Homatropine Bromide

Name: Homatropine Bromide
Brand: MedChemExpress
SKU: HY-B0547A
Rating: 4.5 (1 reviews)

Cat. No.: HY-B0547A Purity: 99.99%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Homatropine Bromide is an orally active muscarinic acetylcholine receptor antagonist and can be used as an anticholinergic agent.

For research use only. We do not sell to patients.

Homatropine Bromide Chemical Structure

CAS No. : 51-56-9

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 61	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 61	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
1 g	USD 66	In-stock	Estimated Time of Arrival: December 31
5 g	USD 119	In-stock	Estimated Time of Arrival: December 31
10 g		Get quote
50 g		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Homatropine Bromide:

Homatropine methylbromide In-stock
Homatropine Get quote
Homatropine hydrochloride Get quote
Homatropine Bromide (Standard) Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Homatropine Bromide is an orally active muscarinic acetylcholine receptor antagonist and can be used as an anticholinergic agent^[1].

In Vitro

Homatropine (20 μM) alone produces a dose ratio of 259 in atrium from guinea-pigs, and produces a dose ratio of only 95.0 when combined with Hexamethonium Bromide (HY-B0569) in atrium from guinea-pigs^[1].
Homatropine has affinities for muscarinic receptors in stomach (pA2 = 7.13) and for those in atria mediating force (pA2 = 7.21) and rate (pA2 = 7.07) responses^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Homatropine methylbromide (9 mm x 5 mm conical suppository) causes prompt blockade of the effects of vagal stimulation on pulse rate and of intravenous acetylcholine on blood pressure in rats^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male albino rats^[3]
Dosage:	9 mm x 5 mm conical suppository
Administration:	By suppository
Result:	Blocked cardiovascular responses to vagal stimulation and acetylcholine; 10-20 min after insertion of the suppository the effects of vagal stimulation over a range of 2-16 Hz, 5 V, on pulse rate was virtually abolished and remained unchanged at 45-60 min.

Clinical Trial

Molecular Weight

356.25

Formula

C₁₆H₂₂BrNO₃

CAS No.

51-56-9

Appearance

Solid

Color

White to off-white

SMILES

O=C(C(C1=CC=CC=C1)O)O[C@H]2C[C@@H](CC3)N(C)[C@@H]3C2.Br

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (280.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (280.70 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8070 mL	14.0351 mL	28.0702 mL
5 mM	0.5614 mL	2.8070 mL	5.6140 mL
10 mM	0.2807 mL	1.4035 mL	2.8070 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 50 mg/mL (140.35 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.99%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (250 KB) RP-HPLC (240 KB)

Handling Instructions (2659 KB)

References

[1]. Leung, E. and F. Mitchelson, Modification by hexamethonium of the muscarinic receptors blocking activity of pancuronium and homatropine in isolated tissues of the guinea-pig. Eur J Pharmacol, 1982. 80(1): p. 11-7. [Content Brief]

[2]. Gilani, S.A. and L.B. Cobbin, Interaction of himbacine with carbachol at muscarinic receptors of heart and smooth muscle. Arch Int Pharmacodyn Ther, 1987. 290(1): p. 46-53. [Content Brief]

[3]. Cramer, M.B., L.A. Cates, and D.E. Clarke, Rectal absorption of homatropine [14C]methylbromide in the rat. J Pharm Pharmacol, 1978. 30(5): p. 284-6. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	2.8070 mL	14.0351 mL	28.0702 mL	70.1754 mL
	5 mM	0.5614 mL	2.8070 mL	5.6140 mL	14.0351 mL
	10 mM	0.2807 mL	1.4035 mL	2.8070 mL	7.0175 mL
	15 mM	0.1871 mL	0.9357 mL	1.8713 mL	4.6784 mL
	20 mM	0.1404 mL	0.7018 mL	1.4035 mL	3.5088 mL
	25 mM	0.1123 mL	0.5614 mL	1.1228 mL	2.8070 mL
	30 mM	0.0936 mL	0.4678 mL	0.9357 mL	2.3392 mL
	40 mM	0.0702 mL	0.3509 mL	0.7018 mL	1.7544 mL
	50 mM	0.0561 mL	0.2807 mL	0.5614 mL	1.4035 mL
	60 mM	0.0468 mL	0.2339 mL	0.4678 mL	1.1696 mL
	80 mM	0.0351 mL	0.1754 mL	0.3509 mL	0.8772 mL
	100 mM	0.0281 mL	0.1404 mL	0.2807 mL	0.7018 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.