2. Epigenetics Stem Cell/Wnt Protein Tyrosine Kinase/RTK JAK/STAT Signaling Cell Cycle/DNA Damage NF-κB Immunology/Inflammation
  3. JAK HDAC NF-κB Toll-like Receptor (TLR) MyD88 Interleukin Related
  4. UF010

UF010 is a selective inhibitor of class I HDAC. UF010 has cytotoxicity to cancer cells and reduces neuroinflammation in the hippocampus. UF010 can be used for the research of neurological diseases.

For research use only. We do not sell to patients.

UF010 Chemical Structure

UF010 Chemical Structure

CAS No. : 537672-41-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 39 In-stock
Solution
10 mM * 1 mL in DMSO USD 39 In-stock
Solid
5 mg USD 35 In-stock
10 mg USD 50 In-stock
25 mg USD 90 In-stock
50 mg USD 140 In-stock
100 mg USD 220 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

UF010 Related Antibodies

Top Publications Citing Use of Products

View All JAK Isoform Specific Products:

View All Isoforms
JAK1 JAK2 JAK3 Tyk2 JAK

View All HDAC Isoform Specific Products:

View All Isoforms
HDAC HDAC1 HDAC2 HDAC3 HDAC4 HDAC5 HDAC6 HDAC7 HDAC8 HDAC9 HDAC10 HDAC11 HD1 HD2

View All NF-κB Isoform Specific Products:

View All Isoforms
NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

View All Toll-like Receptor (TLR) Isoform Specific Products:

View All Isoforms
TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

View All Interleukin Related Isoform Specific Products:

View All Isoforms
IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

UF010 is a selective inhibitor of class I HDAC. UF010 has cytotoxicity to cancer cells and reduces neuroinflammation in the hippocampus. UF010 can be used for the research of neurological diseases[1][2][3].

IC50 & Target[1]

HDAC1

1.42 μM ()

HDAC2

0.32 μM (IC50)

HDAC3

256.7 nM (IC50)

HDAC6

18.93 μM (IC50)

HDAC8

3.97 μM (IC50)

IL-6

 

Cellular Effect
Cell Line Type Value Description References
4T1 IC50
8.4 μM
Compound: UF010
Cytotoxicity against mouse 4T1 cells assessed as inhibition of cell viability for 72 hrs by MTT assay
Cytotoxicity against mouse 4T1 cells assessed as inhibition of cell viability for 72 hrs by MTT assay
[PMID: 35635949]
A549 IC50
20.81 μM
Compound: UF010
Cytotoxicity against human A549 cells assessed as inhibition of cell viability for 72 hrs by MTT assay
Cytotoxicity against human A549 cells assessed as inhibition of cell viability for 72 hrs by MTT assay
[PMID: 35635949]
B16-F10 IC50
2.41 μM
Compound: UF010
Cytotoxicity against mouse B16-F10 cells assessed as inhibition of cell viability for 72 hrs by MTT assay
Cytotoxicity against mouse B16-F10 cells assessed as inhibition of cell viability for 72 hrs by MTT assay
[PMID: 35635949]
HEK293 IC50
98.52 μM
Compound: UF010
Cytotoxicity against human HEK293 cells assessed as inhibition of cell viability for 72 hrs by MTT assay
Cytotoxicity against human HEK293 cells assessed as inhibition of cell viability for 72 hrs by MTT assay
[PMID: 35635949]
MCF7 IC50
17.93 μM
Compound: UF010
Cytotoxicity against human MCF7 cells assessed as inhibition of cell viability for 72 hrs by MTT assay
Cytotoxicity against human MCF7 cells assessed as inhibition of cell viability for 72 hrs by MTT assay
[PMID: 35635949]
RAW264.7 IC50
121.1 μM
Compound: UF010
Cytotoxicity against mouse RAW264.7 cells assessed as inhibition of cell viability for 72 hrs by MTT assay
Cytotoxicity against mouse RAW264.7 cells assessed as inhibition of cell viability for 72 hrs by MTT assay
[PMID: 35635949]
In Vitro

UF010 (500 nM, 4 days) leads to a signifcant reduction in rod production with a concomitant increase in Müller cells[1].
UF010 (10-100 μM, 72 h) has cytotoxicity to B16F10 cells, MCF-7 cells, A549 cells, 4T1 cells, HEK-293 cells and HCEC cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

UF010 Related Antibodies

Cell Cytotoxicity Assay [2]

Cell Line: B16F10 cells, MCF-7 cells, A549 cells, 4T1 cells, HEK-293 cells and HCEC cells
Concentration: 10 μM, 100 μM
Incubation Time: 72 h
Result: Showed IC50 values of 2.41 μM for B16F10 cells, 20.81 μM for A549 cells, 17.93 μM for MCF-7 cells, 8.40 μM for 4T1 cells, 98.52 μM for HEK-293 cells, 95.4 μM for HCEC cells.
In Vivo

UF010 (15 mg/kg, Intraperitoneal injection, single dose) contributes considerably to the inflammatory regulation of hippocampal neurons in postoperative cognitive dysfunction (POCD) mice[4].
UF010 (15 mg/kg, Intraperitoneal injection, single dose) has antitumor therapeutic efficacy in the 4T1-Luc tumor-bearing mouse model[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: postoperative cognitive dysfunction (POCD) mice[4]
Dosage: 15 mg/kg
Administration: Intraperitoneal injection (i.p.)
Result: Weakened the infiltration of CD4+ T cells and NK cells in hippocampal tissues.
Reduced inflammatory parameters in serum and hippocampal tissues, such as interleukin 6 (IL-6), C-reactive protein (CRP), and tumor necrosis factor alpha (TNF-α) levels.
Activated the NF-κB/p65, JAK/STAT and TLR/MyD88 pathways.
Animal Model: 4T1-Luc tumor-bearing mouse model[5]
Dosage: 15 mg/kg
Administration: Intraperitoneal injection (i.p.)
Result: Inhibited the tumor growth rate percentage to 55.56, 38.36, 39.52% at days 7, 14, and 21.
Induced high levels of ROS generation, causing apoptosis-mediated tumor cell death.
Molecular Weight

271.15

Formula

C11H15BrN2O
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NNCCCC)C1=CC=C(Br)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (368.80 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.6880 mL 18.4400 mL 36.8800 mL
5 mM 0.7376 mL 3.6880 mL 7.3760 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (9.22 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (9.22 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.6880 mL 18.4400 mL 36.8800 mL 92.1999 mL
5 mM 0.7376 mL 3.6880 mL 7.3760 mL 18.4400 mL
10 mM 0.3688 mL 1.8440 mL 3.6880 mL 9.2200 mL
15 mM 0.2459 mL 1.2293 mL 2.4587 mL 6.1467 mL
20 mM 0.1844 mL 0.9220 mL 1.8440 mL 4.6100 mL
25 mM 0.1475 mL 0.7376 mL 1.4752 mL 3.6880 mL
30 mM 0.1229 mL 0.6147 mL 1.2293 mL 3.0733 mL
40 mM 0.0922 mL 0.4610 mL 0.9220 mL 2.3050 mL
50 mM 0.0738 mL 0.3688 mL 0.7376 mL 1.8440 mL
60 mM 0.0615 mL 0.3073 mL 0.6147 mL 1.5367 mL
80 mM 0.0461 mL 0.2305 mL 0.4610 mL 1.1525 mL
100 mM 0.0369 mL 0.1844 mL 0.3688 mL 0.9220 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

UF010 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

UF010537672-41-6UF 010UF-010JAKHDACNF-κBToll-like Receptor (TLR)MyD88Interleukin RelatedJanus kinaseHistone deacetylasesNuclear factor-κBNuclear factor-kappaBILB16F10 cellsMCF-7 cellsA549 cells4T1 cellsHEK-293 cellsHCEC cellsPOCDInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
UF010
Cat. No.:
HY-18976
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (392 KB) Français - FR (392 KB) Deutsch - DE (392 KB) Norwegian - NO (392 KB) Español - ES (392 KB) Swedish - SV (392 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.