2. JAK/STAT Signaling Protein Tyrosine Kinase/RTK
  3. EGFR
  4. (E)-AG 99

(E)-AG 99  (Synonyms: (E)-Tyrphostin 46; (E)-Tyrphostin AG 99)

Cat. No.: HY-100962 Purity: 98.36%
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(E)-AG99 ((E)-Tyrphostin 46) est un inhibiteur puissant de EGFR.

(E)-AG 99 ((E)-Tyrphostin 46) is a potent EGFR inhibitor.

For research use only. We do not sell to patients.

(E)-AG 99 Chemical Structure

(E)-AG 99 Chemical Structure

CAS No. : 122520-85-8

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 In-stock
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Solid
5 mg USD 30 In-stock
10 mg USD 50 In-stock
50 mg USD 95 In-stock
100 mg USD 150 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of (E)-AG 99:

(E)-AG 99 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE (E)-AG 99

View All EGFR Isoform Specific Products:

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EGFR/ErbB1/HER1 ErbB2/HER2 ErbB3/HER3 ErbB4/HER4

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

(E)-AG 99 ((E)-Tyrphostin 46) is a potent EGFR inhibitor[1].

IC50 & Target[1]

EGFR

 

In Vitro

(E)-AG 99 effectively blocks tyrosine phosphorylation of p145met and promotes cell death accompanied by activation of caspase-like proteases[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

(E)-AG 99 Related Antibodies
Molecular Weight

204.19

Formula

C10H8N2O3
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(N)/C(C#N)=C/C1=CC=C(O)C(O)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (489.74 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.8974 mL 24.4870 mL 48.9740 mL
5 mM 0.9795 mL 4.8974 mL 9.7948 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.75 mg/mL (13.47 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.75 mg/mL (13.47 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.8974 mL 24.4870 mL 48.9740 mL 122.4350 mL
5 mM 0.9795 mL 4.8974 mL 9.7948 mL 24.4870 mL
10 mM 0.4897 mL 2.4487 mL 4.8974 mL 12.2435 mL
15 mM 0.3265 mL 1.6325 mL 3.2649 mL 8.1623 mL
20 mM 0.2449 mL 1.2243 mL 2.4487 mL 6.1217 mL
25 mM 0.1959 mL 0.9795 mL 1.9590 mL 4.8974 mL
30 mM 0.1632 mL 0.8162 mL 1.6325 mL 4.0812 mL
40 mM 0.1224 mL 0.6122 mL 1.2243 mL 3.0609 mL
50 mM 0.0979 mL 0.4897 mL 0.9795 mL 2.4487 mL
60 mM 0.0816 mL 0.4081 mL 0.8162 mL 2.0406 mL
80 mM 0.0612 mL 0.3061 mL 0.6122 mL 1.5304 mL
100 mM 0.0490 mL 0.2449 mL 0.4897 mL 1.2243 mL
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(E)-AG 99 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(E)-AG 99122520-85-8(E)-Tyrphostin 46 (E)-Tyrphostin AG 99EGFREpidermal growth factor receptorErbB-1HER1Inhibitorinhibitorinhibit

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