RPA2 - replication protein A2 Gene

Homo sapiens

Also known as REPA2; RPA32; RP-A p32; RP-A p34

Gene ID: 6118 | Gene type: protein coding

About RPA2

Cytogenetic location: 1p35.3 Genomic coordinates (GRCh38): 1:27,891,524-27,914,797 (from NCBI)

This gene has 5 transcripts (splice variants), 244 orthologues and 2 paralogues. Ubiquitous expression in testis (RPKM 29.0), lymph node (RPKM 26.6) and 25 other tissues.

Summary

This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways. [provided by RefSeq, Sep 2017]

RPA2 Products(5)

mRNA	Protein	Name
NM_001286076.2	NP_001273005.1	replication protein A 32 kDa subunit isoform 2
NM_001297558.1	NP_001284487.1	replication protein A 32 kDa subunit isoform 3
NM_001355128.2	NP_001342057.1	replication protein A 32 kDa subunit isoform 2
NM_001355129.2	NP_001342058.1	replication protein A 32 kDa subunit isoform 4
NM_002946.5	NP_002937.1	replication protein A 32 kDa subunit isoform 1

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	Reference	Source
enables G-rich strand telomeric DNA binding	IDA IDA: Inferred from direct assay	24747047	GOA
enables damaged DNA binding	IDA IDA: Inferred from direct assay	7700386	GOA
enables enzyme binding	IPI IPI: Inferred from physical interaction	19793862	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	2159011	GOA
enables protein phosphatase binding	IPI IPI: Inferred from physical interaction	20154705	GOA
enables single-stranded DNA binding	IDA IDA: Inferred from direct assay	19010961	GOA
enables ubiquitin protein ligase binding	IPI IPI: Inferred from physical interaction	21504906	GOA

Biological Process GO Annotation	Evidence	Reference	Source
involved in DNA replication	IDA IDA: Inferred from direct assay	2406247	GOA
involved in DNA replication	IMP IMP: Inferred from mutant phenotype	9430682	GOA
involved in base-excision repair	IDA IDA: Inferred from direct assay	9765279	GOA
involved in double-strand break repair via homologous recombination	IMP IMP: Inferred from mutant phenotype	17765923	GOA
involved in mismatch repair	IMP IMP: Inferred from mutant phenotype	9430682	GOA
involved in mitotic G1 DNA damage checkpoint signaling	IMP IMP: Inferred from mutant phenotype	21731742	GOA
involved in nucleotide-excision repair	IMP IMP: Inferred from mutant phenotype	9430682	GOA
involved in protein localization to chromosome	IDA IDA: Inferred from direct assay	27723717	GOA
involved in regulation of DNA damage checkpoint	IMP IMP: Inferred from mutant phenotype	21504906	GOA
involved in regulation of double-strand break repair via homologous recombination	IMP IMP: Inferred from mutant phenotype	20154705	GOA
involved in telomere maintenance	IMP IMP: Inferred from mutant phenotype	17959650	GOA

Cellular Component GO Annotation	Evidence	Reference	Source
part of DNA replication factor A complex	IDA IDA: Inferred from direct assay	2406247	GOA
part of DNA replication factor A complex	IPI IPI: Inferred from physical interaction	11927569	GOA
colocalizes with PML body	IDA IDA: Inferred from direct assay	17959650	GOA
located in PML body	IDA IDA: Inferred from direct assay	12814551	GOA
located in chromatin	IDA IDA: Inferred from direct assay	10336450	GOA
colocalizes with chromosome, telomeric region	IDA IDA: Inferred from direct assay	17959650	GOA
located in nucleus	IDA IDA: Inferred from direct assay	10982866	GOA
colocalizes with site of double-strand break	IDA IDA: Inferred from direct assay	21504906	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

RPA2 Protein Structure

tRNA_anti-codon

RPA_C

0
100
200
270 a.a.

Protein Preferred Names

Protein Names

replication protein A 32 kDa subunit

RF-A protein 2

RPA2 Protein-protein interaction Information

Type Cross Intra	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	Reference
Intra	RPA2	P15927	UNG	Homo sapiens	P13051	Y2H	10982866
Intra	RPA2	P15927	UNG	Homo sapiens	P13051	ITC	24910198
Intra	RPA2	P15927	RFWD3	Homo sapiens	Q6PCD5	TAP	24126761
Intra	RPA2	P15927	TIPIN	Homo sapiens	Q9BVW5	TAP	24126761
Intra	RPA2	P15927	TIPIN	Homo sapiens	Q9BVW5	ITC	24910198
Intra	RPA2	P15927	XPA	Homo sapiens	P23025	Y2H	10982866
Intra	RPA2	P15927	XPA	Homo sapiens	P23025	Validated Y2H	32296183
Intra	RPA2	P15927	GAPDH	Homo sapiens	P04406	Y2H Pooling	16169070
Intra	RPA2	P15927	CDC45	Homo sapiens	O75419	Pull Down	23910567
Intra	RPA2	P15927	CCNC	Homo sapiens	P24863	Validated Y2H	32296183
Intra	RPA2	P15927	SMARCAL1	Homo sapiens	Q9NZC9	X-Ray Diffraction	24910198
Intra	RPA2	P15927	SMARCAL1	Homo sapiens	Q9NZC9	GMS	24910198
Intra	RPA2	P15927	SMARCAL1	Homo sapiens	Q9NZC9	TAP	24126761
Intra	RPA2	P15927	SMARCAL1	Homo sapiens	Q9NZC9	ITC	24910198
Intra	RPA2	P15927	RPA1	Homo sapiens	P27694	Y2H	15965237
Intra	RPA2	P15927	RPA1	Homo sapiens	P27694	TAP	24126761
Intra	RPA2	P15927	RPA1	Homo sapiens	P27694	Anti Tag CoIP	33961781
Intra	RPA2	P15927	RPA1	Homo sapiens	P27694	Anti Tag CoIP	34591612
Intra	RPA2	P15927	RPA1	Homo sapiens	P27694	Validated Y2H	32296183
Intra	RPA2	P15927	RPA1	Homo sapiens	P27694	Anti Bait CoIP	24126761
Intra	RPA2	P15927	RPA3	Homo sapiens	P35244	Anti Tag CoIP	34591612
Intra	RPA2	P15927	RPA3	Homo sapiens	P35244	Y2H	19338310
Intra	RPA2	P15927	RPA3	Homo sapiens	P35244	TAP	24981860
Intra	RPA2	P15927	RPA3	Homo sapiens	P35244	TAP	24126761
Intra	RPA2	P15927	RPA3	Homo sapiens	P35244	Anti Tag CoIP	33961781
Intra	RPA2	P15927	RPA3	Homo sapiens	P35244	Y2H	10982866
Intra	RPA2	P15927	EIF4EBP3	Homo sapiens	O60516	Anti Tag CoIP	22684010
Intra	RPA2	P15927	SERTAD3	Homo sapiens	Q9UJW9	Y2H	10982866
Intra	RPA2	P15927	SERTAD3	Homo sapiens	Q9UJW9	Pull Down	10982866

Cross: Cross-species interaction Intra: Intraspecies interaction

RPA2 Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P80975	RPA32 Antibody (YA679)	WB, IHC-P, ICC/IF, IP	Human, Mouse, Rat
HY-P82975	Phospho-RPA2 (Thr21) Antibody (YA2720)	WB, IP	Human, Mouse, Rat

Related Diseases

Diseases

Alias

Ataxia-Telangiectasia

Ataxia Telangiectasia	Louis-Bar Syndrome
AT	At1
Ataxia-Telangiectasia Syndrome	Ataxia - Telangiectasia Variant
Boder-Sedgwick Syndrome	Louis Bar Syndrome
Cerebello-Oculocutaneous Telangiectasia	Immunodeficiency With Ataxia Telangiectasia
A-T	Ataxia Telangiectasia Syndrome
Atm	Telangiectasia, Cerebello-Oculocutaneous
Ataxia-Telangiectasia Variant

Xeroderma Pigmentosum, Variant Type

Xeroderma Pigmentosum	XPV
Xeroderma Pigmentosum Variant Type	Xeroderma Pigmentosum With Normal Dna Repair Rates
Photosensitivity With Defective Dna Synthesis	Xp
De Sanctis-Cacchione Syndrome	Desanctis-Cacchione Syndrome
Xeroderma Pigmentosa	Xerodermic Idiocy
Xeroderma Pigmentosum Variant	Xp - [Xeroderma Pigmentosum]
Atrophoderma Pigmentosum

Bloom Syndrome

BLM	Bs
Bls	Bloom-Torre-Machacek Syndrome
Microcephaly, Growth Restriction, And Increased Sister Chromatid Exchange 1	Mgrisce1
Congenital Telangiectatic Erythema	Congenital Telangiectatic Erythema Syndrome
Growth Deficiency, Sun-Sensitive, Telangiectatic, Hypo And Hyperpigmented Skin, Predisposition To Malignancy And Chromosomal Instability	Bloom'S Syndrome
Bsyn

Xeroderma Pigmentosum, Complementation Group A

Xeroderma Pigmentosum Group A	Xp1
Xeroderma Pigmentosum, Group A	XPA
Xeroderma Pigmentosum I	Xeroderma Pigmentosum Complementation Group A
Xp Group A	Xp, Group A
Xeroderma Pigmentosum 1	Xeroderma Pigmentosum, Type 1
XP-A

Xeroderma Pigmentosum, Complementation Group C

Xeroderma Pigmentosum, Group C	XPC
Xpcc	Xeroderma Pigmentosum Iii
Xp3	Xeroderma Pigmentosum Group C
Xp Group C	Xp, Group C
Xeroderma Pigmentosum, Type 3	Xeroderma Pigmentosum Complementation Group C
XP-C

Seckel Syndrome

Microcephalic Primordial Dwarfism	Bird-Headed Dwarfism
Harper'S Syndrome	Virchow-Seckel Dwarfism
Nanocephalic Dwarfism	Sckl
Seckel-Type Dwarfism

Fanconi Anemia, Complementation Group A

Fanconi Anemia	Fanconi Pancytopenia
Fanconi Anemia Complementation Group A	FANCA
Fa	Fanconi Panmyelopathy
Fanconi'S Anemia	Fanconi Anaemia
Fanconi'S Anaemia	Fanconi Hypoplastic Anemia
Estren-Dameshek Variant Of Fanconi Anemia	Estren-Dameshek Variant Of Fanconi Pancytopenia
Fanconi Anemia Estren-Dameshek Variant	Fanconis Anemia

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase

Bioactive Molecules (1)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-RS12143	RPA2 Human Pre-designed siRNA Set A	Pre-designed Sets	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Mus musculus	RPA2	MGD	MGI:1339939
Bos taurus	RPA2	VGNC	VGNC:34095
Felis catus	RPA2	VGNC	VGNC:80345
Macaca mulatta	RPA2	VGNC	VGNC:100064
Rattus norvegicus	RPA2	RGD	RGD:619714
Canis familiaris	RPA2	VGNC	VGNC:45703
Others	RPA2	NCBI

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