2. Epigenetics
  3. DNA Methyltransferase
  4. DY-46-2

DY-46-2 is a high potency and selectivity novel non-nucleoside DNA methyltransferase 3A (DNMT3A) inhibitor with an IC50 value of 0.39 μM.

For research use only. We do not sell to patients.

DY-46-2 Chemical Structure

DY-46-2 Chemical Structure

CAS No. : 1105110-83-5

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 495 In-stock
Solution
10 mM * 1 mL in DMSO USD 495 In-stock
Solid
1 mg USD 180 In-stock
5 mg USD 450 In-stock
10 mg USD 700 In-stock
25 mg USD 1480 In-stock
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Based on 1 publication(s) in Google Scholar

DY-46-2 Related Antibodies

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Description

DY-46-2 is a high potency and selectivity novel non-nucleoside DNA methyltransferase 3A (DNMT3A) inhibitor with an IC50 value of 0.39 μM[1].

IC50 & Target

DNMT3A

0.39 μM (IC50)

In Vitro

DY-46-2 (0.1-100 μM, 24, 48 and 72 h) has an inhibitory activity of DNMT3A with an IC50 value of 0.39 μM, which increases linearly with DNA concentration (IDT-01)[1].
DY-46-2 (0.1-100 μM, 24, 48 and 72 h) has inhibitory activity against DNMT1, DNMT3B and G9a with IC50 values of 13.0 μM, 105 μM and ＞500 μM, respectively[1].
DY-46-2 (0.1-100 μM, 24, 48 and 72 h) has cell viability in cancer cells with IC50 values of 0.7 μM, 0.3 μM, 0.7 μM, 0.5 μM, 2.1 μM, 1.7 μM and 91 μM for THP-1, HCT116, U937, K562, A549, DU145 and PBMC cell, respectively[1].
DY-46-2 (0.1-100 μM, 24, 48 and 72 h) markedly inhibits the proliferation of cancer cells and shows low cytotoxicity in peripheral blood mononuclear cells (PBMCs)[1].
DY-46-2 (1 μM, 72 h) obviously decreases DNMT3A protein levels, as well as reactive expression of a silenced TSG (p53) in HCT116 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DY-46-2 Related Antibodies

Cell Viability Assay[1]

Cell Line: THP-1, HCT116, U937, K562, A549, DU145 and PBMC cell
Concentration: 0.1-100 μM
Incubation Time: 24, 48 and 72 h
Result: Had remarkable inhibitory potency in the dose- and time-dependent manners and no cytotoxicity in non-tumoral PBMCs (IC50 >100 μM).

Cell Proliferation Assay[1]

Cell Line: THP-1, HCT116, U937, K562, A549, DU145 and PBMC cell
Concentration: 0.1-100 μM
Incubation Time: 24, 48 and 72 h
Result: Exhibited high anti-proliferative activity with a micromolar range cytotoxicity in all cancer cells.

Western Blot Analysis[1]

Cell Line: HCT116 cells
Concentration: 1 μM
Incubation Time: 72 h
Result: Decreased DNMT3A and p53 protein levels in the HCT116 cells, apparently sufficient to reactive expression of a silenced TSG (p53).
Molecular Weight

446.48

Formula

C19H22N6O5S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=C(C)OC(N=CN2CCN3CCCC3)=C1C2=O)NC4=CC=C(S(=O)(N)=O)N=C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (111.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2397 mL 11.1987 mL 22.3974 mL
5 mM 0.4479 mL 2.2397 mL 4.4795 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.32%

COA (254 KB) HNMR (146 KB) RP-HPLC (168 KB) LCMS (379 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2397 mL 11.1987 mL 22.3974 mL 55.9935 mL
5 mM 0.4479 mL 2.2397 mL 4.4795 mL 11.1987 mL
10 mM 0.2240 mL 1.1199 mL 2.2397 mL 5.5994 mL
15 mM 0.1493 mL 0.7466 mL 1.4932 mL 3.7329 mL
20 mM 0.1120 mL 0.5599 mL 1.1199 mL 2.7997 mL
25 mM 0.0896 mL 0.4479 mL 0.8959 mL 2.2397 mL
30 mM 0.0747 mL 0.3733 mL 0.7466 mL 1.8665 mL
40 mM 0.0560 mL 0.2800 mL 0.5599 mL 1.3998 mL
50 mM 0.0448 mL 0.2240 mL 0.4479 mL 1.1199 mL
60 mM 0.0373 mL 0.1866 mL 0.3733 mL 0.9332 mL
80 mM 0.0280 mL 0.1400 mL 0.2800 mL 0.6999 mL
100 mM 0.0224 mL 0.1120 mL 0.2240 mL 0.5599 mL
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DY-46-2 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DY-46-21105110-83-5DNA MethyltransferaseDNMTsDNA MTasesDNA methyltransferase 3A (DNMT3A)cancerInhibitorinhibitorinhibit

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