eIF4A3-IN-2

Name: eIF4A3-IN-2
Brand: MedChemExpress
SKU: HY-101785
Rating: 4.5 (1 reviews)

Cat. No.: HY-101785 Purity: 99.70%: FAQs
Data Sheet SDS COA Handling Instructions

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eIF4A3-IN-2 is a highly selective and noncompetitive eukaryotic initiation factor 4A-3 (eIF4A3) inhibitor with an IC₅₀ of 110 nM.

For research use only. We do not sell to patients.

eIF4A3-IN-2 Chemical Structure

CAS No. : 2095677-20-4

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 729	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 729	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 550	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 850	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 2650	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 3950	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of eIF4A3-IN-2:

(R)-eIF4A3-IN-2 Get quote

1 Publications Citing Use of MCE eIF4A3-IN-2

•Nucleic Acids Res. 2022 Nov 23;gkac1084. [Abstract]

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Purity & Documentation
References
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Description

eIF4A3-IN-2 is a highly selective and noncompetitive eukaryotic initiation factor 4A-3 (eIF4A3) inhibitor with an IC₅₀ of 110 nM^[1].

IC₅₀ & Target

eIF4

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	GI₅₀	3.06 μM Compound: 1a	Growth inhibition of human HCT116 cells after 3 days by CellTiter-Glo luminescence assay Growth inhibition of human HCT116 cells after 3 days by CellTiter-Glo luminescence assay	[PMID: 29057054]

In Vitro

eIF4A3-IN-2 (compound 2) binds to the allosteric region in eIF4A3 and inhibits in vitro ATPase, helicase, and cellular nonsense-mediated RNA decay (NMD) activities^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:	HEK293T cells (transfected with the NMD reporter) luciferase assay
Concentration:	0.3, 1, 3, or 10 μM
Incubation Time:	3 or 6 h
Result:	eIF4A3-IN-2 induces an approximately 3.2-fold increase in luciferase activity, indicating that NMD is inhibited by compound 2.

Molecular Weight

602.70

Formula

C₂₅H₁₉Br₂ClN₄O₂

CAS No.

2095677-20-4

Appearance

Solid

Color

White to light yellow

SMILES

O=C(C1=CC=C(Br)C=C1)N2CCN(C(C3=C4N(C=C(Br)C=C4)N=C3)=O)C[C@@H]2C5=CC=C(Cl)C=C5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (165.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6592 mL	8.2960 mL	16.5920 mL
5 mM	0.3318 mL	1.6592 mL	3.3184 mL
10 mM	0.1659 mL	0.8296 mL	1.6592 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 5% DMSO 40% PEG300 5% Tween-80 50% Saline
Solubility: 2.5 mg/mL (4.15 mM); Suspended solution; Need ultrasonic
Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.67 mg/mL (2.77 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.67 mg/mL (2.77 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.70%

Select Batch:

Data Sheet (279 KB) SDS (252 KB)

COA (258 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Iwatani-Yoshihara M, et al. Discovery and Characterization of a Eukaryotic Initiation Factor 4A-3-Selective Inhibitor That Suppresses Nonsense-Mediated mRNA Decay. ACS Chem Biol. 2017 Jul 21;12(7):1760-1768. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.6592 mL	8.2960 mL	16.5920 mL	41.4800 mL
	5 mM	0.3318 mL	1.6592 mL	3.3184 mL	8.2960 mL
	10 mM	0.1659 mL	0.8296 mL	1.6592 mL	4.1480 mL
	15 mM	0.1106 mL	0.5531 mL	1.1061 mL	2.7653 mL
	20 mM	0.0830 mL	0.4148 mL	0.8296 mL	2.0740 mL
	25 mM	0.0664 mL	0.3318 mL	0.6637 mL	1.6592 mL
	30 mM	0.0553 mL	0.2765 mL	0.5531 mL	1.3827 mL
	40 mM	0.0415 mL	0.2074 mL	0.4148 mL	1.0370 mL
	50 mM	0.0332 mL	0.1659 mL	0.3318 mL	0.8296 mL
	60 mM	0.0277 mL	0.1383 mL	0.2765 mL	0.6913 mL
	80 mM	0.0207 mL	0.1037 mL	0.2074 mL	0.5185 mL
	100 mM	0.0166 mL	0.0830 mL	0.1659 mL	0.4148 mL

eIF4A3-IN-2 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

eIF4A3-IN-22095677-20-4Eukaryotic Initiation Factor (eIF)AutophagyInhibitorinhibitorinhibit

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eIF4A3-IN-2

Customer Review

Other Forms of eIF4A3-IN-2:

eIF4A3-IN-2 Related Antibodies

1 Publications Citing Use of MCE eIF4A3-IN-2

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

eIF4A3-IN-2 Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

eIF4A3-IN-2 Related Classifications

Keywords:

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