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  6. Diallyl disulfide

Diallyl disulfide, an active compound in garlic oil, is an orally active human squalene monooxygenase inhibitor with an IC50 of 400 μM for squalene epoxidation. Diallyl disulfide exhibits obvious anti-inflammatory, anti-oxidative, antidepressant and anti-tumor activities.

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Diallyl disulfide Chemical Structure

Diallyl disulfide Chemical Structure

CAS No. : 2179-57-9

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Based on 1 publication(s) in Google Scholar

Other Forms of Diallyl disulfide:

Diallyl disulfide Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Diallyl disulfide

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Diallyl disulfide, an active compound in garlic oil, is an orally active human squalene monooxygenase inhibitor with an IC50 of 400 μM for squalene epoxidation. Diallyl disulfide exhibits obvious anti-inflammatory, anti-oxidative, antidepressant and anti-tumor activities[1][2][3][4].

In Vitro

Diallyl disulfide (30, 60, 120, 240 μM; 12, 24, 48 h) suppresses HT-29 cell growth[2].
Diallyl disulfide (30, 60, 120 μM; 24 h) induces cell cycle arrest in the G2/M-phase in HT-29 cells in a concentration-independent manner[2].
Diallyl disulfide (120 μM) induces the mRNA levels of ARPC3, SPINT2, SOD1, p21 and TACSTD2[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Diallyl disulfide Related Antibodies

Cell Viability Assay[2]

Cell Line: HT-29 cells
Concentration: 30, 60, 120 and 240 μM
Incubation Time: 12, 24, 48 h
Result: Suppressed HT-29 cell growth by 5.3, 23.1, 45.6 and 68.3% with 30, 60, 120 and 240 µM for 24 h, respectively.

Cell Cycle Analysis[2]

Cell Line: HT-29 cells
Concentration: 30, 60, 120 μM
Incubation Time: 24 h
Result: The percentage of cells in the G2/M-phase was found to be 23.6, 25.9, 39.7 and 61.0% with 30, 60, 120 and 240 µM, respectively.

RT-PCR[2]

Cell Line: HT-29 cells
Concentration: 120 μM
Incubation Time:
Result: The mRNA levels of ARPC3, SPINT2, SOD1, p21 and TACSTD2 were 3.52-, 7.98-, 15.6-, 13.47- and 5.92-fold higher than those in the primary cultured cells, respectively.
In Vivo

Diallyl disulfide (40, 80 mg/kg; IP; pre-treatment 1 h) has a prophylactic effect in LPS-induced depression-like behaviors in mice[3].
Diallyl disulfide (30, 60, 90 mg/kg; oral gavage; three times/week for the last 30 days) dose-dependently reduces cigarette smoke (CS) -induced leukocyte infiltration into the airways and improves lung histology in C57BL/6 mice were exposed to CS for 60 consecutive days[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male C57BL6/J mice (6-8 weeks)[3]
Dosage: 40, 80 mg/kg
Administration: IP; pre-treatment 1 h before a single LPS injection (100 μg/kg)
Result: Prevented LPS-induced increases in immobility time in the TST and FST in mice.
Did not affect the locomotor activity in mice with or without LPS treatment.
Molecular Weight

146.27

Formula

C6H10S2
CAS No.
Appearance

Liquid (Density: 1.010 g/cm3)

Color

Colorless to light yellow

SMILES

C=CCSSCC=C
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (683.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.8367 mL 34.1834 mL 68.3667 mL
5 mM 1.3673 mL 6.8367 mL 13.6733 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (17.09 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (17.09 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.36%

COA (242 KB) HNMR (238 KB) LCMS (268 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 6.8367 mL 34.1834 mL 68.3667 mL 170.9168 mL
5 mM 1.3673 mL 6.8367 mL 13.6733 mL 34.1834 mL
10 mM 0.6837 mL 3.4183 mL 6.8367 mL 17.0917 mL
15 mM 0.4558 mL 2.2789 mL 4.5578 mL 11.3945 mL
20 mM 0.3418 mL 1.7092 mL 3.4183 mL 8.5458 mL
25 mM 0.2735 mL 1.3673 mL 2.7347 mL 6.8367 mL
30 mM 0.2279 mL 1.1394 mL 2.2789 mL 5.6972 mL
40 mM 0.1709 mL 0.8546 mL 1.7092 mL 4.2729 mL
50 mM 0.1367 mL 0.6837 mL 1.3673 mL 3.4183 mL
60 mM 0.1139 mL 0.5697 mL 1.1394 mL 2.8486 mL
80 mM 0.0855 mL 0.4273 mL 0.8546 mL 2.1365 mL
100 mM 0.0684 mL 0.3418 mL 0.6837 mL 1.7092 mL
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Diallyl disulfide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Diallyl disulfide2179-57-9Othersgarlic oilsqualene monooxygenasesqualene epoxidationanti-inflammatoryanti-oxidativeantidepressantanti-tumorInhibitorinhibitorinhibit

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