Caspase-3-IN-1

Name: Caspase-3-IN-1
Brand: MedChemExpress
SKU: HY-136975
Rating: 4.5 (1 reviews)

Cat. No.: HY-136975 Purity: 97.04%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Caspase-3-IN-1 (compound 11b) is a potent Caspase-3 inhibitor, with an IC₅₀ of 14.5 nM.

For research use only. We do not sell to patients.

Caspase-3-IN-1 Chemical Structure

CAS No. : 872254-32-5

Size	Price	Stock	Quantity
5 mg	USD 230	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 360	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 680	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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View All Isoforms

Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

Biological Activity
Purity & Documentation
References
Customer Review

Description

Caspase-3-IN-1 (compound 11b) is a potent Caspase-3 inhibitor, with an IC₅₀ of 14.5 nM^[1].

IC₅₀ & Target

Caspase-3

14.5 ± 1.6 nM (IC₅₀)

Caspase-7

21.8 ± 3.5 nM (IC₅₀)

Caspase-6

>5000 nM (IC₅₀)

Caspase-1

>10000 nM (IC₅₀)

Caspase-8

>50000 nM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	EC₅₀	0.45 μM Compound: 58	Inhibition of staurosporine induced activation of caspase 3 activation in human Hela cells assessed as hydrolysis of Z-DEVD-R110 substrate by microplate fluorescence assay Inhibition of staurosporine induced activation of caspase 3 activation in human Hela cells assessed as hydrolysis of Z-DEVD-R110 substrate by microplate fluorescence assay	[PMID: 21441025]

Molecular Weight

507.56

Formula

C₂₆H₂₅N₃O₆S

CAS No.

872254-32-5

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=S(C1=CC=C(C(C(C2=O)=O)=C1)N2CC3=CC=C(C=C3)OC)(N4[C@@H](CCC4)COC5=CN=CC=C5)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (197.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9702 mL	9.8511 mL	19.7021 mL
5 mM	0.3940 mL	1.9702 mL	3.9404 mL
10 mM	0.1970 mL	0.9851 mL	1.9702 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (4.93 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 97.04%

Select Batch:

Data Sheet (276 KB) SDS (251 KB)

COA (256 KB) HNMR (261 KB) RP-HPLC (197 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Chu W, et al. N-benzylisatin sulfonamide analogues as potent caspase-3 inhibitors: synthesis, in vitro activity, and molecular modeling studies. J Med Chem. 2005 Dec 1;48(24):7637-47. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9702 mL	9.8511 mL	19.7021 mL	49.2553 mL
	5 mM	0.3940 mL	1.9702 mL	3.9404 mL	9.8511 mL
	10 mM	0.1970 mL	0.9851 mL	1.9702 mL	4.9255 mL
	15 mM	0.1313 mL	0.6567 mL	1.3135 mL	3.2837 mL
	20 mM	0.0985 mL	0.4926 mL	0.9851 mL	2.4628 mL
	25 mM	0.0788 mL	0.3940 mL	0.7881 mL	1.9702 mL
	30 mM	0.0657 mL	0.3284 mL	0.6567 mL	1.6418 mL
	40 mM	0.0493 mL	0.2463 mL	0.4926 mL	1.2314 mL
	50 mM	0.0394 mL	0.1970 mL	0.3940 mL	0.9851 mL
	60 mM	0.0328 mL	0.1642 mL	0.3284 mL	0.8209 mL
	80 mM	0.0246 mL	0.1231 mL	0.2463 mL	0.6157 mL
	100 mM	0.0197 mL	0.0985 mL	0.1970 mL	0.4926 mL

Caspase-3-IN-1 Related Classifications

Neurological Disease Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Caspase-3-IN-1872254-32-5Caspasepyrrolidine analoguescaspaseapoptosisInhibitorinhibitorinhibit

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Caspase-3-IN-1

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Caspase-3-IN-1 Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

Caspase-3-IN-1 Related Classifications

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