 μg/mL and 1 μg/mL for M. haemolytica 11935, P. multocida 4407, E. coli ATCC 25922 and E. coli AS19rlmA^I, respectively^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Tylosin tartrate (10-500 mg/kg; s.c.) generally suppresses the elevated TNF-α and IL-1β levels and increases the IL-10 levels in the Lipopolysaccharide (LPS) -treated animals^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Balb/C mice (2-3 months old, 20-25 g)^[4]
Dosage:	10 mg/kg, 100 mg/kg, 500 mg/kg
Administration:	Subcutaneous injection
Result:	Reduced the elevated TNF-α and IL-1β in LPS (250 µg)-treated mice but increased their IL-10 levels.

Molecular Weight

1066.19

Formula

C₅₀H₈₃NO₂₃

CAS No.

74610-55-2

Appearance

Solid

Color

White to off-white

SMILES

C[C@@H](O[C@@]1([H])O[C@H]([C@H]([C@@H](CC2=O)O)C)[C@H](C[C@H](C(/C=C/C(C)=C/[C@H](CO[C@H](O[C@H](C)[C@@H](O)[C@H]3OC)[C@@H]3OC)[C@@H](CC)O2)=O)C)CC=O)[C@H]([C@@H]([C@H]1O)N(C)C)O[C@@](O[C@@H](C)[C@@H]4O)([H])C[C@]4(O)C.O[C@@H](C(O)=O)[C@@H](O)C(O)=O

Structure Classification

Initial Source

Microorganisms

Streptomyces fradiae

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : ≥ 100 mg/mL (93.79 mM)

DMSO : ≥ 100 mg/mL (93.79 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.9379 mL	4.6896 mL	9.3792 mL
5 mM	0.1876 mL	0.9379 mL	1.8758 mL
10 mM	0.0938 mL	0.4690 mL	0.9379 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.34 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (2.34 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (2.34 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (93.79 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (281 KB) SDS (252 KB)

COA (257 KB) HNMR (379 KB)

Handling Instructions (2659 KB)

References

[1]. Mingfu Liu, et al. Resistance to the macrolide antibiotic tylosin is conferred by single methylations at 23S rRNA nucleotides G748 and A2058 acting in synergy. Proc Natl Acad Sci U S A. 2002 Nov 12; 99(23): 14658–14663. [Content Brief]

[2]. Carlo Pinna, et al. In Vitro Evaluation of the Effects of Tylosin on the Composition and Metabolism of Canine Fecal Microbiota. Animals (Basel). 2020 Jan; 10(1): 98. [Content Brief]

[3]. Niels Møller Andersen, et al. Inhibition of Protein Synthesis on the Ribosome by Tildipirosin Compared with Other Veterinary Macrolides. Antimicrob Agents Chemother. 2012 Nov; 56(11): 6033–6036. [Content Brief]

[4]. Ayse Er, et al. Effects of tylosin on serum cytokine levels in healthy and lipopolysaccharide-treated mice. Acta Vet Hung. 2010 Mar;58(1):75-81. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	0.9379 mL	4.6896 mL	9.3792 mL	23.4480 mL
	5 mM	0.1876 mL	0.9379 mL	1.8758 mL	4.6896 mL
	10 mM	0.0938 mL	0.4690 mL	0.9379 mL	2.3448 mL
	15 mM	0.0625 mL	0.3126 mL	0.6253 mL	1.5632 mL
	20 mM	0.0469 mL	0.2345 mL	0.4690 mL	1.1724 mL
	25 mM	0.0375 mL	0.1876 mL	0.3752 mL	0.9379 mL
	30 mM	0.0313 mL	0.1563 mL	0.3126 mL	0.7816 mL
	40 mM	0.0234 mL	0.1172 mL	0.2345 mL	0.5862 mL
	50 mM	0.0188 mL	0.0938 mL	0.1876 mL	0.4690 mL
	60 mM	0.0156 mL	0.0782 mL	0.1563 mL	0.3908 mL
	80 mM	0.0117 mL	0.0586 mL	0.1172 mL	0.2931 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.