2. Metabolic Enzyme/Protease
  3. HIF/HIF Prolyl-Hydroxylase
  4. HIF-1α-IN-4

HIF-1α-IN-4 is a HIF-1α inhibitor with an IC50 value of 24 nM (in HEK293T cell). HIF-1α-IN-4 downregulates VEGF and PDK1 mRNA expressions under hypoxia. HIF-1α-IN-4 can be used in the research of cancer.

For research use only. We do not sell to patients.

HIF-1α-IN-4 Chemical Structure

HIF-1α-IN-4 Chemical Structure

CAS No. : 2826221-23-0

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Based on 1 publication(s) in Google Scholar

HIF-1α-IN-4 Related Antibodies

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HIF-1α HIF

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Description

HIF-1α-IN-4 is a HIF-1α inhibitor with an IC50 value of 24 nM (in HEK293T cell). HIF-1α-IN-4 downregulates VEGF and PDK1 mRNA expressions under hypoxia. HIF-1α-IN-4 can be used in the research of cancer[1].

IC50 & Target

HIF-1α[1]
In Vitro

HIF-1α-IN-4 (compound 31, 24 h) shows inhibitory effects against HIF-1α transcription in HEK293T cells, with an IC50 value of 24 nM[1].
HIF-1α-IN-4 (0-50 μM, 24 h) downregulates VEGF and PDK1 mRNA expressions under hypoxia in HEK293T cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HIF-1α-IN-4 Related Antibodies

RT-PCR[1]

Cell Line: HEK293T cells under hypoxia
Concentration: 0-50 μM approximately
Incubation Time: 24 h
Result: Dose-dependently reduces VEGF and PDK1 mRNA expression.
Molecular Weight

280.28

Formula

C16H12N2O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=NOC2=CC=CC=C12)NC3=CC=C(C(C)=O)C=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 83.33 mg/mL (297.31 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5679 mL 17.8393 mL 35.6786 mL
5 mM 0.7136 mL 3.5679 mL 7.1357 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.5679 mL 17.8393 mL 35.6786 mL 89.1965 mL
5 mM 0.7136 mL 3.5679 mL 7.1357 mL 17.8393 mL
10 mM 0.3568 mL 1.7839 mL 3.5679 mL 8.9197 mL
15 mM 0.2379 mL 1.1893 mL 2.3786 mL 5.9464 mL
20 mM 0.1784 mL 0.8920 mL 1.7839 mL 4.4598 mL
25 mM 0.1427 mL 0.7136 mL 1.4271 mL 3.5679 mL
30 mM 0.1189 mL 0.5946 mL 1.1893 mL 2.9732 mL
40 mM 0.0892 mL 0.4460 mL 0.8920 mL 2.2299 mL
50 mM 0.0714 mL 0.3568 mL 0.7136 mL 1.7839 mL
60 mM 0.0595 mL 0.2973 mL 0.5946 mL 1.4866 mL
80 mM 0.0446 mL 0.2230 mL 0.4460 mL 1.1150 mL
100 mM 0.0357 mL 0.1784 mL 0.3568 mL 0.8920 mL
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HIF-1α-IN-4 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HIF-1α-IN-42826221-23-0HIF/HIF Prolyl-HydroxylaseHypoxia-inducible factorsHIFsHIF-PHTumorHEK293ThypoxiaVEGFPDK1Inhibitorinhibitorinhibit

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