2. Neuronal Signaling
  3. Monoamine Oxidase
  4. Bifemelane hydrochloride

Bifemelane hydrochloride  (Synonyms: MCI-2016)

Cat. No.: HY-B1558A Purity: 99.86%
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Bifemelane hydrochloride (MCI-2016) is a potent, selective and competitive inhibitor of monoamine oxidase A (MAO-A), with a Ki of 4.20 μM. Bifemelane hydrochloride also inhibits MAO-B noncompetitively with a Ki of 46.0 μM. Bifemelane hydrochloride has a potent antidepressant activity and can be used for the research of cognitive and emotional disturbances related to cerebrovascular disease.

For research use only. We do not sell to patients.

Bifemelane hydrochloride Chemical Structure

Bifemelane hydrochloride Chemical Structure

CAS No. : 62232-46-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 82 In-stock
Solution
10 mM * 1 mL in DMSO USD 82 In-stock
Solid
5 mg USD 75 In-stock
10 mg USD 130 In-stock
25 mg USD 250 In-stock
50 mg USD 400 In-stock
100 mg USD 600 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Bifemelane hydrochloride:

Bifemelane hydrochloride Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Bifemelane hydrochloride

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MAO-A MAO-B

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Bifemelane hydrochloride (MCI-2016) is a potent, selective and competitive inhibitor of monoamine oxidase A (MAO-A), with a Ki of 4.20 μM. Bifemelane hydrochloride also inhibits MAO-B noncompetitively with a Ki of 46.0 μM. Bifemelane hydrochloride has a potent antidepressant activity and can be used for the research of cognitive and emotional disturbances related to cerebrovascular disease[1][2].

IC50 & Target[1]

MAO-A

4.2 μM (Ki)

MAO-B

46 μM (Ki)

In Vitro

Bifemelane inhibits MAO-A in a dose-dependent manner, with Kis of 4.2±0.2 and 14.1±0.7 μM in human brain synaptosomes and human liver mitochondria respectively[1].
Bifemelane inhibits MAO-B activities in a dose-dependent manner, with Kis of 46.0±3.6 and 65.2±7.0 μM in human brain synaptosomes and human liver mitochondria respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Bifemelane hydrochloride Related Antibodies
In Vivo

Bifemelane (20-80 mg/kg; i.p.) dose-dependently decreases exploratory activity in the open field test[3].
Bifemelane (20-80 mg/kg; i.p.) decreases immobility time in the forced swim test, although is not clearly dosedependent and has already reached ceiling at 20 mg/kg[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Wistar male rats (230-270 g) are induced hypothermia by Reserpine[3]
Dosage: 20, 40, 80 mg/kg
Administration: A single i.p.
Result: Attenuated the Reserpine-induced hypothermia by a maximum of 10 ℃.
Molecular Weight

305.84

Formula

C18H24ClNO
CAS No.
Appearance

Solid

Color

White to yellow

SMILES

CNCCCCOC1=CC=CC=C1CC2=CC=CC=C2.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (817.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2697 mL 16.3484 mL 32.6968 mL
5 mM 0.6539 mL 3.2697 mL 6.5394 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (6.80 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (6.80 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2697 mL 16.3484 mL 32.6968 mL 81.7421 mL
5 mM 0.6539 mL 3.2697 mL 6.5394 mL 16.3484 mL
10 mM 0.3270 mL 1.6348 mL 3.2697 mL 8.1742 mL
15 mM 0.2180 mL 1.0899 mL 2.1798 mL 5.4495 mL
20 mM 0.1635 mL 0.8174 mL 1.6348 mL 4.0871 mL
25 mM 0.1308 mL 0.6539 mL 1.3079 mL 3.2697 mL
30 mM 0.1090 mL 0.5449 mL 1.0899 mL 2.7247 mL
40 mM 0.0817 mL 0.4087 mL 0.8174 mL 2.0436 mL
50 mM 0.0654 mL 0.3270 mL 0.6539 mL 1.6348 mL
60 mM 0.0545 mL 0.2725 mL 0.5449 mL 1.3624 mL
80 mM 0.0409 mL 0.2044 mL 0.4087 mL 1.0218 mL
100 mM 0.0327 mL 0.1635 mL 0.3270 mL 0.8174 mL
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Bifemelane hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bifemelane62232-46-6MCI-2016MCI2016MCI 2016Monoamine OxidaseMAOmonoamineoxidaseAMAO-AMAO-BantidepressantInhibitorinhibitorinhibit

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