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  6. Histone H1-derived Peptide

Histone H1-derived Peptide is a phosphopeptide and the peptide substrates containes a sequence in accordance with the optimal recognition motif for CDK, can be used to detecting CDK1-cyclinB1 enzyme activity.

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Histone H1-derived Peptide Chemical Structure

Histone H1-derived Peptide Chemical Structure

CAS No. : 889112-06-5

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Based on 1 publication(s) in Google Scholar

Histone H1-derived Peptide Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

Histone H1-derived Peptide is a phosphopeptide and the peptide substrates containes a sequence in accordance with the optimal recognition motif for CDK, can be used to detecting CDK1-cyclinB1 enzyme activity[1][2].

In Vitro

Histone H1-derived Peptide for Detecting CDK1-cyclinB1 Enzyme Activity[1]
(1) Add the CDK1-cyclinB1 complex to the reaction system to reach a final concentration of 10 nM.
(2) Add the Histone H1-derived Peptide substrate to achieve a final concentration of 100 μM.
(3) Add 200 μM of ATP.
(4) Add 20 mM Tris buffer, 50 mM NaCl, 0.5 mM DTT, and 0.04% BSA, ensuring that all components are mixed uniformly in the appropriate volumetric proportions, and adjust the pH to 7.
(5) Add 2 mM of MgCl2.
(6) Perform continuous ATP-coupled assays, utilizing the phosphorylation reaction generated by ADP, converting phosphoenolpyruvate (PEP) and NADH back to ATP catalyzed by pyruvate kinase/lactate dehydrogenase (PK/LDH). Monitor the decrease in NADH fluorescence, with an excitation wavelength of 340 nm and an emission wavelength of 460 nm.
(7) Analyze the reaction time curve using linear regression to obtain the reaction rate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Histone H1-derived Peptide Related Antibodies
Molecular Weight

1252.51

Formula

C56H101N17O15
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Gly-Gly-Gly-Pro-Ala-Thr-Pro-Lys-Lys-Ala-Lys-Lys-Leu
Sequence Shortening

GGGPATPKKAKKL
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture and light, under nitrogen

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
Purity & Documentation
References
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Histone H1-derived Peptide Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Histone H1-derived Peptide889112-06-5OthersPeptideBinding affinityInter-domain dynamicsInhibitorinhibitorinhibit

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