2. PI3K/Akt/mTOR
  3. PI3K
  4. Gallein

Gallein  (Synonyms: Pyrogallol phthalein)

Cat. No.: HY-D0254 Purity: ≥98.0%
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Gallein is a Gβγ subunit signaling inhibitor that can interfere with the interaction between Gβγ subunit and PI3Kγ, regulate platelet function, and exhibit anti-tumor activity[1][3].

For research use only. We do not sell to patients.

Gallein Chemical Structure

Gallein Chemical Structure

CAS No. : 2103-64-2

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10 mM * 1 mL in DMSO
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10 mg USD 55 In-stock
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Based on 4 publication(s) in Google Scholar

Gallein Related Antibodies

Top Publications Citing Use of Products

View All PI3K Isoform Specific Products:

View All Isoforms
PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

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Description

Gallein is a Gβγ subunit signaling inhibitor that can interfere with the interaction between Gβγ subunit and PI3Kγ, regulate platelet function, and exhibit anti-tumor activity[1][3].
IC50 & Target

Gβγ subunit
In Vitro

Gallein (10  μM, 0-150 h) inhibits β-ionone induced cell invasion in LNCaP prostate cancer cells[1].
Gallein (30 μM, 1 h) increases osteoprotegerin (OPG) synthesis induced by fibroblast growth factor 2 (FGF-2) in osteoblasts[2].
Gallein (25-50 μM, 24 h) inhibits platelet aggregation, secretion, and diffusion functions[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Gallein Related Antibodies

Cell Invasion Assay[1].

Cell Line: LNCaP cell
Concentration: 10  μM
Incubation Time: 0-150 h
Result: Inhibited LNCaP cell invasion

Real Time qPCR[2].

Cell Line: Osteoblast-like MC3T3-E1 cell
Concentration: 30  μM
Incubation Time: 1 h
Result: Enhanced the FGF-2-induced OPG/Tnfrsf11b mRNA expression

Western Blot Analysis[3].

Cell Line: Platelet
Concentration: 25-50  μM
Incubation Time: 24 h
Result: Reduced phosphorylation of Akt
In Vivo

Gallein (5 mg/kg, 15 days, i.p.) inhibits tumor cell proliferation in male NSG mice[1].
Gallein (20 mg/kg, b.i.d, i.v.) regulates hemostasis and prevents arterial thrombosis in C57BL/6 mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Castrated male NSG mice[1].
Dosage: 5 mg/kg
Administration: Intraperitoneal injection (i.p.); 15 days
Result: Reduced the number of metastasised tissues
Animal Model: C57BL/6 mice[3].
Dosage: 20 mg/kg
Administration: Intravenous injection (i.v.); twice per day; 3 days
Result: Extended tail bleeding time
Molecular Weight

364.31

Formula

C20H12O7
CAS No.
Appearance

Solid

Color

Light brown to black

SMILES

O=C1OC2(C3=C(OC4=C2C=CC(O)=C4O)C(O)=C(O)C=C3)C5=C1C=CC=C5
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 33.33 mg/mL (91.49 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7449 mL 13.7246 mL 27.4492 mL
5 mM 0.5490 mL 2.7449 mL 5.4898 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.86 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.86 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7449 mL 13.7246 mL 27.4492 mL 68.6229 mL
5 mM 0.5490 mL 2.7449 mL 5.4898 mL 13.7246 mL
10 mM 0.2745 mL 1.3725 mL 2.7449 mL 6.8623 mL
15 mM 0.1830 mL 0.9150 mL 1.8299 mL 4.5749 mL
20 mM 0.1372 mL 0.6862 mL 1.3725 mL 3.4311 mL
25 mM 0.1098 mL 0.5490 mL 1.0980 mL 2.7449 mL
30 mM 0.0915 mL 0.4575 mL 0.9150 mL 2.2874 mL
40 mM 0.0686 mL 0.3431 mL 0.6862 mL 1.7156 mL
50 mM 0.0549 mL 0.2745 mL 0.5490 mL 1.3725 mL
60 mM 0.0457 mL 0.2287 mL 0.4575 mL 1.1437 mL
80 mM 0.0343 mL 0.1716 mL 0.3431 mL 0.8578 mL
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Gallein Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Gallein2103-64-2Pyrogallol phthaleinPI3KPhosphoinositide 3-kinaseplatelet functionthrombosisMC3T3-E1 cellInhibitorinhibitorinhibit

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