AR-C102222 hydrochloride

Name: AR-C102222 hydrochloride
Brand: MedChemExpress
SKU: HY-12122A
Rating: 4.5 (1 reviews)

Cat. No.: HY-12122A Purity: 99.83%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

AR-C102222 hydrochloride is a potent, competitive, orally active and highly selective inducible nitric oxide synthase (iNOS) inhibitor, with an IC₅₀ of 37 nM. AR-C102222 hydrochloride has antinociception and anti-inflammatory activities.

For research use only. We do not sell to patients.

AR-C102222 hydrochloride Chemical Structure

CAS No. : 1781934-50-6

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 308	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 308	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 280	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 450	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 900	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1440	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 2150	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of AR-C102222 hydrochloride:

AR-C102222 Get quote

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nNOS iNOS eNOS

Biological Activity
Purity & Documentation
References
Customer Review

Description

AR-C102222 hydrochloride is a potent, competitive, orally active and highly selective inducible nitric oxide synthase (iNOS) inhibitor, with an IC₅₀ of 37 nM^[1]. AR-C102222 hydrochloride has antinociception and anti-inflammatory activities^[2].

IC₅₀ & Target

iNOS

In Vivo

AR-C102222 (3, 10, 30, 100 mg/kg, P.O.) attenuates arachidonic acid-induced ear inflammation and possesses anti-inflammatory activity^[2].
AR-C102222 shows good efficacy in a rat adjuvant-induced arthritis model^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Balb/c mice (20-25 g)^[2].
Dosage:	3, 10, 30, 100 mg/kg.
Administration:	P.O. 55 min before the administration of 0.6% acetic acid (i.p., 1 mL/100 g volume).
Result:	Significantly inhibited arachidonic acid-induced ear inflammation following a dose of 100 mg/kg of has a maximal inhibition of approximately 79%.

Molecular Weight

418.83

Formula

C₁₉H₁₇ClF₂N₆O

CAS No.

1781934-50-6

Appearance

Solid

Color

White to off-white

SMILES

N#CC1=NC=C(C(N2CCC3(NC4=C(C(F)=CC=C4F)C(N)=N3)CC2)=O)C=C1.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (238.76 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3876 mL	11.9380 mL	23.8760 mL
5 mM	0.4775 mL	2.3876 mL	4.7752 mL
10 mM	0.2388 mL	1.1938 mL	2.3876 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (11.94 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (11.94 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (11.94 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.83%

Select Batch:

Data Sheet (274 KB) SDS (252 KB)

COA (249 KB) HNMR (147 KB) RP-HPLC (164 KB) LCMS (421 KB)

Handling Instructions (2659 KB)

References

[1]. Tinker AC, et al. 1,2-Dihydro-4-quinazolinamines: potent, highly selective inhibitors of inducible nitric oxide synthase which show antiinflammatory activity in vivo. J Med Chem. 2003 Mar 13;46(6):913-6. [Content Brief]

[2]. LaBuda CJ, et al. Antinociceptive activity of the selective iNOS inhibitor AR-C102222 in rodent models of inflammatory, neuropathic and post-operative pain. Eur J Pain. 2006 Aug;10(6):505-12. Epub 2005 Aug 24. [Content Brief]

[3]. Yoon J, et al. Syntheses of 1,2,3-triazolyl salicylamides with inhibitory activity on lipopolysaccharide-induced nitric oxide production. Bioorg Med Chem Lett. 2011 Apr 1;21(7):1953-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3876 mL	11.9380 mL	23.8760 mL	59.6901 mL
	5 mM	0.4775 mL	2.3876 mL	4.7752 mL	11.9380 mL
	10 mM	0.2388 mL	1.1938 mL	2.3876 mL	5.9690 mL
	15 mM	0.1592 mL	0.7959 mL	1.5917 mL	3.9793 mL
	20 mM	0.1194 mL	0.5969 mL	1.1938 mL	2.9845 mL
	25 mM	0.0955 mL	0.4775 mL	0.9550 mL	2.3876 mL
	30 mM	0.0796 mL	0.3979 mL	0.7959 mL	1.9897 mL
	40 mM	0.0597 mL	0.2985 mL	0.5969 mL	1.4923 mL
	50 mM	0.0478 mL	0.2388 mL	0.4775 mL	1.1938 mL
	60 mM	0.0398 mL	0.1990 mL	0.3979 mL	0.9948 mL
	80 mM	0.0298 mL	0.1492 mL	0.2985 mL	0.7461 mL
	100 mM	0.0239 mL	0.1194 mL	0.2388 mL	0.5969 mL