2. Apoptosis
  3. Apoptosis Necroptosis
  4. Cyclamic acid

Cyclamic acid  (Synonyms: Cyclohexylsulfamic acid; Cyclamate)

Cat. No.: HY-B0541 Purity: ≥98.0%
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Cyclamic acid (Cyclamate) is a commonly used sweetener. Cyclamic acid sodium is toxic to osteoblasts and can inhibit cell proliferation, induce apoptosis and reduce cell mineralization. Cyclamic acid sodium causes focal necrosis of bladder organs in rats in vitro, which can promote bladder cancer, but some studies have shown that low doses of Cyclamic acid sodium have no carcinogenic effect. In addition, Cyclamic acid sodium has no effect on insulin and glucagon secretion induced by arginine.

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Cyclamic acid Chemical Structure

Cyclamic acid Chemical Structure

CAS No. : 100-88-9

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Based on 1 publication(s) in Google Scholar

Other Forms of Cyclamic acid:

Cyclamic acid Related Antibodies

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Description

Cyclamic acid (Cyclamate) is a commonly used sweetener. Cyclamic acid sodium is toxic to osteoblasts and can inhibit cell proliferation, induce apoptosis and reduce cell mineralization. Cyclamic acid sodium causes focal necrosis of bladder organs in rats in vitro, which can promote bladder cancer, but some studies have shown that low doses of Cyclamic acid sodium have no carcinogenic effect. In addition, Cyclamic acid sodium has no effect on insulin and glucagon secretion induced by arginine[1][2][3][4][5].

In Vitro

Cyclamic acid (0-0.1 μM, 24-72 h) causes the apoptosis of osteoblast cells and shows significant inhibition of osteoblasts[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cyclamic acid Related Antibodies

Cell Viability Assay[2]

Cell Line: Osteoblast cells
Concentration: 0-0.1 μM
Incubation Time: 24-72 h
Result: Resulted death of more than 50% osteoblasts after 72 h's treatment.

Western Blot Analysis[2]

Cell Line: Osteoblast cells
Concentration: 0-0.1 μM
Incubation Time: 24-72 h
Result: Decreased the expression of BMP2 and reduced the mineralization capacity of osteoblasts by lowing the expression of ALP.
In Vivo

Cyclamic acid (2-4 g/kg, p.o., daily for 2 weeks) occurs diarrhea in dogs[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Dogs[3]
Dosage: 2-4 g/kg
Administration: p.o., daily for 2 weeks
Result: Occurred diarrhea in dogs.
Molecular Weight

179.24

Formula

C6H13NO3S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=S(O)(NC1CCCCC1)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL (557.91 mM)

DMSO : 100 mg/mL (557.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.5791 mL 27.8956 mL 55.7911 mL
5 mM 1.1158 mL 5.5791 mL 11.1582 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (13.95 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (13.95 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 5.5791 mL 27.8956 mL 55.7911 mL 139.4778 mL
5 mM 1.1158 mL 5.5791 mL 11.1582 mL 27.8956 mL
10 mM 0.5579 mL 2.7896 mL 5.5791 mL 13.9478 mL
15 mM 0.3719 mL 1.8597 mL 3.7194 mL 9.2985 mL
20 mM 0.2790 mL 1.3948 mL 2.7896 mL 6.9739 mL
25 mM 0.2232 mL 1.1158 mL 2.2316 mL 5.5791 mL
30 mM 0.1860 mL 0.9299 mL 1.8597 mL 4.6493 mL
40 mM 0.1395 mL 0.6974 mL 1.3948 mL 3.4869 mL
50 mM 0.1116 mL 0.5579 mL 1.1158 mL 2.7896 mL
60 mM 0.0930 mL 0.4649 mL 0.9299 mL 2.3246 mL
80 mM 0.0697 mL 0.3487 mL 0.6974 mL 1.7435 mL
100 mM 0.0558 mL 0.2790 mL 0.5579 mL 1.3948 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Cyclamic acid Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cyclamic acid100-88-9Cyclohexylsulfamic acid CyclamateApoptosisNecroptosisartificialsweetenerInhibitorinhibitorinhibit

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