2. Cell Cycle/DNA Damage Autophagy
  3. Eukaryotic Initiation Factor (eIF) Autophagy
  4. eIF4A3-IN-2

eIF4A3-IN-2 est un inhibiteur de facteur d'initiation eucaryote 4b-3 (eIF4A3) qui est hautement sélectif et non compétitif avec un IC50 de 110 nM.

eIF4A3-IN-2 is a highly selective and noncompetitive eukaryotic initiation factor 4A-3 (eIF4A3) inhibitor with an IC50 of 110 nM.

For research use only. We do not sell to patients.

eIF4A3-IN-2 Chemical Structure

eIF4A3-IN-2 Chemical Structure

CAS No. : 2095677-20-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 729 In-stock
Solution
10 mM * 1 mL in DMSO USD 729 In-stock
Solid
1 mg USD 220 In-stock
5 mg USD 550 In-stock
10 mg USD 850 In-stock
50 mg USD 2650 In-stock
100 mg USD 3950 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of eIF4A3-IN-2:

eIF4A3-IN-2 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE eIF4A3-IN-2

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

eIF4A3-IN-2 is a highly selective and noncompetitive eukaryotic initiation factor 4A-3 (eIF4A3) inhibitor with an IC50 of 110 nM[1].

IC50 & Target

eIF4

 

In Vitro

eIF4A3-IN-2 (compound 2) binds to the allosteric region in eIF4A3 and inhibits in vitro ATPase, helicase, and cellular nonsense-mediated RNA decay (NMD) activities[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

eIF4A3-IN-2 Related Antibodies

Apoptosis Analysis[1]

Cell Line: HEK293T cells (transfected with the NMD reporter) luciferase assay
Concentration: 0.3, 1, 3, or 10 μM
Incubation Time: 3 or 6 h
Result: eIF4A3-IN-2 induces an approximately 3.2-fold increase in luciferase activity, indicating that NMD is inhibited by compound 2.
Molecular Weight

602.70

Formula

C25H19Br2ClN4O2
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O=C(C1=CC=C(Br)C=C1)N2CCN(C(C3=C4N(C=C(Br)C=C4)N=C3)=O)C[C@@H]2C5=CC=C(Cl)C=C5
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (165.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6592 mL 8.2960 mL 16.5920 mL
5 mM 0.3318 mL 1.6592 mL 3.3184 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  5% DMSO    40% PEG300    5% Tween-80    50% Saline

    Solubility: 2.5 mg/mL (4.15 mM); Suspended solution; Need ultrasonic

  • Protocol 2

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.67 mg/mL (2.77 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.77%

COA (257 KB) LCMS (272 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6592 mL 8.2960 mL 16.5920 mL 41.4800 mL
5 mM 0.3318 mL 1.6592 mL 3.3184 mL 8.2960 mL
10 mM 0.1659 mL 0.8296 mL 1.6592 mL 4.1480 mL
15 mM 0.1106 mL 0.5531 mL 1.1061 mL 2.7653 mL
20 mM 0.0830 mL 0.4148 mL 0.8296 mL 2.0740 mL
25 mM 0.0664 mL 0.3318 mL 0.6637 mL 1.6592 mL
30 mM 0.0553 mL 0.2765 mL 0.5531 mL 1.3827 mL
40 mM 0.0415 mL 0.2074 mL 0.4148 mL 1.0370 mL
50 mM 0.0332 mL 0.1659 mL 0.3318 mL 0.8296 mL
60 mM 0.0277 mL 0.1383 mL 0.2765 mL 0.6913 mL
80 mM 0.0207 mL 0.1037 mL 0.2074 mL 0.5185 mL
100 mM 0.0166 mL 0.0830 mL 0.1659 mL 0.4148 mL
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eIF4A3-IN-2 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

eIF4A3-IN-22095677-20-4Eukaryotic Initiation Factor (eIF)AutophagyInhibitorinhibitorinhibit

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