2. Autophagy Membrane Transporter/Ion Channel
  3. Autophagy Calmodulin
  4. Calmidazolium chloride

Calmidazolium chloride  (Synonyms: R 24571)

Cat. No.: HY-103319 Purity: 99.69%
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Calmidazolium chloride (R 24571) is a calmodulin antagonist, antagonizing CaM-dependent phosphodiesterase and calmodulin-induced activation of erythrocyte Ca2+-transporting ATPase with IC50s of 0.15 and 0.35 μM, respectively. Also in anti-cancer research. Calmidazolium binds to calmodulin with a Kd of 3 nM.

For research use only. We do not sell to patients.

Calmidazolium chloride Chemical Structure

Calmidazolium chloride Chemical Structure

CAS No. : 57265-65-3

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10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

Calmidazolium chloride Related Antibodies

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Description

Calmidazolium chloride (R 24571) is a calmodulin antagonist, antagonizing CaM-dependent phosphodiesterase and calmodulin-induced activation of erythrocyte Ca2+-transporting ATPase with IC50s of 0.15 and 0.35 μM, respectively[1]. Also in anti-cancer research[2]. Calmidazolium binds to calmodulin with a Kd of 3 nM.

IC50 & Target

Kd: 3 nM (Calmodulin)[3]
In Vitro

Calmidazolium chloride is widely used as a calmodulin (CaM) antagonist, but is also known to induce apoptosis in certain cancer cell lines. Calmidazolium chloride (3, 5, 7, 10 μM, 30 minutes-24 hours) inhibits growth of mouse F9 ECCs[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Calmidazolium chloride Related Antibodies

Cell Viability Assay[2]

Cell Line: Mouse F9 ECCs
Concentration: 3, 5, 7, 10 μM
Incubation Time: 30 minutes-24 hours
Result: The IC50s of Calmidazolium chloride treated F9 ECCs and E14 ESCs are 8.18 μM, and 12.69 μM[2].
Molecular Weight

687.70

Formula

C31H23Cl7N2O
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

ClC1=CC=C(COC(C2=CC=C(Cl)C=C2Cl)CN3C=C[N+](C(C4=CC=C(Cl)C=C4)C5=CC=C(Cl)C=C5)=C3)C(Cl)=C1.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (145.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4541 mL 7.2706 mL 14.5412 mL
5 mM 0.2908 mL 1.4541 mL 2.9082 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (3.02 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (3.02 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.69%

SDS (393 KB)

COA (255 KB) LCMS (256 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4541 mL 7.2706 mL 14.5412 mL 36.3531 mL
5 mM 0.2908 mL 1.4541 mL 2.9082 mL 7.2706 mL
10 mM 0.1454 mL 0.7271 mL 1.4541 mL 3.6353 mL
15 mM 0.0969 mL 0.4847 mL 0.9694 mL 2.4235 mL
20 mM 0.0727 mL 0.3635 mL 0.7271 mL 1.8177 mL
25 mM 0.0582 mL 0.2908 mL 0.5816 mL 1.4541 mL
30 mM 0.0485 mL 0.2424 mL 0.4847 mL 1.2118 mL
40 mM 0.0364 mL 0.1818 mL 0.3635 mL 0.9088 mL
50 mM 0.0291 mL 0.1454 mL 0.2908 mL 0.7271 mL
60 mM 0.0242 mL 0.1212 mL 0.2424 mL 0.6059 mL
80 mM 0.0182 mL 0.0909 mL 0.1818 mL 0.4544 mL
100 mM 0.0145 mL 0.0727 mL 0.1454 mL 0.3635 mL
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Calmidazolium chloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Calmidazolium57265-65-3R 24571R24571R-24571AutophagyCalmodulinCaMCalcium-modulated proteinInhibitorinhibitorinhibit

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