2. NF-κB Immunology/Inflammation
  3. NF-κB COX PGE synthase NO Synthase
  4. Dehydroevodiamine

Dehydroevodiamine is a major bioactive quinazoline alkaloid isolated from Evodiae Fructus, has an antiarrhythmic effect in guinea-pig ventricular myocytes. Dehydroevodiamine inhibits LPS-induced iNOS, COX-2, prostaglandin E2 (PGE2) and nuclear factor-kappa B (NF-κB) expression in murine macrophage cells.

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Dehydroevodiamine Chemical Structure

Dehydroevodiamine Chemical Structure

CAS No. : 67909-49-3

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10 mg USD 60 In-stock
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Other Forms of Dehydroevodiamine:

Dehydroevodiamine Related Antibodies

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

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COX COX-1 COX-2 COX-3

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nNOS iNOS eNOS

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Dehydroevodiamine is a major bioactive quinazoline alkaloid isolated from Evodiae Fructus, has an antiarrhythmic effect in guinea-pig ventricular myocytes[1]. Dehydroevodiamine inhibits LPS-induced iNOS, COX-2, prostaglandin E2 (PGE2) and nuclear factor-kappa B (NF-κB) expression in murine macrophage cells[2].

IC50 & Target

iNOS

 

In Vitro

Dehydroevodiamine (0-50 μM; 2 hours) inhibits iNOS and COX-2 expression and prevents degradation of IκB-α in LPS induced RAW 264.7 macrophages[2].
Dehydroevodiamine (0-50 μM; 2 hours) inhibits a LPS-induced increase in the iNOS and COX-2 mRNA expression[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Dehydroevodiamine Related Antibodies

Western Blot Analysis[2]

Cell Line: RAW 264.7 macrophage cells
Concentration: 10 μM, 30 μM, 50 μM
Incubation Time: pretreated 2 hours
Result: Reduced iNOS and COX-2 expression and increased IκB-α expression.

RT-PCR[2]

Cell Line: RAW 264.7 macrophage cells
Concentration: 10 μM, 30 μM, 50 μM
Incubation Time: pretreated 2 hours
Result: Reduced iNOS and COX-2 mRNA expression.
Molecular Weight

301.34

Formula

C19H15N3O
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1N2C(C([N-]C3=C4C=CC=C3)=C4CC2)=[N+](C)C5=C1C=CC=C5
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 8.33 mg/mL (27.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3185 mL 16.5926 mL 33.1851 mL
5 mM 0.6637 mL 3.3185 mL 6.6370 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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Volume (start)

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Concentration (final)

C2

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In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  0.5% CMC-Na/saline water

    Solubility: 10 mg/mL (33.19 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3185 mL 16.5926 mL 33.1851 mL 82.9628 mL
5 mM 0.6637 mL 3.3185 mL 6.6370 mL 16.5926 mL
10 mM 0.3319 mL 1.6593 mL 3.3185 mL 8.2963 mL
15 mM 0.2212 mL 1.1062 mL 2.2123 mL 5.5309 mL
20 mM 0.1659 mL 0.8296 mL 1.6593 mL 4.1481 mL
25 mM 0.1327 mL 0.6637 mL 1.3274 mL 3.3185 mL
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Dehydroevodiamine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dehydroevodiamine67909-49-3NF-κBCOXPGE synthaseNO SynthaseNuclear factor-κBNuclear factor-kappaBCyclooxygenaseProstaglandin E synthaseNitric oxide synthasesNOSInhibitorinhibitorinhibit

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