2. Cell Cycle/DNA Damage Stem Cell/Wnt PI3K/Akt/mTOR Apoptosis
  3. CDK GSK-3 Apoptosis
  4. Alsterpaullone

Alsterpaullone  (Synonyms: 9-Nitropaullone; NSC 705701)

Cat. No.: HY-108359 Purity: 99.85%
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Alsterpaullone (9-Nitropaullone) is a potent CDK inhibitor, with IC50s of 35 nM, 15 nM, 200 nM and 40 nM for CDK1/cyclin B, CDK2/cyclin A, CDK2/cyclin E and CDK5/p35, respectively. Alsterpaullone also competes with ATP for binding to GSK-3alpha/GSK-3beta with IC50s of both 4 nM. Alsterpaullone has antitumor activity, and possesses potential for the study in neurodegenerative and proliferative disorders. Alsterpaullone induces apoptosis in leukemia cell line.

For research use only. We do not sell to patients.

Alsterpaullone Chemical Structure

Alsterpaullone Chemical Structure

CAS No. : 237430-03-4

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Based on 1 publication(s) in Google Scholar

Alsterpaullone Related Antibodies

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1 Publications Citing Use of MCE Alsterpaullone

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GSK-3 GSK-3α GSK-3β

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Description

Alsterpaullone (9-Nitropaullone) is a potent CDK inhibitor, with IC50s of 35 nM, 15 nM, 200 nM and 40 nM for CDK1/cyclin B, CDK2/cyclin A, CDK2/cyclin E and CDK5/p35, respectively. Alsterpaullone also competes with ATP for binding to GSK-3alpha/GSK-3beta with IC50s of both 4 nM. Alsterpaullone has antitumor activity, and possesses potential for the study in neurodegenerative and proliferative disorders[1]. Alsterpaullone induces apoptosis in leukemia cell line[2].

IC50 & Target[1]

Cdk1/cyclin B

35 nM (IC50)

cdk2/cyclin A

15 nM (IC50)

CDK2/Cyc E

200 nM (IC50)

CDK5/p35

40 nM (IC50)

GSK-3α

4 (IC50)

GSK-3β

4 nM (IC50)

In Vitro

Alsterpaullone (0.3, 1, 3 μM; 8 hours) induces apoptosis in leukemia cell line[2].
Alsterpaullone (5, 10, 15, 20, 25, 30 μM; 48 and 72 hours) inhibits the growth of HeLa cells in dose-and time-dependent manner. Treatment with Alsterpaullone causes a time-dependent inhibition of cell growth too[3].
Alsterpaullone (20?μM) induces cell death depending on caspase activity[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Alsterpaullone Related Antibodies

Cell Viability Assay[3]

Cell Line: HeLa cells
Concentration: 5, 10, 15, 20, 25, 30 μM
Incubation Time: 48 and 72 hours
Result: The growth of HeLa cells was inhibited in a dose-dependent manner for 48 h and 72 h ranging from 0 to 30 μM.

Apoptosis Analysis[2]

Cell Line: Jurkat cell line
Concentration: 0.3, 1, 3 μM
Incubation Time: 8 hours
Result: Induced dose-dependent apoptosis. 1 μM was sufficient to cause apoptosis, 3 μM demonstrated maximal apoptotic effects[2].

Western Blot Analysis[3]

Cell Line: HeLa cells
Concentration: 2, 4, 6, 12, 24 hours
Incubation Time: 8 hours
Result: The cleavage of PARP started at 4 h, while the activation of caspase-3 occurred at 2 h.
In Vivo

Alsterpaullone (30 mg/kg, s.c., daily for 2 weeks) reduces tumor growth and increases survival in medulloblastoma xenografts[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 5-6 week old athymic nude mice[4]
Dosage: 30 mg/kg
Administration: Subcutaneous injections, daily for 2 weeks
Result: Antitumor effect in Group 3 medulloblastomas.
Molecular Weight

293.28

Formula

C16H11N3O3
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1NC2=CC=CC=C2C(NC3=C4C=C([N+]([O-])=O)C=C3)=C4C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (17.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4097 mL 17.0486 mL 34.0971 mL
5 mM 0.6819 mL 3.4097 mL 6.8194 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.85%

COA (256 KB) LCMS (93 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.4097 mL 17.0486 mL 34.0971 mL 85.2428 mL
5 mM 0.6819 mL 3.4097 mL 6.8194 mL 17.0486 mL
10 mM 0.3410 mL 1.7049 mL 3.4097 mL 8.5243 mL
15 mM 0.2273 mL 1.1366 mL 2.2731 mL 5.6829 mL
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Alsterpaullone Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Alsterpaullone237430-03-49-Nitropaullone NSC 705701NSC705701NSC 705701NSC-705701CDKGSK-3ApoptosisCyclin dependent kinaseGlycogen synthase kinase-3Glycogen synthase kinase 3CDK1cyclin BCDK2cyclin Acyclin ECDK5p35antitumorneurodegenerativeInhibitorinhibitorinhibit

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