AEP-IN-2

Name: AEP-IN-2
Brand: MedChemExpress
SKU: HY-153986
Rating: 4.5 (1 reviews)

Cat. No.: HY-153986 Purity: 99.13%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

AEP-IN-2 is an asparagine endopeptidase (AEP) inhibitor via block AEP cleavage of APP and Tau. AEP-IN-2 has oral activity and decreases Aβ40 and Aβ42 and p-Tau levels .

For research use only. We do not sell to patients.

AEP-IN-2 Chemical Structure

CAS No. : 2565572-83-8

Size	Price	Stock	Quantity
5 mg	USD 650	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 1050	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 2100	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 3360	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

AEP-IN-2 is an asparagine endopeptidase (AEP) inhibitor via block AEP cleavage of APP and Tau. AEP-IN-2 has oral activity and decreases Aβ40 and Aβ42 and p-Tau levels ^[1].

Molecular Weight

363.39

Formula

C₁₄H₁₇N₇O₃S

CAS No.

2565572-83-8

Appearance

Solid

Color

Yellow to orange

SMILES

OCC1=C(N2CCOCC2)C3=NON=C3C(NC4=NN=C(NC)S4)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (275.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7519 mL	13.7593 mL	27.5186 mL
5 mM	0.5504 mL	2.7519 mL	5.5037 mL
10 mM	0.2752 mL	1.3759 mL	2.7519 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.13%

Select Batch:

Data Sheet (274 KB) SDS (251 KB)

COA (252 KB) HNMR (144 KB) RP-HPLC (165 KB) LCMS (104 KB)

Handling Instructions (2659 KB)

References

[1]. Zhengjiang Qian, et al. Inhibition of asparagine endopeptidase (AEP) effectively treats sporadic Alzheimer's disease in mice. Neuropsychopharmacology. 2023. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7519 mL	13.7593 mL	27.5186 mL	68.7966 mL
	5 mM	0.5504 mL	2.7519 mL	5.5037 mL	13.7593 mL
	10 mM	0.2752 mL	1.3759 mL	2.7519 mL	6.8797 mL
	15 mM	0.1835 mL	0.9173 mL	1.8346 mL	4.5864 mL
	20 mM	0.1376 mL	0.6880 mL	1.3759 mL	3.4398 mL
	25 mM	0.1101 mL	0.5504 mL	1.1007 mL	2.7519 mL
	30 mM	0.0917 mL	0.4586 mL	0.9173 mL	2.2932 mL
	40 mM	0.0688 mL	0.3440 mL	0.6880 mL	1.7199 mL
	50 mM	0.0550 mL	0.2752 mL	0.5504 mL	1.3759 mL
	60 mM	0.0459 mL	0.2293 mL	0.4586 mL	1.1466 mL
	80 mM	0.0344 mL	0.1720 mL	0.3440 mL	0.8600 mL
	100 mM	0.0275 mL	0.1376 mL	0.2752 mL	0.6880 mL

AEP-IN-2 Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AEP-IN-22565572-83-8OthersAEP InhibitorInhibitorinhibitorinhibit

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AEP-IN-2

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Complete Stock Solution Preparation Table

AEP-IN-2 Related Classifications

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