Idebenone

Name: Idebenone
Brand: MedChemExpress
SKU: HY-N0303
Rating: 5.0 (7 reviews)

Cat. No.: HY-N0303 Purity: 99.36%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Idebenone, a well-appreciated mitochondrial protectant, exhibits protective efficacy against neurotoxicity and can be used for the research of Alzheimer's disease, Huntington's disease. Idebenone (oxidised form) has a dose-dependent inhibitory effect on the enzymatic metabolism of arachidonic acid in astroglial homogenates (IC₅₀=16.65 μM). Idebenone, a coenzyme Q10 analog and an antioxidant, induces apoptotic cell death in the human dopaminergic neuroblastoma SHSY-5Y cells. Idebenone quickly crosses the blood-brain barrier.

For research use only. We do not sell to patients.

Idebenone Chemical Structure

CAS No. : 58186-27-9

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 61	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 61	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 35	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 99	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 132	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of Idebenone:

Idebenone (Standard) Get quote

6 Publications Citing Use of MCE Idebenone

: Idebenone purchased from MedChemExpress. Usage Cited in: Oncotarget. 2018 Jan 30;9(15):12137-12153. [Abstract]; Retinal protection of Rasagiline combined with Idebenone against retinal ischemia-reperfusion (RIR) injury. Western blot analysis for the expression level of brain-derived neurotrophic factor (BDNF) in the whole IR-injured retinas.

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

Idebenone, a well-appreciated mitochondrial protectant, exhibits protective efficacy against neurotoxicity and can be used for the research of Alzheimer's disease, Huntington's disease. Idebenone (oxidised form) has a dose-dependent inhibitory effect on the enzymatic metabolism of arachidonic acid in astroglial homogenates (IC₅₀=16.65 μM)^[1]. Idebenone, a coenzyme Q10 analog and an antioxidant, induces apoptotic cell death in the human dopaminergic neuroblastoma SHSY-5Y cells^[2]. Idebenone quickly crosses the blood-brain barrier.

Cellular Effect

Cell Line	Type	Value	Description	References
Fibroblast	EC₅₀	592 nM Compound: Idebenone	Cytoprotective activity against BSO-induced human FRDA primary patient fibroblasts compound pretreated 12 hrs prior to BSO-induction measured after 48 hrs by calcein-AM-based cell viability assay Cytoprotective activity against BSO-induced human FRDA primary patient fibroblasts compound pretreated 12 hrs prior to BSO-induction measured after 48 hrs by calcein-AM-based cell viability assay	[PMID: 22137789]
Fibroblast	GI₅₀	98 μM Compound: Idebenone	Growth inhibition of human FRDA primary patient fibroblasts after 48 hrs by calcein-AM-based cell viability assay Growth inhibition of human FRDA primary patient fibroblasts after 48 hrs by calcein-AM-based cell viability assay	[PMID: 22137789]
HepG2	EC₅₀	0.94 μM Compound: Idebenone	Suppression of rotenone-induced ATP depletion in human HepG2 cells incubated for 2 hrs by Celltiter-Glo assay Suppression of rotenone-induced ATP depletion in human HepG2 cells incubated for 2 hrs by Celltiter-Glo assay	[PMID: 33180495]
HepG2	IC₅₀	64.5 μM Compound: Idebenone	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 24 hrs by Celltiter-blue assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 24 hrs by Celltiter-blue assay	[PMID: 33180495]
HL-60	IC₅₀	> 200 μM Compound: 3	Antiproliferative activity against human mitochondrial gene-knockout HL60 cells after 48 hrs by MTT assay Antiproliferative activity against human mitochondrial gene-knockout HL60 cells after 48 hrs by MTT assay	[PMID: 23432053]
HL-60	IC₅₀	36 μM Compound: 3	Antiproliferative activity against human HL60 cells after 48 hrs by MTT assay Antiproliferative activity against human HL60 cells after 48 hrs by MTT assay	[PMID: 23432053]
HL-60	IC₅₀	70 μM Compound: 3	Inhibition of tPMET complex in human mitochondrial gene-knockout HL60 cells after 30 mins by WST-1/mPMS reduction assay Inhibition of tPMET complex in human mitochondrial gene-knockout HL60 cells after 30 mins by WST-1/mPMS reduction assay	[PMID: 23432053]
HL-60	IC₅₀	77 μM Compound: 3	Inhibition of tPMET complex in human HL60 cells after 30 mins by WST-1/mPMS reduction assay Inhibition of tPMET complex in human HL60 cells after 30 mins by WST-1/mPMS reduction assay	[PMID: 23432053]
SH-SY5Y	IC₅₀	15.9 μM Compound: Idebenone	Cytotoxicity against human SH-SY5Y cells assessed as reduction in cell viability incubated for 24 hrs by Celltiter-blue assay Cytotoxicity against human SH-SY5Y cells assessed as reduction in cell viability incubated for 24 hrs by Celltiter-blue assay	[PMID: 33180495]

In Vitro

Idebenone, a compound with protective efficacy against neurotoxicity both in in vitro and in in vivo models, exists in two different oxidative states: the ubiquinol-derivative (reduced idebenone) and the ubiquinone-derivative (oxidised idebenone)^[1].
Idebenone (oxidised form) preferentially inhibits cyclooxygenase vs. lipoxygenase metabolism (IC₅₀ ratio lipoxygenase/cyclooxygenase: 3.22)^[1].
Idebenone (oxidised form) behaves similarly as indomethacin and piroxicam—two typical anti-inflammatory agents^[1].
Idebenone (oxidised form) inhibits total arachidonic acid metabolism, cyclooxygenase metabolism, lipoxygenase metabolism, lipoxygenase/cyclooxygenase with IC₅₀s of 16.65±3.48, 14.44±2.99, 46.51±7.20, and 3.22 μM^[1].
Idebenone (1-10 μM; for 24-72 h) has no effect on the cell viability of SHSY-5Y cells^[2].
Idebenone (25 μM or higher concentrations; for 24-72 h) shows significant reduction in cell viability of SHSY-5Y cells^[2].
Idebenone (30 μM) induces up-regulation of BAX expression and the caspase-3 activity^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	The human dopaminergic neuroblastma cell line, SHSY-5Y cells
Concentration:	1, 3, 10, 15, 25, 30, and 90 μM
Incubation Time:	24, 48, and 72 hours
Result:	Had no apparent detrimental effects on cell viability as indicated by the absence of trypan blue-positive staining in the cells at concentrations of 1, 3, 10 μM. Exhibited some degree of trypan blue-positive staining at 15 μM. Showed extensive trypan blue-positive staining at 25 μM and 30 μM.

RT-PCR^[2]

Cell Line:	SHSY-5Y cells
Concentration:	10 μM, 30 μM
Incubation Time:	72 hours
Result:	The total RNA of BAX from SHSY-5Y cells exposed to 10 μM was not different from that of the untreated control cells. The BAX expression in SHSY-5Y cells exposed to 30 μM was significantly up-regulated when compared with the untreated control cells and cells exposed to 10 μM.

In Vivo

Idebenone (oxidised form) is a compound with protective efficacy against cerebrovascular disorders in vivo, including cerebral ischemia and hypertension-induced vascular lesions^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

338.44

Formula

C₁₉H₃₀O₅

CAS No.

58186-27-9

Appearance

Solid

Color

Yellow to orange

SMILES

O=C1C(CCCCCCCCCCO)=C(C)C(C(OC)=C1OC)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (295.47 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9547 mL	14.7737 mL	29.5473 mL
5 mM	0.5909 mL	2.9547 mL	5.9095 mL
10 mM	0.2955 mL	1.4774 mL	2.9547 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.39 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.39 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.39 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.36%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (241 KB) LCMS (90 KB)

Handling Instructions (2659 KB)

References

[1]. G Civenni, et al. Inhibitory effect of the neuroprotective agent idebenone on arachidonic acid metabolism in astrocytes.Eur J Pharmacol. 1999 Apr 9;370(2):161-7. [Content Brief]

[2]. Kwok-Keung Tai, et al. Idebenone induces apoptotic cell death in the human dopaminergic neuroblastoma SHSY-5Y cells. Neurotox Res. 2011 Nov;20(4):321-8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9547 mL	14.7737 mL	29.5473 mL	73.8683 mL
	5 mM	0.5909 mL	2.9547 mL	5.9095 mL	14.7737 mL
	10 mM	0.2955 mL	1.4774 mL	2.9547 mL	7.3868 mL
	15 mM	0.1970 mL	0.9849 mL	1.9698 mL	4.9246 mL
	20 mM	0.1477 mL	0.7387 mL	1.4774 mL	3.6934 mL
	25 mM	0.1182 mL	0.5909 mL	1.1819 mL	2.9547 mL
	30 mM	0.0985 mL	0.4925 mL	0.9849 mL	2.4623 mL
	40 mM	0.0739 mL	0.3693 mL	0.7387 mL	1.8467 mL
	50 mM	0.0591 mL	0.2955 mL	0.5909 mL	1.4774 mL
	60 mM	0.0492 mL	0.2462 mL	0.4925 mL	1.2311 mL
	80 mM	0.0369 mL	0.1847 mL	0.3693 mL	0.9234 mL
	100 mM	0.0295 mL	0.1477 mL	0.2955 mL	0.7387 mL

Idebenone Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Idebenone58186-27-9Mitochondrial MetabolismApoptosisAntioxidantOxidative,SHSY-5YprotectiveneurotoxicityAlzheimer'sHuntington'sarachidonicacidcoenzymeQ10dopaminergic neuroblastomaInhibitorinhibitorinhibit

Please select a local distributor ナミキ商事株式会社コスモ・バイオ株式会社
Product Name			Salutation
Applicant Name *			Email Address *
Phone Number *			Organization Name *
Department *			Requested quantity *
Country or Region *
Remarks

Product Name			Lot #
Applicant Name *			Email Address *
Phone Number			Department *
Organization Name *			Country or Region *
Remarks

Idebenone

Customer Review

Other Forms of Idebenone:

Idebenone Related Antibodies

6 Publications Citing Use of MCE Idebenone

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Idebenone Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Idebenone Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report