2. Stem Cell/Wnt Apoptosis
  3. Wnt β-catenin Apoptosis
  4. HLY78

HLY78, a Lycorine (HY-N0288) derivative, is a potent activator of the Wnt/β-catenin signaling pathway. HLY78 targets the DIX domain of Axin and promotes the Axin-LRP6 (lipoprotein receptor-related protein 6) association, thus promoting LRP6 phosphorylation and Wnt signal transduction. HLY78 can be used for subarachnoid hemorrhage (SAH) research.

For research use only. We do not sell to patients.

HLY78 Chemical Structure

HLY78 Chemical Structure

CAS No. : 854847-61-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 110 In-stock
Solution
10 mM * 1 mL in DMSO USD 110 In-stock
Solid
1 mg USD 40 In-stock
5 mg USD 100 In-stock
10 mg USD 160 In-stock
25 mg USD 310 In-stock
50 mg USD 500 In-stock
100 mg USD 800 In-stock
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Customer Review

Based on 12 publication(s) in Google Scholar

HLY78 Related Antibodies

Top Publications Citing Use of Products

    HLY78 purchased from MedChemExpress. Usage Cited in: Cancer Gene Ther. 2022 Dec 9.  [Abstract]

    Both HLY78 and BRD5631 significantly abrogate the effect of miR-219-5p on SKOV3 and SKOV3/DDP cell apoptosis.

    HLY78 purchased from MedChemExpress. Usage Cited in: Cancer Gene Ther. 2022 Dec 9.  [Abstract]

    Both HLY78 and BRD5631 significantly abrogate the effects of miR-219-5p on SKOV3 and SKOV3/DDP cell invasion (fig E) and migration (fig F).

    HLY78 purchased from MedChemExpress. Usage Cited in: Neurosci Bull. 2020 Oct;36(10):1171-1181.  [Abstract]

    Effects of HLY78 on neuronal apoptosis at 24 h after SAH. Representative Western blot bands of Bcl-2, Bax, and cleaved caspase.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    HLY78, a Lycorine (HY-N0288) derivative, is a potent activator of the Wnt/β-catenin signaling pathway. HLY78 targets the DIX domain of Axin and promotes the Axin-LRP6 (lipoprotein receptor-related protein 6) association, thus promoting LRP6 phosphorylation and Wnt signal transduction. HLY78 can be used for subarachnoid hemorrhage (SAH) research[1][2][3].

    IC50 & Target

    Wnt/β-catenin[1]
    In Vitro

    HLY78 inhibits apoptosis in tumor cells and embryonic cells caused by carbon ion radiation through activation of the Wnt/β-catenin pathway[2].
    HLY78 (20 μM, 0-48 h) significantly increases the colony formation ability by 2.78-fold and 2.88-fold for HGC-27 and AGS cells compared with the controls[3].
    HLY78 (20 μM, 0-48 h) elevates the migration ability of HGC-27 and AGS cells[3].
    HLY78 significantly increases TNKS expression, which is ameliorated by Dihydroartemisinin (HY-N0176)[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    HLY78 Related Antibodies
    In Vivo

    HLY78 (0-1.8 mg/kg, Intranasal injection, once) attenuates neuronal apoptosis and improves neurological deficits through the LRP6/GSK3β/β-catenin signaling pathway after SAH (subarachnoid hemorrhage) in rats[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Adult male Sprague-Dawley rats (280-310 g, n=9/group, SAH model)[2]
    Dosage: 0, 0.2, 0.6, and 1.8 mg/kg
    Administration: Intranasal injection, once, at 1 h post-SAH (subarachnoid hemorrhage)
    Result: Significantly attenuated the short-term and long-term neurobehavioral deficits, as well as the neuronal apoptosis after SAH at 0.6 mg/kg. Successfully delivered into the brain via intranasal administration at 0.6 mg/kg and was sufficient to significantly increase the phosphorylation of LRP6. Reversed the changes of the Bcl-2, Bax, and cleaved caspase 3 levels.
    Molecular Weight

    267.32

    Formula

    C17H17NO2
    CAS No.
    Appearance

    Solid

    Color

    Off-white to light yellow

    SMILES

    CCC1=CC=CC2=C1N(CC3=CC4=C(C=C32)OCO4)C
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 26 mg/mL (97.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.7408 mL 18.7042 mL 37.4083 mL
    5 mM 0.7482 mL 3.7408 mL 7.4817 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (7.78 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (7.78 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 98.49%

    COA (248 KB) RP-HPLC (247 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.7408 mL 18.7042 mL 37.4083 mL 93.5209 mL
    5 mM 0.7482 mL 3.7408 mL 7.4817 mL 18.7042 mL
    10 mM 0.3741 mL 1.8704 mL 3.7408 mL 9.3521 mL
    15 mM 0.2494 mL 1.2469 mL 2.4939 mL 6.2347 mL
    20 mM 0.1870 mL 0.9352 mL 1.8704 mL 4.6760 mL
    25 mM 0.1496 mL 0.7482 mL 1.4963 mL 3.7408 mL
    30 mM 0.1247 mL 0.6235 mL 1.2469 mL 3.1174 mL
    40 mM 0.0935 mL 0.4676 mL 0.9352 mL 2.3380 mL
    50 mM 0.0748 mL 0.3741 mL 0.7482 mL 1.8704 mL
    60 mM 0.0623 mL 0.3117 mL 0.6235 mL 1.5587 mL
    80 mM 0.0468 mL 0.2338 mL 0.4676 mL 1.1690 mL
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    HLY78 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    HLY78854847-61-3HLY 78HLY-78Wntβ-cateninApoptosisBeta cateninsubarachnoid hemorrhageSAHanti-apoptoticNeuronal apoptosistumor cellsembryonic cellsLRP6β-CateninBlood-brain barriergastric cancerInhibitorinhibitorinhibit

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