HODHBt (Synonyms: HOOBt)

Cat. No.: HY-Y0493 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

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HODHBt (HOOBt) inhibits STAT5-SUMO interaction by blocking SUMOylation of phosphorylated STAT5. HODHBt enhances the magnitude of IL-15 signaling and significantly increases the natural killer (NK) cell cytotoxicity phenotype and function and the generation of cytokine-induced memory-like (CIML) natural killer (NK) cells. HODHBt can be used for research of HIV-infection and cancer.

For research use only. We do not sell to patients.

HODHBt Chemical Structure

CAS No. : 28230-32-2

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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HIV HIV-1 HIV-2

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

STAT5

In Vitro

HODHBt (100 μM; 24 h, and 48 h respectively) increases IL-15-mediated STAT phosphorylation in NK cells and enhances the cytotoxic profile of NK cells^[1].
HODHBt (100 μM; 24 h) enhances IL-15-mediated (100 ng/mL) cytotoxicity of NK cells against HIV-infected cells (K562 cells)^[1].
HODHBt (100 μM; 24 h) enhances IL-15-mediated (100 ng/mL, 6 h) cytotoxicity of NK cells against cancer cell lines (A2780 and U877; OCILy1 and OCILy10)^[1].
HODHBt (100 μM; 7 d) results in enhanced memory response upon recall in generation of human CIML NK cells in vitro^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

163.13

Formula

C₇H₅N₃O₂

CAS No.

28230-32-2

Appearance

Solid

Color

White to off-white

SMILES

O=C1N(O)N=NC2=CC=CC=C12

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 500 mg/mL (3065.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.1301 mL	30.6504 mL	61.3008 mL
5 mM	1.2260 mL	6.1301 mL	12.2602 mL
10 mM	0.6130 mL	3.0650 mL	6.1301 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.93%

Select Batch:

Data Sheet (273 KB) SDS (771 KB)

COA (250 KB) HNMR (132 KB) RP-HPLC (192 KB)

Handling Instructions (2659 KB)

References

[1]. Macedo AB, et al. The HIV Latency Reversal Agent HODHBt Enhances NK Cell Effector and Memory-Like Functions by Increasing Interleukin-15-Mediated STAT Activation. J Virol. 2022 Aug 10;96(15):e0037222. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	6.1301 mL	30.6504 mL	61.3008 mL	153.2520 mL
	5 mM	1.2260 mL	6.1301 mL	12.2602 mL	30.6504 mL
	10 mM	0.6130 mL	3.0650 mL	6.1301 mL	15.3252 mL
	15 mM	0.4087 mL	2.0434 mL	4.0867 mL	10.2168 mL
	20 mM	0.3065 mL	1.5325 mL	3.0650 mL	7.6626 mL
	25 mM	0.2452 mL	1.2260 mL	2.4520 mL	6.1301 mL
	30 mM	0.2043 mL	1.0217 mL	2.0434 mL	5.1084 mL
	40 mM	0.1533 mL	0.7663 mL	1.5325 mL	3.8313 mL
	50 mM	0.1226 mL	0.6130 mL	1.2260 mL	3.0650 mL
	60 mM	0.1022 mL	0.5108 mL	1.0217 mL	2.5542 mL
	80 mM	0.0766 mL	0.3831 mL	0.7663 mL	1.9157 mL
	100 mM	0.0613 mL	0.3065 mL	0.6130 mL	1.5325 mL

HODHBt Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HODHBt28230-32-2HOOBtSTATHIVHuman immunodeficiency virusLRANK cellsSTAT signalingmemory-like NK cellsInhibitorinhibitorinhibit

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