DC-Chol hydrochloride (Synonyms: DC-Cholesterol hydrochloride)

Name: DC-Chol hydrochloride
Brand: MedChemExpress
SKU: HY-137131
Rating: 4.5 (1 reviews)

Cat. No.: HY-137131 Purity: 99.91%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

DC-Chol hydrochloride is a cationic lipid. DC-Chol hydrochloride could inhibit Aβ40 fibril formation under appropriate experimental conditions. DC-Chol hydrochloride strongly inhibits amyloidogenesis of oxidized hCT in a dose-dependent manner. DC-Chol hydrochloride induces the production of Th1 (IL-2 and IFN-γ) and Th2 (IL-5) cytokines. DC-Chol hydrochloride can enhance the body's immune response to antigens. DC-Chol hydrochloride is used as a gene delivery vector. DC-Chol hydrochloride can be used in research in areas such as hepatitis B vaccines to improve vaccine immunity.

For research use only. We do not sell to patients.

DC-Chol hydrochloride Chemical Structure

CAS No. : 166023-21-8

Size	Stock	Quantity
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of DC-Chol hydrochloride:

3β-[N-(N′,N′-Dimethylaminoethyl)carbamoyl]cholesterol Get quote

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IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

DC-Chol (hydrochloride) enhances the in vitro proliferative response of lymphocytes from mice immunized with HBsAg and induces the production of Th1 (IL-2 and IFN-γ) and Th2 (IL-5) cytokines^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

DC-Chol (0.5 mg; s.c.; twice; 3-week interval between injections) hydrochloride increases anti - HBsAg antibody levels, induces both Th1 and Th2 immune responses, and overcomes unresponsiveness to HBsAg in non-responder mice strains^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	BALB/c mice (female, 6-8-week-old), OF1 mice (female, 6-8-week-old), B10.M mice (female, 6-8-week-old); mouse models for studying immune response to HBsAg vaccine^[3]
Dosage:	0.5 mg DC-Chol in Tris/HCl 20 mM, NaCl 150 mM, pH 6.8 for all mouse models
Administration:	Subcutaneous injection, twice, with a 3-week interval between injections for all mouse models
Result:	Increased anti-HBsAg IgG antibody titres, induced higher levels of both IgG1 and IgG2a antibodies, and enhanced cellular immune responses in BALB/c mice. Induced a higher seroconversion rate and higher antibody titres compared to alum-adjuvanted vaccine in OF1 mice. Induced a seroconversion rate of 63% in B10.M mice (non-responders to HBsAg).

Molecular Weight

537.26

Formula

C₃₂H₅₇ClN₂O₂

CAS No.

166023-21-8

Appearance

Solid

Color

White to light yellow

SMILES

CC(C)CCC[C@@H](C)[C@H]1CC[C@@]2([H])[C@]3([H])CC=C4C[C@@H](OC(NCCN(C)C)=O)CC[C@]4(C)[C@@]3([H])CC[C@]12C.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

Ethanol : 12.5 mg/mL (23.27 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8613 mL	9.3065 mL	18.6130 mL
5 mM	0.3723 mL	1.8613 mL	3.7226 mL
10 mM	0.1861 mL	0.9306 mL	1.8613 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.33 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (2.33 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 1.25 mg/mL (2.33 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (247 KB) HNMR (304 KB) RP-HPLC (241 KB) MS (178 KB)

Handling Instructions (2659 KB)

References

[1]. Esmail A Elbassal, et al. Effects of Charged Cholesterol Derivatives on Aβ40 Amyloid Formation. J Phys Chem B. 2016 Jan 14;120(1):59-68. [Content Brief]

[2]. Richard Lantz, et al. Effects of disulfide bond and cholesterol derivatives on human calcitonin amyloid formation. Biopolymers. 2020 May;111(5):e23343. [Content Brief]

[3]. Brunel F, et al. Cationic lipid DC-Chol induces an improved and balanced immunity able to overcome the unresponsiveness to the hepatitis B vaccine. Vaccine. 1999 Apr 23;17(17):2192-203. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol	1 mM	1.8613 mL	9.3065 mL	18.6130 mL	46.5324 mL
	5 mM	0.3723 mL	1.8613 mL	3.7226 mL	9.3065 mL
	10 mM	0.1861 mL	0.9306 mL	1.8613 mL	4.6532 mL
	15 mM	0.1241 mL	0.6204 mL	1.2409 mL	3.1022 mL
	20 mM	0.0931 mL	0.4653 mL	0.9306 mL	2.3266 mL

DC-Chol hydrochloride Related Classifications

Neurological Disease Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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DC-Chol hydrochloride (Synonyms: DC-Cholesterol hydrochloride)

Customer Review

Other Forms of DC-Chol hydrochloride:

DC-Chol hydrochloride Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Interleukin Related Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

DC-Chol hydrochloride Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

DC-Chol hydrochloride Related Classifications

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SAFETY DATA SHEET (SDS)

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