Flavonol

Name: Flavonol
Brand: MedChemExpress
SKU: HY-107825
Rating: 4.5 (1 reviews)

Cat. No.: HY-107825 Purity: 99.92%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Flavonol is a cholinesterase (ChE) inhibitor, with an IC₅₀ value of 120 μM and a K_i value of 74 μM. Flavonol has antioxidant, free radical-scavenging, antibacterial properties, and immune modulation functions. Flavonol inhibits the PriA helicase of Staphylococcus aureus. Flavonol can suppress the production of NO in LPS-activated RAW 264.7 cells by inhibiting the expression of the iNOS enzyme. Flavonol shows protective and analgesic effects in mice through various neuronal pathways. Flavonol can be used in research related to tumors and atherosclerosis diseases.

For research use only. We do not sell to patients.

Flavonol Chemical Structure

CAS No. : 577-85-5

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 28	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 28	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	USD 25	In-stock	Estimated Time of Arrival: December 31
1 g	USD 40	In-stock	Estimated Time of Arrival: December 31
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10 g		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target

IC₅₀: 120 μM (ChE)^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
Caco-2	IC₅₀	42.1 μM Compound: 25	Cytotoxicity in human Caco2 cells after 72 hrs by MTT assay Cytotoxicity in human Caco2 cells after 72 hrs by MTT assay	[PMID: 28793973]
CWR22R	IC₅₀	14.9 μM Compound: Flavonol	Antiproliferative activity against human 22Rv1 cells incubated up to 144 hrs by Coulter particle count and size analyzer Antiproliferative activity against human 22Rv1 cells incubated up to 144 hrs by Coulter particle count and size analyzer	[PMID: 22789812]
H9	EC₅₀	13 μM Compound: 13	Antiviral activity against HIV1 3B infected in human H9 cells assessed as inhibition of viral replication after 3 days by p24 antigen capture assay Antiviral activity against HIV1 3B infected in human H9 cells assessed as inhibition of viral replication after 3 days by p24 antigen capture assay	[PMID: 8158164]
H9	IC₅₀	17 μM Compound: 13	Cytotoxicity against human H9 cells after 3 days Cytotoxicity against human H9 cells after 3 days	[PMID: 8158164]

In Vitro

Flavonol (10-100 μM, 20 h) can inhibit LPS activated RAW 264.7 cells from producing NO by suppressing iNOS enzyme expression, thereby contributing to its anti-inflammatory and immunomodulatory activities^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3].

Cell Line:	RAW 264.7 cell
Concentration:	10-100 μM
Incubation Time:	20 h
Result:	Inhibited the protein expression level of iNOS enzyme.

In Vivo

Flavonol (25-200 mg/kg; 30 min before the testing procedure; s.c.) has anti-injury and analgesic effects in mice through neuronal pathways such as the dopaminergic system and GABAergic pathway^[4].
Flavonol can reduce the atherosclerotic lesion area in apolipoprotein E deficient mice^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Inbred male Swiss albino mouse model induced by acetic acid induced abdominal contractions, formalin induced pain sensation, and hot tail soaking test^[4].
Dosage:	25, 50, 100, 200 mg/kg
Administration:	Subcutaneous injection (s.c.); 30 min before the testing procedure
Result:	Resulted in a significant reduction in the number of abdominal constrictions in the acetic acid test, a significant inhibition of the paw-licking/biting response time in both the phases of formalin nociception and also a significant increase in mean reaction time in the hot water tail immersion test.

Molecular Weight

238.24

Formula

C₁₅H₁₀O₃

CAS No.

577-85-5

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C(O)=C(C2=CC=CC=C2)OC3=CC=CC=C13

Structure Classification

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL (139.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.1974 mL	20.9872 mL	41.9745 mL
5 mM	0.8395 mL	4.1974 mL	8.3949 mL
10 mM	0.4197 mL	2.0987 mL	4.1974 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (10.49 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (10.49 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (10.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (300 KB) SDS (393 KB)

COA (252 KB) HNMR (119 KB) RP-HPLC (186 KB)

Handling Instructions (2659 KB)

References

[1]. Huang YH, et al. Inhibition of Staphylococcus aureus PriA Helicase by Flavonol Kaempferol. Protein J. 2015 Jun;34(3):169-72. [Content Brief]

[2]. Guo AJ, et al. Galangin, a flavonol derived from Rhizoma Alpiniae Officinarum, inhibits acetylcholinesterase activity in vitro. Chem Biol Interact. 2010 Sep 6;187(1-3):246-8. [Content Brief]

[3]. Kim HK, et al. Effects of naturally occurring flavonoids on nitric oxide production in the macrophage cell line RAW 264.7 and their structure-activity relationships. Biochem Pharmacol. 1999 Sep 1;58(5):759-65. [Content Brief]

[4]. Sayeli V, et al. Antinociceptive effect of flavonol and a few structurally related dimethoxy flavonols in mice. Inflammopharmacology. 2019 Dec;27(6):1155-1167. [Content Brief]

[5]. Phie J, et al. Flavonols reduce aortic atherosclerosis lesion area in apolipoprotein E deficient mice: A systematic review and meta-analysis. PLoS One. 2017 Jul 25;12(7):e0181832. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.1974 mL	20.9872 mL	41.9745 mL	104.9362 mL
	5 mM	0.8395 mL	4.1974 mL	8.3949 mL	20.9872 mL
	10 mM	0.4197 mL	2.0987 mL	4.1974 mL	10.4936 mL
	15 mM	0.2798 mL	1.3991 mL	2.7983 mL	6.9957 mL
	20 mM	0.2099 mL	1.0494 mL	2.0987 mL	5.2468 mL
	25 mM	0.1679 mL	0.8395 mL	1.6790 mL	4.1974 mL
	30 mM	0.1399 mL	0.6996 mL	1.3991 mL	3.4979 mL
	40 mM	0.1049 mL	0.5247 mL	1.0494 mL	2.6234 mL
	50 mM	0.0839 mL	0.4197 mL	0.8395 mL	2.0987 mL
	60 mM	0.0700 mL	0.3498 mL	0.6996 mL	1.7489 mL
	80 mM	0.0525 mL	0.2623 mL	0.5247 mL	1.3117 mL
	100 mM	0.0420 mL	0.2099 mL	0.4197 mL	1.0494 mL

Flavonol Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Flavonol577-85-5Endogenous MetaboliteBacterialCholinesterase (ChE)acetylcholinesteraseantioxidantanti-bacterialimmune regulationatherosclerosisInhibitorinhibitorinhibit

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Flavonol

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Complete Stock Solution Preparation Table

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