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  5. Urethane

Urethane  (Synonyms: Ethyl carbamate; Carbamic acid ethyl ester; Ethylurethane)

Cat. No.: HY-B1207 Purity: 99.08%
SDS COA Handling Instructions

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Urethane (Ethyl carbamate), the ethyl ester of carbamic acid, is a byproduct of fermentation found in various food products. Urethane has the ability to suppress bacterial, protozoal, sea urchin egg, and plant tissue growth in vitro.

For research use only. We do not sell to patients.

Urethane Chemical Structure

Urethane Chemical Structure

CAS No. : 51-79-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 28 In-stock
Solution
10 mM * 1 mL in DMSO USD 28 In-stock
Solid
5 g USD 25 In-stock
10 g USD 40 In-stock
50 g   Get quote  

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Based on 2 publication(s) in Google Scholar

Other Forms of Urethane:

Urethane Related Antibodies

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Description

Urethane (Ethyl carbamate), the ethyl ester of carbamic acid, is a byproduct of fermentation found in various food products. Urethane has the ability to suppress bacterial, protozoal, sea urchin egg, and plant tissue growth in vitro[1].

In Vitro

Urethane is a good clastogen in mammalian somatic cells in vivo, but it shows variable results with cells in vitro. Urethane efficiently induces sister chromatid exchanges in a variety of cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Urethane Related Antibodies
In Vivo

PLP (139-151) can be used in animal modeling to construct autoimmune encephalomyelitis model.
PLP (139-151) induces acute experimental allergic encephalomyelitis (EAE) in SJL/J mice. Beginning on Day 9, the mice treated with PLP (139-151) show signs of EAE and the disease progressed rapidly to paralysis. Central nervous system inflammation, edema, gliosis, and demyelination are found in all mice killed between Days 10 and 28[2]. Young male SJL mice immunized with a major encephalitogenic peptide of myelin, PLP 139-151, develop initial clinical and histological symptoms of EAE with a severity similar to age-matched females; however, unlike females, male mice does not relapse. Significant T cell proliferation to PLP 139-151, but not to other PLP and myelin basic protein (MBP) epitopes, is observed in both males and females during the initial episode, recovery, and first relapse of clinical disease[3].
IL-17, a pro-inflammatory cytokine, plays a crucial role in the development of experimental autoimmune encephalomyelitis (EAE) induced by PLP (139-151). After subcutaneous injection of PLP (139-151) into mice, CD4 cells that specifically respond to PLP (139-151) and release pro-inflammatory cytokines migrate to and accumulate in the damaged central nervous system (CNS) regions. PLP (139-151) is used to model and establish the relapsing-remitting autoimmune encephalomyelitis (RR-EAE) model in animals[4][5].

Induction of lung tumor[1][2][3]

Background
Exposure of mouse to Urethane primarily induces tumors in lung with a single driver mutation in Kras gene at position Q61 with one substitution L or R according to mouse strain. The activation of an oncogenic Kras allele in mice was shown to be tumorigenic in multiple organs, such as liver and pancreas[2].
Specific Mmodeling Methods
Mice: hPRDX4 transgenic (Tg) and non-Tg C57BL/6 • male • 8 weeks old[1]
Administration: 1 g/kg (in 100 μL saline) • i.p. • once per week for 16 weeks or every other day for a week[1]
Note
(1) Before administration, all mice were kept in a room with relatively constant temperature (21-23℃) humidity (50-60%) , and 12 h light/dark circle. They received a standard chow diet and purified tap water ad libitum[1].
(2) After mice were sacrificed, lungs were harvested, and the number of tumors and their diameter on the lung surface were measured. Serum was also collected after centrifugation[1].
Modeling Indicators
Molecular changes: Urethane administration decreased the lavels of proapoptotic genes (BAX, BAD) no significant difference was found in antiapoptotic genes (BCL2, BCL2L1) in Tg tumor. Urethane administration elevated MMP9 and IL-1β expressions in Tg tumor[1].
Tumor changes: Urethane administration significantly increased the average number, size and diameter of tumors in PRDX4 Tg mice. Tumor apoptosis was suppressed and tumor proliferation was enhanced in Tg mice[1].
Histology analysis: Urethane administration increased microvascular permeability and macrophage infiltration in lung tumor of PRDX4 Tg mice[1].
Metabolic change: Urethane administration inhibited oxidative stress and decreased gene expressions of antioxidant enzymes (SOD1 and SOD2) in Tg mice[1].
Correlated Product(s): /
Opposite Product(s): /

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
Molecular Weight

89.09

Formula

C3H7NO2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

NC(OCC)=O
Structure Classification
Initial Source

a byproduct of fermentation
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (1122.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 11.2246 mL 56.1230 mL 112.2460 mL
5 mM 2.2449 mL 11.2246 mL 22.4492 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (23.35 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (23.35 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (561.23 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.08%

SDS (617 KB)

COA (244 KB) HNMR (110 KB) GC (193 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 11.2246 mL 56.1230 mL 112.2460 mL 280.6151 mL
5 mM 2.2449 mL 11.2246 mL 22.4492 mL 56.1230 mL
10 mM 1.1225 mL 5.6123 mL 11.2246 mL 28.0615 mL
15 mM 0.7483 mL 3.7415 mL 7.4831 mL 18.7077 mL
20 mM 0.5612 mL 2.8062 mL 5.6123 mL 14.0308 mL
25 mM 0.4490 mL 2.2449 mL 4.4898 mL 11.2246 mL
30 mM 0.3742 mL 1.8708 mL 3.7415 mL 9.3538 mL
40 mM 0.2806 mL 1.4031 mL 2.8062 mL 7.0154 mL
50 mM 0.2245 mL 1.1225 mL 2.2449 mL 5.6123 mL
60 mM 0.1871 mL 0.9354 mL 1.8708 mL 4.6769 mL
80 mM 0.1403 mL 0.7015 mL 1.4031 mL 3.5077 mL
100 mM 0.1122 mL 0.5612 mL 1.1225 mL 2.8062 mL
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Urethane Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Urethane51-79-6Ethyl carbamate Carbamic acid ethyl ester EthylurethaneBacterialParasitePesticidemutagencarbamicacidclastogennarcosisInhibitorinhibitorinhibit

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