Benzamidine hydrochloride

Name: Benzamidine hydrochloride
Brand: MedChemExpress
SKU: HY-W018781
Rating: 5.0 (2 reviews)

Cat. No.: HY-W018781 Purity: 99.81%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Benzamidine hydrochloride is a trypsin-like serine proteases with K_is of 20, 21, 97, 110, 320 and 750 μM against Tryptase, Trypsin, uPA, Factor Xa, Thrombin and tPA, respectively.

For research use only. We do not sell to patients.

Benzamidine hydrochloride Chemical Structure

CAS No. : 1670-14-0

Size	Price	Stock	Quantity

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 28	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 28	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 25	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
1 g		Get quote
5 g		Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Benzamidine hydrochloride:

Benzamidine hydrochloride hydrate In-stock

2 Publications Citing Use of MCE Benzamidine hydrochloride

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Benzamidine hydrochloride is a trypsin-like serine proteases with K_is of 20, 21, 97, 110, 320 and 750 μM against Tryptase, Trypsin, uPA, Factor Xa, Thrombin and tPA, respectively^[1].

IC₅₀ & Target

Ki: 20 μM (Tryptase), 21 μM (Trypsin), 97 μM (uPA), 110 μM (Factor Xa), 320 μM (Thrombin), 750 μM (tPA)^[1]

In Vitro

Benzamidine hydrochloride (50 μM) reduces [³H]thymidine incorporation in fibroblasts, suggesting dependency on an active catalytic site. Benzamidine hydrochloride reduces the ability of tryptase to stimulate collagen synthesis^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

156.61

Formula

C₇H₉ClN₂

CAS No.

1670-14-0

Appearance

Solid

Color

White to off-white

SMILES

[H]Cl.N=C(C1=CC=CC=C1)N

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (638.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (319.26 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.3853 mL	31.9264 mL	63.8529 mL
5 mM	1.2771 mL	6.3853 mL	12.7706 mL
10 mM	0.6385 mL	3.1926 mL	6.3853 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (15.96 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (15.96 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (15.96 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 110 mg/mL (702.38 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.81%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (250 KB) HNMR (231 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Katz BA, et al. Structural basis for selectivity of a small molecule, S1-binding, submicromolar inhibitor of urokinase-type plasminogen activator. Chem Biol. 2000 Apr;7(4):299-312. [Content Brief]

[2]. J A Cairns, et al. Mast cell tryptase stimulates the synthesis of type I collagen in human lung fibroblasts. J Clin Invest. 1997 Mar 15;99(6):1313-21. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	6.3853 mL	31.9264 mL	63.8529 mL	159.6322 mL
	5 mM	1.2771 mL	6.3853 mL	12.7706 mL	31.9264 mL
	10 mM	0.6385 mL	3.1926 mL	6.3853 mL	15.9632 mL
	15 mM	0.4257 mL	2.1284 mL	4.2569 mL	10.6421 mL
	20 mM	0.3193 mL	1.5963 mL	3.1926 mL	7.9816 mL
	25 mM	0.2554 mL	1.2771 mL	2.5541 mL	6.3853 mL
	30 mM	0.2128 mL	1.0642 mL	2.1284 mL	5.3211 mL
	40 mM	0.1596 mL	0.7982 mL	1.5963 mL	3.9908 mL
	50 mM	0.1277 mL	0.6385 mL	1.2771 mL	3.1926 mL
	60 mM	0.1064 mL	0.5321 mL	1.0642 mL	2.6605 mL
	80 mM	0.0798 mL	0.3991 mL	0.7982 mL	1.9954 mL
	100 mM	0.0639 mL	0.3193 mL	0.6385 mL	1.5963 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.