Smad3 - SMAD family member 3 Gene

Mus musculus

Also known as Madh3

Gene ID: 17127 | Gene type: protein coding

About Smad3

Summary

Enables several functions, including DNA binding activity; DNA-binding transcription activator activity, RNA polymerase II-specific; and promoter-specific chromatin binding activity. Involved in several processes, including JNK cascade; negative regulation of osteoblast proliferation; and positive regulation of chondrocyte differentiation. Acts upstream of or within several processes, including animal organ development; regulation of transcription by RNA polymerase II; and transmembrane receptor protein serine/threonine kinase signaling pathway. Located in cytoplasm; nucleus; and plasma membrane. Is expressed in several structures, including alimentary system; early conceptus; genitourinary system; limb; and nervous system. Used to study osteoarthritis. Human ortholog(s) of this gene implicated in Loeys-Dietz syndrome 3; Lynch syndrome; and pancreatic Cancer. Orthologous to human SMAD3 (Smad Family member 3). [provided by Alliance of Genome Resources, Apr 2022]

Smad3 Products(1)

mRNA	Protein	Name
NM_016769.4	NP_058049.3	mothers against decapentaplegic homolog 3

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	Reference	Source
enables DNA binding	IDA IDA: Inferred from direct assay	27336669	MGI
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IPI IPI: Inferred from physical interaction	24964035	MGI
enables DNA-binding transcription factor activity	IDA IDA: Inferred from direct assay	12943993	MGI
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IDA IDA: Inferred from direct assay	25065622	MGI
enables RNA polymerase II-specific DNA-binding transcription factor binding	IPI IPI: Inferred from physical interaction	11937490	MGI
enables chromatin DNA binding	IDA IDA: Inferred from direct assay	16224064	MGI
enables chromatin binding	IDA IDA: Inferred from direct assay	16619037	MGI
enables collagen binding	IPI IPI: Inferred from physical interaction	14559231	MGI
enables double-stranded DNA binding	IDA IDA: Inferred from direct assay	15282343	MGI
enables identical protein binding	IPI IPI: Inferred from physical interaction	21724602	MGI
enables promoter-specific chromatin binding	IDA IDA: Inferred from direct assay	18802055	MGI
enables protein binding	IPI IPI: Inferred from physical interaction	11711431	MGI

Biological Process GO Annotation	Evidence	Reference	Source
involved in JNK cascade	IMP IMP: Inferred from mutant phenotype	18802055	MGI
involved in MAPK cascade	IMP IMP: Inferred from mutant phenotype	18802055	MGI
acts upstream of or within SMAD protein signal transduction	IGI IGI: Inferred from genetic interaction	23390484	MGI
involved in T cell activation	IMP IMP: Inferred from mutant phenotype	10064594	MGI
acts upstream of or within apoptotic process	IMP IMP: Inferred from mutant phenotype	19679673	MGI
acts upstream of or within cell population proliferation	IGI IGI: Inferred from genetic interaction	19679673	MGI
involved in cellular response to virus	IDA IDA: Inferred from direct assay	18802055	MGI
acts upstream of or within developmental growth	IGI IGI: Inferred from genetic interaction	15183723	MGI
acts upstream of or within embryonic cranial skeleton morphogenesis	IGI IGI: Inferred from genetic interaction	15183723	MGI
acts upstream of or within embryonic foregut morphogenesis	IGI IGI: Inferred from genetic interaction	15183723	MGI
acts upstream of or within embryonic pattern specification	IGI IGI: Inferred from genetic interaction	15183723	MGI
acts upstream of or within endoderm development	IGI IGI: Inferred from genetic interaction	15183723	MGI
acts upstream of or within gastrulation	IGI IGI: Inferred from genetic interaction	15183723	MGI
acts upstream of or within heart looping	IGI IGI: Inferred from genetic interaction	15183723	MGI
acts upstream of or within immune system development	IGI IGI: Inferred from genetic interaction	15183723	MGI
acts upstream of or within in utero embryonic development	IGI IGI: Inferred from genetic interaction	15183723	MGI
acts upstream of or within lens fiber cell differentiation	IMP IMP: Inferred from mutant phenotype	17215516	MGI
acts upstream of or within liver development	IGI IGI: Inferred from genetic interaction	15183723	MGI
acts upstream of or within mesoderm formation	IMP IMP: Inferred from mutant phenotype	15084457	MGI
involved in negative regulation of inflammatory response	IMP IMP: Inferred from mutant phenotype	10559937	MGI
acts upstream of or within negative regulation of osteoblast differentiation	IGI IGI: Inferred from genetic interaction	15150273	MGI
involved in negative regulation of osteoblast proliferation	IMP IMP: Inferred from mutant phenotype	21035443	MGI
acts upstream of or within negative regulation of transcription by RNA polymerase II	IDA IDA: Inferred from direct assay	9702197	MGI
involved in negative regulation of wound healing	IMP IMP: Inferred from mutant phenotype	10559937	MGI
acts upstream of or within osteoblast development	IGI IGI: Inferred from genetic interaction	15150273	MGI
involved in osteoblast differentiation	IMP IMP: Inferred from mutant phenotype	11585338	MGI
acts upstream of or within paraxial mesoderm morphogenesis	IMP IMP: Inferred from mutant phenotype	15084457	MGI
acts upstream of or within pericardium development	IGI IGI: Inferred from genetic interaction	15183723	MGI
acts upstream of or within positive regulation of DNA-templated transcription	IDA IDA: Inferred from direct assay	15282343	MGI
involved in positive regulation of chondrocyte differentiation	IMP IMP: Inferred from mutant phenotype	21035443	MGI
acts upstream of or within positive regulation of transcription by RNA polymerase II	IDA IDA: Inferred from direct assay	12943993	MGI
involved in positive regulation of transcription by RNA polymerase II	IDA IDA: Inferred from direct assay	25065622	MGI
acts upstream of or within positive regulation of transcription by RNA polymerase II	IGI IGI: Inferred from genetic interaction	15150273	MGI
involved in positive regulation of transcription by RNA polymerase II	IMP IMP: Inferred from mutant phenotype	18802055	MGI
acts upstream of or within regulation of binding	IDA IDA: Inferred from direct assay	15282343	MGI
acts upstream of or within regulation of epithelial cell proliferation	IMP IMP: Inferred from mutant phenotype	12845704	MGI
involved in regulation of immune response	IMP IMP: Inferred from mutant phenotype	10064594	MGI
acts upstream of or within regulation of striated muscle tissue development	IDA IDA: Inferred from direct assay	11711431	MGI
acts upstream of or within regulation of transforming growth factor beta receptor signaling pathway	IDA IDA: Inferred from direct assay	11711431	MGI
acts upstream of or within response to transforming growth factor beta	IGI IGI: Inferred from genetic interaction	19679673	MGI
acts upstream of or within skeletal system development	IGI IGI: Inferred from genetic interaction	15183723	MGI
acts upstream of or within somitogenesis	IMP IMP: Inferred from mutant phenotype	15183723	MGI
acts upstream of or within thyroid gland development	IGI IGI: Inferred from genetic interaction	15183723	MGI
acts upstream of or within transforming growth factor beta receptor signaling pathway	IDA IDA: Inferred from direct assay	15239668	MGI
acts upstream of or within transforming growth factor beta receptor signaling pathway	IMP IMP: Inferred from mutant phenotype	15239668	MGI
involved in ureteric bud development	IEP IEP: Inferred from expression pattern	14656760	MGI

Cellular Component GO Annotation	Evidence	Reference	Source
located in cytoplasm	IDA IDA: Inferred from direct assay	11160896	MGI
located in nucleus	IDA IDA: Inferred from direct assay	11160896	MGI
located in plasma membrane	IDA IDA: Inferred from direct assay	12543979	MGI

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

Protein Preferred Names

Protein Names

mothers against decapentaplegic homolog 3

MAD homolog 3	Smad 3
mMad3	mad3
mothers against DPP homolog 3

Smad3 Protein-protein interaction Information

Type Cross Intra	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	Reference
Intra	Smad3	Q8BUN5	Pou5f1	Mus musculus	P20263	Anti Bait CoIP	22036565
Intra	Smad3	Q8BUN5	Pou5f1	Mus musculus	P20263	CH-IP	22036565
Intra	Smad3	Q8BUN5	Pou5f1	Mus musculus	P20263	Crosslink	22036565
Intra	Smad3	Q8BUN5	Axin1	Mus musculus	O35625	Anti Tag CoIP	16601693
Intra	Smad3	Q8BUN5	Smad4	Mus musculus	P97471	Anti Bait CoIP	18268325
Intra	Smad3	Q8BUN5	Smad4	Mus musculus	P97471	Anti Bait CoIP	22045334
Intra	Smad3	Q8BUN5	Smad4	Mus musculus	P97471	Anti Bait CoIP	22036565
Intra	Smad3	Q8BUN5	Smad4	Mus musculus	P97471	PLA	22442258

Cross: Cross-species interaction Intra: Intraspecies interaction

Recombinant Smad3 Proteins

Cat. No.	Product Name	Accession	Purity
HY-P73636	SMAD3 Protein, Human/Mouse/Rat (sf9, His-GST)	Q8BUN5 (M1-S425)	≥95%

Related Agents

Pre-clinical Phase

Bioactive Molecules (18)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-13013	SIS3	Inhibitor	98.71%	Unkown
HY-12953	R-268712	Inhibitor	99.88%	Unkown
HY-W012977	3,3-Dimethyl-1-butanol	Inhibitor	99.56%	Unkown
HY-47888	PLN-1474	Inhibitor	99.30%	Unkown
HY-18758	IN-1130	Inhibitor	99.93%	Unkown
HY-N2033	Chebulinic acid	Inhibitor	99.68%	Unkown
HY-100444	SIS3 free base	Inhibitor	98.11%	Unkown
HY-144043	ALK5-IN-8	Inhibitor	98.06%	Unkown
HY-147025	(S,R,S)-AHPC-C2-amide-benzofuranylmethyl-pyridine		99.06%	Unkown
HY-114495	Caerulomycin A	Inhibitor	≥98.0%	Unkown
HY-128439	BT173	Inhibitor	98.71%	Unkown
HY-114683	KS370G		99.71%	Unkown
HY-146434	TGFβ-IN-2	Inhibitor	99.37%	Unkown
HY-163536	TGF-β1/Smad3-IN-1	Inhibitor	98.05%	Unkown
HY-W803134	(E/Z)-SIS3 free base	Inhibitor	99.48%	Unkown
HY-P10363	Tiger17	Activator	/	Unkown
HY-RS13394	SMAD3 Human Pre-designed siRNA Set A	Pre-designed Sets	/	Unkown
HY-W108953	Myristoyl tetrapeptide-12	Activator	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Homo sapiens	Smad3	NCBI	NCBI:4088
Rattus norvegicus	Smad3	NCBI	NCBI:25631

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