2. PI3K/Akt/mTOR Metabolic Enzyme/Protease Anti-infection Vitamin D Related/Nuclear Receptor
  3. Drug Metabolite Akt HSV Androgen Receptor Bacterial
  4. Brassicasterol

Brassicasterol is a metabolite of Ergosterol and has cardiovascular protective effects. Brassicasterol exerts anticancer effects in prostate cancer through dual targeting of AKT and androgen receptor signaling pathways. Brassicasterol inhibits HSV-1 (IC50=1.2 μM) and Mycobacterium tuberculosis. Brassicasterol also inhibits sterol δ 24-reductase, slowing the progression of atherosclerosis. Brassicasterol is also a cerebrospinal fluid biomarker for Alzheimer's disease.

For research use only. We do not sell to patients.

Brassicasterol Chemical Structure

Brassicasterol Chemical Structure

CAS No. : 474-67-9

Size Price Stock Quantity
5 mg USD 330 In-stock
10 mg USD 460 In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Brassicasterol Related Antibodies

Top Publications Citing Use of Products

View All Akt Isoform Specific Products:

View All Isoforms
Akt Akt1 Akt2 Akt3

View All HSV Isoform Specific Products:

View All Isoforms
HSV-1 HSV-2 HSV

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Brassicasterol is a metabolite of Ergosterol and has cardiovascular protective effects. Brassicasterol exerts anticancer effects in prostate cancer through dual targeting of AKT and androgen receptor signaling pathways. Brassicasterol inhibits HSV-1 (IC50=1.2 μM) and Mycobacterium tuberculosis. Brassicasterol also inhibits sterol δ 24-reductase, slowing the progression of atherosclerosis. Brassicasterol is also a cerebrospinal fluid biomarker for Alzheimer's disease[1][2][3][4][5][6].

IC50 & Target[2]

HSV-1

1.2 μM (IC50)

In Vitro

Brassicasterol (10 μM; 24 h) downregulates dihydrotestosterone (DHT)-induced expression of androgen receptor (AR) and prostate-specific antigen (PSA) in LNCaP cells [4].
Brassicasterol (50 μM; 48 h) Arrests the cell cycle of LNCaP in the sub-G1 phase and induces cell apoptosis [4].
Brassicasterol (10 μM; 48 h) also inhibits LNCaP cell migration [4].
Brassicasterol (100 μg /mL; 48 h) In a 3D organoid model, it can inhibit AKT, and its inhibitory effect on AR-independent cancers and AR-dependent cell[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Brassicasterol Related Antibodies

Cell Migration Assay [4]

Cell Line: LNCaP cells
Concentration: 0, 10, and 50 μM
Incubation Time: 5 days
Result: Showed 54% cell migration inhibitory effect.
Molecular Weight

398.66

Formula

C28H46O
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

C[C@H](C(C)C)/C=C/[C@@H](C)[C@H]1CC[C@@]2([H])[C@]3([H])CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C
Structure Classification
Initial Source

Hippocampus abdominalis
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

Ethanol : 4.76 mg/mL (11.94 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5084 mL 12.5420 mL 25.0840 mL
5 mM 0.5017 mL 2.5084 mL 5.0168 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% EtOH    90% Corn Oil

    Solubility: ≥ 0.5 mg/mL (1.25 mM); Clear solution

    This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (5.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol 1 mM 2.5084 mL 12.5420 mL 25.0840 mL 62.7101 mL
5 mM 0.5017 mL 2.5084 mL 5.0168 mL 12.5420 mL
10 mM 0.2508 mL 1.2542 mL 2.5084 mL 6.2710 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Brassicasterol Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Brassicasterol474-67-9Drug MetaboliteAktHSVAndrogen ReceptorBacterialPKBProtein kinase BHerpes simplex virusErgosterol metabolitebladder carcinogenesisandrogen signalingHSV-1Mycobacterium tuberculosiscardiovascular protective,AKTprostate cancerInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Brassicasterol
Cat. No.:
HY-113289
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (252 KB) Français - FR (252 KB) Deutsch - DE (252 KB) Norwegian - NO (252 KB) Español - ES (252 KB) Swedish - SV (252 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.