Hex

Name: Hex
Brand: MedChemExpress
SKU: HY-131904A
Rating: 4.5 (1 reviews)

Cat. No.: HY-131904A Purity: 99.96%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Hex is an enolase inhibitor, with K_i values of 74.4 nM and 269.4 nM for ENO2 and ENO1, respectively.

For research use only. We do not sell to patients.

Hex Chemical Structure

CAS No. : 2004714-32-1

Size	Price	Stock	Quantity
Solid or liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 715	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 715	In-stock	Estimated Time of Arrival: December 31
Solid or liquid
5 mg	USD 650	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 900	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1665	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Description

Hex is an enolase inhibitor, with K_i values of 74.4 nM and 269.4 nM for ENO2 and ENO1, respectively^[1].

IC₅₀ & Target

Ki: 74.4 nM (ENO2), 269.4 nM (ENO1)^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
D-423MG	IC₅₀	> 300000 nM Compound: 5; HEX	Cytotoxicity against human D-423MG cells expressing ENO1 assessed as cell growth inhibition incubated for 6 days by cystal violet staining based analysis Cytotoxicity against human D-423MG cells expressing ENO1 assessed as cell growth inhibition incubated for 6 days by cystal violet staining based analysis	[PMID: 36251833]
D-423MG	IC₅₀	1300 nM Compound: 5; HEX	Cytotoxicity against human D-423MG cells assessed as cell growth inhibition incubated for 6 days by cystal violet staining based analysis Cytotoxicity against human D-423MG cells assessed as cell growth inhibition incubated for 6 days by cystal violet staining based analysis	[PMID: 36251833]
D-423MG	IC₅₀	7574 nM Compound: HEX	Cytotoxicity against human D423 cells with ENO1 deficient assessed as reduction in cell viability incubated for 5 days by crystal violet staining based spectrophotometry Cytotoxicity against human D423 cells with ENO1 deficient assessed as reduction in cell viability incubated for 5 days by crystal violet staining based spectrophotometry	[PMID: 33130289]

Clinical Trial

Molecular Weight

195.11

Formula

C₅H₁₀NO₅P

CAS No.

2004714-32-1

Appearance

Viscous Liquid

Color

Brown to reddish brown

SMILES

OP(O)(C1C(N(O)CCC1)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (512.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.1253 mL	25.6266 mL	51.2531 mL
5 mM	1.0251 mL	5.1253 mL	10.2506 mL
10 mM	0.5125 mL	2.5627 mL	5.1253 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (10.66 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (10.66 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (10.66 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.96%

Select Batch:

Data Sheet (275 KB) SDS (252 KB)

COA (254 KB) HNMR (321 KB) LCMS (92 KB)

Handling Instructions (2659 KB)

References

[1]. BOARD OF REGENTS, et al. Enolase inhibitors and methods of treatment there with. WO2016145113A1

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.1253 mL	25.6266 mL	51.2531 mL	128.1328 mL
	5 mM	1.0251 mL	5.1253 mL	10.2506 mL	25.6266 mL
	10 mM	0.5125 mL	2.5627 mL	5.1253 mL	12.8133 mL
	15 mM	0.3417 mL	1.7084 mL	3.4169 mL	8.5422 mL
	20 mM	0.2563 mL	1.2813 mL	2.5627 mL	6.4066 mL
	25 mM	0.2050 mL	1.0251 mL	2.0501 mL	5.1253 mL
	30 mM	0.1708 mL	0.8542 mL	1.7084 mL	4.2711 mL
	40 mM	0.1281 mL	0.6407 mL	1.2813 mL	3.2033 mL
	50 mM	0.1025 mL	0.5125 mL	1.0251 mL	2.5627 mL
	60 mM	0.0854 mL	0.4271 mL	0.8542 mL	2.1355 mL
	80 mM	0.0641 mL	0.3203 mL	0.6407 mL	1.6017 mL
	100 mM	0.0513 mL	0.2563 mL	0.5125 mL	1.2813 mL

Hex Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hex2004714-32-1EnolasePhosphopyruvate hydrataseInhibitorinhibitorinhibit

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Hex

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Other Forms of Hex:

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Complete Stock Solution Preparation Table

Hex Related Classifications

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