HLY78

Name: HLY78
Brand: MedChemExpress
SKU: HY-122816
Rating: 5.0 (13 reviews)

Cat. No.: HY-122816 Purity: 98.49%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

HLY78, a Lycorine (HY-N0288) derivative, is a potent activator of the Wnt/β-catenin signaling pathway. HLY78 targets the DIX domain of Axin and promotes the Axin-LRP6 (lipoprotein receptor-related protein 6) association, thus promoting LRP6 phosphorylation and Wnt signal transduction. HLY78 can be used for subarachnoid hemorrhage (SAH) research.

For research use only. We do not sell to patients.

HLY78 Chemical Structure

CAS No. : 854847-61-3

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 110	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 110	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 100	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 160	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 310	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 500	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 800	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 13 publication(s) in Google Scholar

10 Publications Citing Use of MCE HLY78

: HLY78 purchased from MedChemExpress. Usage Cited in: Cancer Gene Ther. 2022 Dec 9. [Abstract]; Both HLY78 and BRD5631 significantly abrogate the effect of miR-219-5p on SKOV3 and SKOV3/DDP cell apoptosis.

: HLY78 purchased from MedChemExpress. Usage Cited in: Cancer Gene Ther. 2022 Dec 9. [Abstract]; Both HLY78 and BRD5631 significantly abrogate the effects of miR-219-5p on SKOV3 and SKOV3/DDP cell invasion (fig E) and migration (fig F).

: HLY78 purchased from MedChemExpress. Usage Cited in: Neurosci Bull. 2020 Oct;36(10):1171-1181. [Abstract]; Effects of HLY78 on neuronal apoptosis at 24 h after SAH. Representative Western blot bands of Bcl-2, Bax, and cleaved caspase.

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Description

IC₅₀ & Target

Wnt/β-catenin^[1]

In Vitro

HLY78 inhibits apoptosis in tumor cells and embryonic cells caused by carbon ion radiation through activation of the Wnt/β-catenin pathway^[2].
HLY78 (20 μM, 0-48 h) significantly increases the colony formation ability by 2.78-fold and 2.88-fold for HGC-27 and AGS cells compared with the controls^[3].
HLY78 (20 μM, 0-48 h) elevates the migration ability of HGC-27 and AGS cells^[3].
HLY78 significantly increases TNKS expression, which is ameliorated by Dihydroartemisinin (HY-N0176)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

HLY78 (0-1.8 mg/kg, Intranasal injection, once) attenuates neuronal apoptosis and improves neurological deficits through the LRP6/GSK3β/β-catenin signaling pathway after SAH (subarachnoid hemorrhage) in rats^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Adult male Sprague-Dawley rats (280-310 g, n=9/group, SAH model)^[2]
Dosage:	0, 0.2, 0.6, and 1.8 mg/kg
Administration:	Intranasal injection, once, at 1 h post-SAH (subarachnoid hemorrhage)
Result:	Significantly attenuated the short-term and long-term neurobehavioral deficits, as well as the neuronal apoptosis after SAH at 0.6 mg/kg. Successfully delivered into the brain via intranasal administration at 0.6 mg/kg and was sufficient to significantly increase the phosphorylation of LRP6. Reversed the changes of the Bcl-2, Bax, and cleaved caspase 3 levels.

Molecular Weight

267.32

Formula

C₁₇H₁₇NO₂

CAS No.

854847-61-3

Appearance

Solid

Color

Off-white to light yellow

SMILES

CCC1=CC=CC2=C1N(CC3=CC4=C(C=C32)OCO4)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 26 mg/mL (97.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.7408 mL	18.7042 mL	37.4083 mL
5 mM	0.7482 mL	3.7408 mL	7.4817 mL
10 mM	0.3741 mL	1.8704 mL	3.7408 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (7.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (7.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.49%

Select Batch:

Data Sheet (278 KB) SDS (398 KB)

COA (248 KB) RP-HPLC (247 KB)

Handling Instructions (2659 KB)

References

[1]. Wang S, et al. Small-molecule modulation of Wnt signaling via modulating the Axin-LRP5/6 interaction. Nat Chem Biol. 2013 Sep;9(9):579-85. [Content Brief]

[2]. Luo X, et al. HLY78 Attenuates Neuronal Apoptosis via the LRP6/GSK3β/β-Catenin Signaling Pathway After Subarachnoid Hemorrhage in Rats. Neurosci Bull. 2020 Oct;36(10):1171-1181. [Content Brief]

[3]. Ma Y, et al. Dihydroartemisinin suppresses proliferation, migration, the Wnt/β-catenin pathway and EMT via TNKS in gastric cancer. Oncol Lett. 2021 Oct;22(4):688. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.7408 mL	18.7042 mL	37.4083 mL	93.5209 mL
	5 mM	0.7482 mL	3.7408 mL	7.4817 mL	18.7042 mL
	10 mM	0.3741 mL	1.8704 mL	3.7408 mL	9.3521 mL
	15 mM	0.2494 mL	1.2469 mL	2.4939 mL	6.2347 mL
	20 mM	0.1870 mL	0.9352 mL	1.8704 mL	4.6760 mL
	25 mM	0.1496 mL	0.7482 mL	1.4963 mL	3.7408 mL
	30 mM	0.1247 mL	0.6235 mL	1.2469 mL	3.1174 mL
	40 mM	0.0935 mL	0.4676 mL	0.9352 mL	2.3380 mL
	50 mM	0.0748 mL	0.3741 mL	0.7482 mL	1.8704 mL
	60 mM	0.0623 mL	0.3117 mL	0.6235 mL	1.5587 mL
	80 mM	0.0468 mL	0.2338 mL	0.4676 mL	1.1690 mL

HLY78 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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HLY78

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HLY78 Related Antibodies

10 Publications Citing Use of MCE HLY78

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Complete Stock Solution Preparation Table

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