2. Metabolic Enzyme/Protease Apoptosis
  3. Endogenous Metabolite Apoptosis
  4. Vitamin K4

Vitamin K4  (Synonyms: Acetomenaphthone)

Cat. No.: HY-B1508 Purity: 99.92%
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Vitamin K4 is a chemically synthesized Vitamin K which plays an important role in the normal blood coagulation system. Vitamin K4 arrests the cells in S phase and induces apoptosis. Vitamin K4 can be used for the research of cancer, such as prostate cancer and osteosarcoma.

For research use only. We do not sell to patients.

Vitamin K4 Chemical Structure

Vitamin K4 Chemical Structure

CAS No. : 573-20-6

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10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

Other Forms of Vitamin K4:

Vitamin K4 Related Antibodies

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  • Purity & Documentation

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Description

Vitamin K4 is a chemically synthesized Vitamin K which plays an important role in the normal blood coagulation system. Vitamin K4 arrests the cells in S phase and induces apoptosis. Vitamin K4 can be used for the research of cancer, such as prostate cancer and osteosarcoma[1][2].

In Vitro

Vitamin K4 (0-100 μM, 24 h) inhibits cell proliferation in PC-3 cells (IC50= 20.94 μM)[1].
Vitamin K4 (0-40 μM, 24 h) induces caspase-dependent apoptosis and S phase cell cycle arrest in PC-3 cells[1].
Vitamin K4 (0-100 μM, 24 h) inhibits the growth of U2OS cells in a dose-dependent manner (IC50= 25 μM)[2].
Vitamin K4 (0-35 μM, 24 h) induces apoptosis and S phase cell cycle arrest in U2OS cells[2].
Vitamin K4 (0-35 μM, 24 h) increases ROS generation and disrupts the MMP in U2OS cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Vitamin K4 Related Antibodies

Cell Migration Assay [2]

Cell Line: U2OS cells
Concentration: 0-25 μM
Incubation Time: 24 h
Result: Inhibited the migration of U2OS cells in vitro.

Western Blot Analysis[1]

Cell Line: PC-3 cells
Concentration: 0-40 μM
Incubation Time: 24 h
Result: Decreased the expression of Bcl-2.
Increased the expression of Bax, PARP, and cleaved-caspase-3.
Released cytochrome c.

Western Blot Analysis[1]

Cell Line: U2OS cells
Concentration: 0-35 μM
Incubation Time: 24 h
Result: Increased the protein expression of pro-apoptotic protein Bax, and decreased the expression of anti-apoptotic protein Bcl-2.
Molecular Weight

258.27

Formula

C15H14O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC1=CC(OC(C)=O)=C2C=CC=CC2=C1OC(C)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (387.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8719 mL 19.3596 mL 38.7192 mL
5 mM 0.7744 mL 3.8719 mL 7.7438 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (9.68 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (9.68 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.8719 mL 19.3596 mL 38.7192 mL 96.7979 mL
5 mM 0.7744 mL 3.8719 mL 7.7438 mL 19.3596 mL
10 mM 0.3872 mL 1.9360 mL 3.8719 mL 9.6798 mL
15 mM 0.2581 mL 1.2906 mL 2.5813 mL 6.4532 mL
20 mM 0.1936 mL 0.9680 mL 1.9360 mL 4.8399 mL
25 mM 0.1549 mL 0.7744 mL 1.5488 mL 3.8719 mL
30 mM 0.1291 mL 0.6453 mL 1.2906 mL 3.2266 mL
40 mM 0.0968 mL 0.4840 mL 0.9680 mL 2.4199 mL
50 mM 0.0774 mL 0.3872 mL 0.7744 mL 1.9360 mL
60 mM 0.0645 mL 0.3227 mL 0.6453 mL 1.6133 mL
80 mM 0.0484 mL 0.2420 mL 0.4840 mL 1.2100 mL
100 mM 0.0387 mL 0.1936 mL 0.3872 mL 0.9680 mL
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Vitamin K4 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Vitamin K4573-20-6AcetomenaphthoneVitamin K 4Vitamin K-4Endogenous MetaboliteApoptosiscancerblood coagulationapoptosisS phaseVitamin KInhibitorinhibitorinhibit

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