Bromoenol lactone (Synonyms: (6E)-Bromoenol lactone)

Name: Bromoenol lactone
Brand: MedChemExpress
SKU: HY-107411
Rating: 5.0 (2 reviews)

Cat. No.: HY-107411 Purity: 98.41%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Bromoenol lactone ((6E)-Bromoenol lactone) is a suicide-based irreversible, selective, potent inhibitor of calcium-independent phospholipase A₂ (iPLA₂β) with an IC₅₀ value of approximately 7 μM, which inhibits antigen-stimulated mast cell exocytosis without blocking Ca²⁺ influx.

For research use only. We do not sell to patients.

Bromoenol lactone Chemical Structure

CAS No. : 88070-98-8

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 198	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 198	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 116	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 180	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 395	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 695	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1200	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Bromoenol lactone:

2 Publications Citing Use of MCE Bromoenol lactone

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Phospholipase A PLD Phospholipase C

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]^[2]

PLA2

7 μM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
LNCaP	IC₅₀	13 μM Compound: rac-BEL	Antineoplastic activity against human LNCAP cells after 24 hrs by MTT assay Antineoplastic activity against human LNCAP cells after 24 hrs by MTT assay	[PMID: 22677312]
LNCaP	IC₅₀	5 μM Compound: rac-BEL	Antineoplastic activity against human LNCAP cells after 48 hrs by MTT assay Antineoplastic activity against human LNCAP cells after 48 hrs by MTT assay	[PMID: 22677312]
LNCaP	IC₅₀	9 μM Compound: rac-BEL	Antineoplastic activity against human LNCAP cells after 72 hrs by MTT assay Antineoplastic activity against human LNCAP cells after 72 hrs by MTT assay	[PMID: 22677312]
PC-3	IC₅₀	14 μM Compound: rac-BEL	Antineoplastic activity against human PC3 cells after 72 hrs by MTT assay Antineoplastic activity against human PC3 cells after 72 hrs by MTT assay	[PMID: 22677312]
PC-3	IC₅₀	26 μM Compound: rac-BEL	Antineoplastic activity against human PC3 cells after 48 hrs by MTT assay Antineoplastic activity against human PC3 cells after 48 hrs by MTT assay	[PMID: 22677312]
PC-3	IC₅₀	34 μM Compound: rac-BEL	Antineoplastic activity against human PC3 cells after 24 hrs by MTT assay Antineoplastic activity against human PC3 cells after 24 hrs by MTT assay	[PMID: 22677312]

In Vitro

In RBL 2H3 and bone marrow-derived mast cells (BMMCs), Ca²⁺ entry is critical for exocytosis. Bromoenol lactone inhibits exocytosis when stimulated using a Ca²⁺ ionophore A23187, which passively transports Ca²⁺ down a concentration gradient and also in permeabilised mast cells where Ca²⁺ entry is no longer relevant. Moreover, Bromoenol lactone has only a minor effect on antigen- or thapsigargin-stimulated Ca²⁺ signalling, both the release from internal stores and sustained elevation due to Ca²⁺ influx^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

317.18

Formula

C₁₆H₁₃BrO₂

CAS No.

88070-98-8

Appearance

Solid

Color

White to yellow

SMILES

O=C1C(C2=C3C=CC=CC3=CC=C2)CC/C(O1)=C\Br

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (157.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1528 mL	15.7639 mL	31.5278 mL
5 mM	0.6306 mL	3.1528 mL	6.3056 mL
10 mM	0.3153 mL	1.5764 mL	3.1528 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (7.88 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (7.88 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.41%

Select Batch:

Data Sheet (276 KB) SDS (252 KB)

COA (251 KB) RP-HPLC (246 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Fensome-Green A, et al. Bromoenol lactone, an inhibitor of Group V1A calcium-independent phospholipase A2 inhibits antigen-stimulated mast cell exocytosis without blocking Ca2+ influx. Cell Calcium. 2007 Feb;41(2):145-53. [Content Brief]

[2]. Takuma T, et al. Role of Ca2+-independent phospholipase A2 in exocytosis of amylase from parotid acinar cells. J Biochem. 1997 Jun;121(6):1018-24. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.1528 mL	15.7639 mL	31.5278 mL	78.8196 mL
	5 mM	0.6306 mL	3.1528 mL	6.3056 mL	15.7639 mL
	10 mM	0.3153 mL	1.5764 mL	3.1528 mL	7.8820 mL
	15 mM	0.2102 mL	1.0509 mL	2.1019 mL	5.2546 mL
	20 mM	0.1576 mL	0.7882 mL	1.5764 mL	3.9410 mL
	25 mM	0.1261 mL	0.6306 mL	1.2611 mL	3.1528 mL
	30 mM	0.1051 mL	0.5255 mL	1.0509 mL	2.6273 mL
	40 mM	0.0788 mL	0.3941 mL	0.7882 mL	1.9705 mL
	50 mM	0.0631 mL	0.3153 mL	0.6306 mL	1.5764 mL
	60 mM	0.0525 mL	0.2627 mL	0.5255 mL	1.3137 mL
	80 mM	0.0394 mL	0.1970 mL	0.3941 mL	0.9852 mL
	100 mM	0.0315 mL	0.1576 mL	0.3153 mL	0.7882 mL

Bromoenol lactone Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bromoenol lactone88070-98-8(6E)-Bromoenol lactonePhospholipaseInhibitorinhibitorinhibit

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Bromoenol lactone (Synonyms: (6E)-Bromoenol lactone)

Customer Review

Other Forms of Bromoenol lactone:

Bromoenol lactone Related Antibodies

2 Publications Citing Use of MCE Bromoenol lactone

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Phospholipase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Bromoenol lactone Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Bromoenol lactone Related Classifications

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