RPA1 - replication protein A1 Gene

Homo sapiens

Also known as HSSB; RF-A; RP-A; REPA1; RPA70; MST075; PFBMFT6

Gene ID: 6117 | Gene type: protein coding

About RPA1

Cytogenetic location: 17p13.3 Genomic coordinates (GRCh38): 17:1,830,005-1,900,082 (from NCBI)

This gene has 7 transcripts (splice variants), 268 orthologues and is associated with 1 phenotype. Ubiquitous expression in lymph node (RPKM 21.6), ovary (RPKM 19.7) and 25 other tissues.

Summary

This gene encodes the largest subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The nucleoprotein complex protects the single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. This subunit contains four oligonucleotide/oligosaccharide-binding (OB) domains, though the majority of ssDNA binding occurs in two of these domains. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which ssDNA binding domains are utilized. The different binding modes differ in the length of DNA bound and in the proteins with which it interacts, thereby playing a role in regulating different genomic maintenance pathways. [provided by RefSeq, Sep 2017]

RPA1 Products(3)

mRNA	Protein	Name
NM_001355120.2	NP_001342049.1	replication protein A 70 kDa DNA-binding subunit isoform 2
NM_001355121.2	NP_001342050.1	replication protein A 70 kDa DNA-binding subunit isoform 3
NM_002945.5	NP_002936.1	replication protein A 70 kDa DNA-binding subunit isoform 1

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	Reference	Source
enables G-rich strand telomeric DNA binding	IDA IDA: Inferred from direct assay	16973897	GOA
enables G-rich strand telomeric DNA binding	IMP IMP: Inferred from mutant phenotype	26041456	GOA
enables chromatin-protein adaptor activity	IDA IDA: Inferred from direct assay	35597237	GOA
enables damaged DNA binding	IDA IDA: Inferred from direct assay	7700386	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	1311258	GOA
enables single-stranded DNA binding	IDA IDA: Inferred from direct assay	8990123	GOA

Biological Process GO Annotation	Evidence	Reference	Source
involved in DNA damage response	IMP IMP: Inferred from mutant phenotype	27248496	GOA
involved in DNA repair	IDA IDA: Inferred from direct assay	20545304	GOA
involved in DNA repair	IMP IMP: Inferred from mutant phenotype	18469000	GOA
involved in DNA replication	IMP IMP: Inferred from mutant phenotype	9430682	GOA
involved in base-excision repair	IDA IDA: Inferred from direct assay	9765279	GOA
involved in double-strand break repair via homologous recombination	IMP IMP: Inferred from mutant phenotype	17765923	GOA
involved in mismatch repair	IMP IMP: Inferred from mutant phenotype	9430682	GOA
involved in nucleotide-excision repair	IMP IMP: Inferred from mutant phenotype	9430682	GOA
involved in protein localization to chromosome	IDA IDA: Inferred from direct assay	27723717	GOA
involved in protein localization to site of double-strand break	IDA IDA: Inferred from direct assay	35597237	GOA
involved in telomere maintenance	IMP IMP: Inferred from mutant phenotype	17959650	GOA

Cellular Component GO Annotation	Evidence	Reference	Source
part of DNA replication factor A complex	IDA IDA: Inferred from direct assay	11927569	GOA
part of DNA replication factor A complex	IPI IPI: Inferred from physical interaction	7760808	GOA
colocalizes with PML body	IDA IDA: Inferred from direct assay	17959650	GOA
located in PML body	IDA IDA: Inferred from direct assay	16135809	GOA
located in nucleus	IDA IDA: Inferred from direct assay	17959650	GOA
located in site of DNA damage	IMP IMP: Inferred from mutant phenotype	27248496	GOA
is active in site of double-strand break	IDA IDA: Inferred from direct assay	35597237	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

RPA1 Protein Structure

Rep-A_N

tRNA_anti-codon

Rep_fac-A_C

0
100
200
300
400
500
616 a.a.

Protein Preferred Names

Protein Names

replication protein A 70 kDa DNA-binding subunit

MSTP075

RPA1 Protein-protein interaction Information

Type Cross Intra	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	Reference
Intra	RPA1	P27694	PRIMPOL	Homo sapiens	Q96LW4	Anti Tag CoIP	24126761
Intra	RPA1	P27694	PRIMPOL	Homo sapiens	Q96LW4	TAP	24126761
Intra	RPA1	P27694	PRIMPOL	Homo sapiens	Q96LW4	Pull Down	24126761
Intra	RPA1	P27694	MTUS2	Homo sapiens	Q5JR59-3	Y2H Prey Pooling	32296183
Intra	RPA1	P27694	MTUS2	Homo sapiens	Q5JR59-3	Validated Y2H	32296183
Intra	RPA1	P27694	MTUS2	Homo sapiens	Q5JR59-3	Y2H Array	32296183
Intra	RPA1	P27694	RPA4	Homo sapiens	Q13156	Anti Bait CoIP	7760808
Intra	RPA1	P27694	RPA4	Homo sapiens	Q13156	Y2H	7760808
Intra	RPA1	P27694	HSPA6	Homo sapiens	P17066	Y2H Prey Pooling	32296183
Intra	RPA1	P27694	HSPA6	Homo sapiens	P17066	Y2H Array	32296183
Intra	RPA1	P27694	WRN	Homo sapiens	Q14191	Pull Down	15965237
Intra	RPA1	P27694	WRN	Homo sapiens	Q14191	TAP	24126761
Intra	RPA1	P27694	WRN	Homo sapiens	Q14191	ELISA	15965237
Intra	RPA1	P27694	WRN	Homo sapiens	Q14191	ELISA	15735006
Cross	RPA1	P27694	lt_sv40	Simian virus 40	P03070	CoIP	17666013
Cross	RPA1	P27694	lt_sv40	Simian virus 40	P03070	Experimental Interac	8381428
Intra	RPA1	P27694	BLM	Homo sapiens	P54132	TAP	24126761
Intra	RPA1	P27694	BLM	Homo sapiens	P54132	ELISA	15965237
Intra	RPA1	P27694	RPA2	Homo sapiens	P15927	TAP	24126761
Intra	RPA1	P27694	RPA2	Homo sapiens	P15927	TAP	24981860
Intra	RPA1	P27694	RPA2	Homo sapiens	P15927	Anti Tag CoIP	26496610
Intra	RPA1	P27694	RPA2	Homo sapiens	P15927	Y2H Array	32296183
Intra	RPA1	P27694	RPA2	Homo sapiens	P15927	Pull Down	20811460
Intra	RPA1	P27694	RPA2	Homo sapiens	P15927	X-Ray Diffraction	11927569
Intra	RPA1	P27694	RPA2	Homo sapiens	P15927	Y2H Prey Pooling	32296183
Intra	RPA1	P27694	RPA2	Homo sapiens	P15927	GMS	12527904
Intra	RPA1	P27694	RPA2	Homo sapiens	P15927	Anti Bait CoIP	7760808
Intra	RPA1	P27694	RPA2	Homo sapiens	P15927	Chromatography	2159011
Intra	RPA1	P27694	RPA3	Homo sapiens	P35244	Anti Bait CoIP	7760808
Intra	RPA1	P27694	RPA3	Homo sapiens	P35244	TAP	24981860
Intra	RPA1	P27694	RPA3	Homo sapiens	P35244	Y2H	19338310
Intra	RPA1	P27694	RPA3	Homo sapiens	P35244	TAP	24126761
Intra	RPA1	P27694	RPA3	Homo sapiens	P35244	Anti Tag CoIP	26496610
Intra	RPA1	P27694	RPA3	Homo sapiens	P35244	X-Ray Diffraction	11927569
Intra	RPA1	P27694	RPA3	Homo sapiens	P35244	Chromatography	2159011
Intra	RPA1	P27694	RPA3	Homo sapiens	P35244	GMS	12527904
Intra	RPA1	P27694	RPA3	Homo sapiens	P35244	Pull Down	20811460
Intra	RPA1	P27694	MTUS2	Homo sapiens	Q5JR59	Y2H Array	25416956
Intra	RPA1	P27694	MTUS2	Homo sapiens	Q5JR59	Y2H Prey Pooling	25416956
Intra	RPA1	P27694	POLA1	Homo sapiens	P09884	CoIP	17666013

Cross: Cross-species interaction Intra: Intraspecies interaction

Related Diseases

Diseases

Alias

Pulmonary Fibrosis And/Or Bone Marrow Failure, Telomere-Related, 6

PFBMFT6

Pulmonary Fibrosis, And/Or Bone Marrow Failure, Telomere-Related, 6

Ataxia-Telangiectasia

Ataxia Telangiectasia	Louis-Bar Syndrome
AT	At1
Ataxia-Telangiectasia Syndrome	Ataxia - Telangiectasia Variant
Boder-Sedgwick Syndrome	Louis Bar Syndrome
Cerebello-Oculocutaneous Telangiectasia	Immunodeficiency With Ataxia Telangiectasia
A-T	Ataxia Telangiectasia Syndrome
Atm	Telangiectasia, Cerebello-Oculocutaneous
Ataxia-Telangiectasia Variant

Werner Syndrome

Werner'S Syndrome	WRN
Adult Progeria	Ws
Adult Premature Ageing Syndrome	Adult Premature Aging Syndrome
Werners Syndrome

Xeroderma Pigmentosum, Variant Type

Xeroderma Pigmentosum	XPV
Xeroderma Pigmentosum Variant Type	Xeroderma Pigmentosum With Normal Dna Repair Rates
Photosensitivity With Defective Dna Synthesis	Xp
De Sanctis-Cacchione Syndrome	Desanctis-Cacchione Syndrome
Xeroderma Pigmentosa	Xerodermic Idiocy
Xeroderma Pigmentosum Variant	Xp - [Xeroderma Pigmentosum]
Atrophoderma Pigmentosum

Microphthalmia, Syndromic 10

MCOPS10	Moba
Microphthalmia And Brain Atrophy	Syndromic Microphthalmia 10
Microphthalmia-Brain Atrophy Syndrome	Microphthalmia Syndromic 10
Moba Syndrome	Syndromic Microphthalmia Type 10

Bloom Syndrome

BLM	Bs
Bls	Bloom-Torre-Machacek Syndrome
Microcephaly, Growth Restriction, And Increased Sister Chromatid Exchange 1	Mgrisce1
Congenital Telangiectatic Erythema	Congenital Telangiectatic Erythema Syndrome
Growth Deficiency, Sun-Sensitive, Telangiectatic, Hypo And Hyperpigmented Skin, Predisposition To Malignancy And Chromosomal Instability	Bloom'S Syndrome
Bsyn

Trichothiodystrophy

Ttd	Amish Brittle Hair Syndrome
Bids Syndrome	Brittle Hair-Intellectual Impairment-Decreased Fertility-Short Stature Syndrome
Ibids	Pibids
Trichothiodystrophy Syndromes

Alzheimer Disease 13

AD13	Alzheimer Disease-13
Alzheimer'S Disease 13	Alzheimer'S Disease 13, Late Onset

Nijmegen Breakage Syndrome

Berlin Breakage Syndrome	NBS
Microcephaly, Normal Intelligence And Immunodeficiency	Ataxia-Telangiectasia Variant
Ataxia-Telangiectasia Variant V1	Seemanova Syndrome Ii
Immunodeficiency-Microcephaly-Chromosomal Instability Syndrome	Seemanova Syndrome Type 2
At-V1	Microcephaly With Normal Intelligence, Immunodeficiency, And Lymphoreticular Malignancies
Nonsyndromal Microcephaly, Autosomal Recessive, With Normal Intelligence	Immunodeficiency, Microcephaly, And Chromosomal Instability
Microcephaly-Immunodeficiency-Lymphoreticuloma Syndrome	Microcephaly Immunodeficiency Lymphoreticuloma
Microcephaly With Normal Intelligence Immunodeficiency And Lymphoreticular Malignancies	Nonsyndromal Microcephaly Autosomal Recessive With Normal Intelligence
Seemanova Syndrome 2	Ataxia-Telangiectasia Variant 1
Seemanova Syndrome	At V1
Ataxia-Telangiectasia, Variant 1	Microcephaly-Immunodeficiency-Lymphoid Malignancy Syndrome
V-At	Ataxia Telangiectasia Variant V1

Haverhill Fever

Streptobacillosis	Streptobacillary Rat-Bite Fever
Streptobacillary Fever	Rat-Bite Fever Due To Streptobacillus Moniliformis
Erythema Arthriticum Epidemicum	Epidemic Arthritic Erythema

Xeroderma Pigmentosum, Complementation Group A

Xeroderma Pigmentosum Group A	Xp1
Xeroderma Pigmentosum, Group A	XPA
Xeroderma Pigmentosum I	Xeroderma Pigmentosum Complementation Group A
Xp Group A	Xp, Group A
Xeroderma Pigmentosum 1	Xeroderma Pigmentosum, Type 1
XP-A

Seckel Syndrome

Microcephalic Primordial Dwarfism	Bird-Headed Dwarfism
Harper'S Syndrome	Virchow-Seckel Dwarfism
Nanocephalic Dwarfism	Sckl
Seckel-Type Dwarfism

Fanconi Anemia, Complementation Group A

Fanconi Anemia	Fanconi Pancytopenia
Fanconi Anemia Complementation Group A	FANCA
Fa	Fanconi Panmyelopathy
Fanconi'S Anemia	Fanconi Anaemia
Fanconi'S Anaemia	Fanconi Hypoplastic Anemia
Estren-Dameshek Variant Of Fanconi Anemia	Estren-Dameshek Variant Of Fanconi Pancytopenia
Fanconi Anemia Estren-Dameshek Variant	Fanconis Anemia

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase

Bioactive Molecules (2)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-155655	JC-229	Inhibitor	97.07%	Unkown
HY-RS12142	RPA1 Human Pre-designed siRNA Set A	Pre-designed Sets	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Rattus norvegicus	RPA1	RGD	RGD:1307376
Felis catus	RPA1	VGNC	VGNC:64726
Canis familiaris	RPA1	VGNC	VGNC:45702
Bos taurus	RPA1	VGNC	VGNC:34094
Mus musculus	RPA1	MGD	MGI:1915525
Others	RPA1	NCBI

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