2. Metabolic Enzyme/Protease Apoptosis Cell Cycle/DNA Damage
  3. E1/E2/E3 Enzyme Apoptosis MMP ClpP
  4. Hinokiflavone

Hinokiflavone is a novel modulator of pre-mRNA splicing activity extracted from plants with anti-inflammatory, anti-tumor and antiviral activities. Hinokiflavone is also a potent inhibitor for matrix metalloproteinases (MMPs). Hinokiflavone attenuates the virulence of Methicillin (HY-121544)-resistant staphylococcus aureus by inhibiting caseinolytic protease P (ClpP) with an IC50 value of 34.36 mg/mL. Hinokiflavone induces apoptosis via the reactive oxygen species-mitochondria-mediated apoptotic pathway and inhibits tumor cell migration and invasion. Hinokiflavone is a SUMO protease inhibitor against sentrin-specific protease 1 (SENP1) activity.

For research use only. We do not sell to patients.

Hinokiflavone Chemical Structure

Hinokiflavone Chemical Structure

CAS No. : 19202-36-9

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5 mg USD 140 In-stock
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Customer Review

Based on 2 publication(s) in Google Scholar

Hinokiflavone Related Antibodies

Top Publications Citing Use of Products

2 Publications Citing Use of MCE Hinokiflavone

View All MMP Isoform Specific Products:

View All Isoforms
MMP-1 MMP-2 MMP-3 MMP-8 MMP-9 MMP-13 MMP-14 MMP-17 ADAM10 ADAM17 MMP-7 MMP-12 MMP-10 MMP ADAM8

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Hinokiflavone is a novel modulator of pre-mRNA splicing activity extracted from plants with anti-inflammatory, anti-tumor and antiviral activities. Hinokiflavone is also a potent inhibitor for matrix metalloproteinases (MMPs). Hinokiflavone attenuates the virulence of Methicillin (HY-121544)-resistant staphylococcus aureus by inhibiting caseinolytic protease P (ClpP) with an IC50 value of 34.36 mg/mL. Hinokiflavone induces apoptosis via the reactive oxygen species-mitochondria-mediated apoptotic pathway and inhibits tumor cell migration and invasion. Hinokiflavone is a SUMO protease inhibitor against sentrin-specific protease 1 (SENP1) activity[1][2][3].

In Vitro

Hinokiflavone (500 μM, 90 min) inhibits splicing of the adenovirus and the HPV18E6 pre-mRNAs, showed a stronger effect on pre-mRNA splicing than Isoginkgetin (HY-N2117) in vitro[1].
Hinokiflavone (0-30 μM, 24 h) induces either cell cycle arrest or eventual cell death and increases levels of SUMOylated proteins by inhibiting sentrin-specific protease 1 (SENP1) activity in HEK 293 cells[1].
Hinokiflavone (0-40 μM, 24 h) suppresses colorectal tumor cell proliferation, cell migration and invasion ability but promotes cell Apoptosis via the mitochondria-mediated apoptotic pathway[2].
Hinokiflavone (0-128 μg/mL, 24 h) reduces the expression of virulence factors in staphylococcus aureus by directly interacting with caseinolytic protease P (ClpP)[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Hinokiflavone Related Antibodies

RT-PCR[1]

Cell Line: HEK293 cells
Concentration: 20-100 μM
Incubation Time: 24 h
Result: Induced changes in alternative splicing patterns and modulated splicing in HEK293 cells.

Cell Cycle Analysis[1]

Cell Line: HEK293 cells, HeLa, NB4 cells
Concentration: 0-30 μM
Incubation Time: 24 h
Result: Showed either cell cycle arrest or eventual cell death, dependent upon concentrations in HEK293 cells, HeLa, NB4 cells.

Apoptosis Analysis[2]

Cell Line: Colorectal tumor cells
Concentration: 0-40 μM
Incubation Time: 24 h
Result: Reduced and shrinked tumor cells with nuclear fragmentation and condensed nuclei formation, decreased the expression of Bcl-2, but increased the expression of Bax and cleaved caspase-3.

Western Blot Analysis[2]

Cell Line: Colorectal tumor cells
Concentration: 0-40 μM
Incubation Time: 24 h
Result: Inhibited the expression of MMP2 and MMP9 and increased the expression of TIMP2 in colorectal tumor cells.

Cell Cytotoxicity Assay[3]

Cell Line: HEK 293T cells
Concentration: 0-256 μg/mL
Incubation Time: 24 h
Result: Did not affect HEK293T cells viability.
In Vivo

Hinokiflavone (25 or 50 mg/kg, i.p., daily for 24 days) has antitumor activity via suppressing tumor proliferation, metastasis and inducing Apoptosis in CT26 tumor-bearing mice[2].
Hinokiflavone (100 mg/kg, s.c., every 12 h for 96 h) shows a protective effect on pneumonia infection by Improving survival rate in mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: CT26 tumor-bearing mice model[2]
Dosage: 25 or 50 mg/kg
Administration: i.p., daily for 24 days
Result: Resulted in retarded tumor progression and reduction of tumor weight without affecting body weight of mice, suppressed tumor proliferation and invasion ability by staining nuclear Ki-67 and MMP9, induced the apoptosis by staining cleaved caspase-3.
Molecular Weight

538.46

Formula

C30H18O10
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C=C(C2=CC=C(O)C=C2)OC3=CC(O)=C(OC4=CC=C(C5=CC(C6=C(O)C=C(O)C=C6O5)=O)C=C4)C(O)=C13
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (92.86 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 2 mg/mL (3.71 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8571 mL 9.2857 mL 18.5715 mL
5 mM 0.3714 mL 1.8571 mL 3.7143 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 0.83 mg/mL (1.54 mM); Clear solution

    This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  50% PEG300    50% Saline

    Solubility: 10 mg/mL (18.57 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.80%

COA (258 KB) HNMR (424 KB) RP-HPLC (124 KB) MS (227 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO 1 mM 1.8571 mL 9.2857 mL 18.5715 mL 46.4287 mL
DMSO 5 mM 0.3714 mL 1.8571 mL 3.7143 mL 9.2857 mL
10 mM 0.1857 mL 0.9286 mL 1.8571 mL 4.6429 mL
15 mM 0.1238 mL 0.6190 mL 1.2381 mL 3.0952 mL
20 mM 0.0929 mL 0.4643 mL 0.9286 mL 2.3214 mL
25 mM 0.0743 mL 0.3714 mL 0.7429 mL 1.8571 mL
30 mM 0.0619 mL 0.3095 mL 0.6190 mL 1.5476 mL
40 mM 0.0464 mL 0.2321 mL 0.4643 mL 1.1607 mL
50 mM 0.0371 mL 0.1857 mL 0.3714 mL 0.9286 mL
60 mM 0.0310 mL 0.1548 mL 0.3095 mL 0.7738 mL
80 mM 0.0232 mL 0.1161 mL 0.2321 mL 0.5804 mL
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Hinokiflavone Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hinokiflavone19202-36-9E1/E2/E3 EnzymeApoptosisMMPClpPE1 activating enzymeE2 conjugating enzymeE3 ligating enzymeUbiquitin activating enzymeUbiquitin conjugating enzymeUbiquitin ligaseMatrix metalloproteinasesCaseinolytic protease Ppre-mRNA splicinganti-inflammatoryanti-tumorantiviralMMPsSUMO proteaseInhibitorinhibitorinhibit

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