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FR054 is an inhibitor of the HBP enzyme PGM3, with a remarkable anti-breast cancer effect.

For research use only. We do not sell to patients.

FR054 Chemical Structure

FR054 Chemical Structure

CAS No. : 35954-65-5

Size Price Stock Quantity
Oil + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 In-stock
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Oil
5 mg USD 50 In-stock
10 mg USD 75 In-stock
50 mg USD 160 In-stock
100 mg USD 250 In-stock
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500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of FR054:

FR054 Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

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Description

FR054 is an inhibitor of the HBP enzyme PGM3, with a remarkable anti-breast cancer effect[1].

In Vitro

FR054 (0.5-1 mM, 24-48 h) induces an early proliferation arrest followed by a marked cell death increase in breast cancer cells and induces apoptosis. The effect of FR054 occurs through PGM3 inhibition instead of other off-target effects[1].
? FR054 (250?μM, 24 h) treatment efficiently affects both N- and O-glycosylation levels in MDA-MB-231 cells[1].
? FR054 induces endoplasmic reticulum (ER) stress and a ROS-dependent apoptotic cell death[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

FR054 Related Antibodies

Cell Viability Assay[1]

Cell Line: MDA-MB-231 cells.
Concentration: 0.5-1 mM.
Incubation Time: 48 h.
Result: Reduced viability and a significant increase of the apoptosis as compared to the control clone.
In Vivo

FR054 (1000?mg/kg, ip) suppresses cancer growth in MDA-MB-231 xenograft mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Mice were subcutaneously injected with MDA-MB-231 cells[1].
Dosage: 1000 mg/kg.
Administration: IP, single or fractionated dose (twice a day 500 mg/kg/dose).
Result: Appears to have an in vivo antitumor efficacy that is higher when administered twice a day compared to single administration.
Molecular Weight

329.30

Formula

C14H19NO8
CAS No.
Appearance

Oil

Color

Colorless to light yellow

SMILES

CC(O[C@@H]1[C@]2([H])[C@](OC(C)=N2)([H])O[C@@H]([C@H]1OC(C)=O)COC(C)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (303.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0367 mL 15.1837 mL 30.3674 mL
5 mM 0.6073 mL 3.0367 mL 6.0735 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.59 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (7.59 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  50% PEG300    50% Saline

    Solubility: 25 mg/mL (75.92 mM); Clear solution; Need ultrasonic

  • Protocol 2

    Add each solvent one by one:  15% Cremophor EL    85% Saline

    Solubility: 25 mg/mL (75.92 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥97.0%

COA (255 KB) HNMR (121 KB) MS (181 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0367 mL 15.1837 mL 30.3674 mL 75.9186 mL
5 mM 0.6073 mL 3.0367 mL 6.0735 mL 15.1837 mL
10 mM 0.3037 mL 1.5184 mL 3.0367 mL 7.5919 mL
15 mM 0.2024 mL 1.0122 mL 2.0245 mL 5.0612 mL
20 mM 0.1518 mL 0.7592 mL 1.5184 mL 3.7959 mL
25 mM 0.1215 mL 0.6073 mL 1.2147 mL 3.0367 mL
30 mM 0.1012 mL 0.5061 mL 1.0122 mL 2.5306 mL
40 mM 0.0759 mL 0.3796 mL 0.7592 mL 1.8980 mL
50 mM 0.0607 mL 0.3037 mL 0.6073 mL 1.5184 mL
60 mM 0.0506 mL 0.2531 mL 0.5061 mL 1.2653 mL
80 mM 0.0380 mL 0.1898 mL 0.3796 mL 0.9490 mL
100 mM 0.0304 mL 0.1518 mL 0.3037 mL 0.7592 mL
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FR054 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

FR05435954-65-5FR 054FR-054OthersTripleNegativeBreastCancerTNBCHBPPGM3Inhibitorinhibitorinhibit

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