2. Protein Tyrosine Kinase/RTK
  3. Src
  4. iHCK-37

iHCK-37  (Synonyms: ASN05260065)

Cat. No.: HY-139147 Purity: 99.52%
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iHCK-37 (ASN05260065) is a potent and specific Hck inhibitor with a Ki value of 0.22 μM. iHCK-37 blocks HIV-1 viral replication with an EC50 value of 12.9 μM. iHCK-37 is used for chronic myeloid leukemia (CML) research.

For research use only. We do not sell to patients.

iHCK-37 Chemical Structure

iHCK-37 Chemical Structure

CAS No. : 516478-09-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO In-stock
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Based on 1 publication(s) in Google Scholar

iHCK-37 Related Antibodies

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Description

iHCK-37 (ASN05260065) is a potent and specific Hck inhibitor with a Ki value of 0.22 μM. iHCK-37 blocks HIV-1 viral replication with an EC50 value of 12.9 μM. iHCK-37 is used for chronic myeloid leukemia (CML) research[1].

IC50 & Target

Ki: 0.22 μM (Hck)[1]
In Vitro

iHCK-37 (5.0-20 μM; 24 hours) exhibits a potent in vitro antiproliferative activity. The dose (μM) for growth inhibition (GI50) is 5.0-5.8 μM for AML cell lines (HL60, KG1a and U937) and 9.1-19.2 μM for chronic myeloid leukemia cell lines (HEL and K562)[2].
iHCK-37 (3-9 μM; plus Erythropoietin) leads to a decrease in ERK, AKT and P70S6K phosphorylation of in lentivirus HCK silenced K562 and U937 cell lines[2].
iHCK-37 (3-9 μM) results in a decrease of p-HCK, p-ERK, p-AKT, p-70S6 in the cell line KG1a (AML/CD34+), in a dose-dependent manner[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

iHCK-37 Related Antibodies

Cell Viability Assay[1]

Cell Line: U937, HL60, KG1a, HEL and K562 cells
Concentration: 5.0-20 μM
Incubation Time: 24 hours
Result: Exhibited a reduction of growth in a dose-dependent manner.
Molecular Weight

544.73

Formula

C30H32N4O2S2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=CC=CC=C1N2CCOCC2)CSC3=C(C4=C(CCCC4)S5)C5=NC(CCC6=CC=CC=C6)=N3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 275 mg/mL (504.84 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8358 mL 9.1789 mL 18.3577 mL
5 mM 0.3672 mL 1.8358 mL 3.6715 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    10% Cremophor EL    80% (20% SBE-β-CD in Saline)

    Solubility: ≥ 0.5 mg/mL (0.92 mM); Clear solution

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8358 mL 9.1789 mL 18.3577 mL 45.8943 mL
5 mM 0.3672 mL 1.8358 mL 3.6715 mL 9.1789 mL
10 mM 0.1836 mL 0.9179 mL 1.8358 mL 4.5894 mL
15 mM 0.1224 mL 0.6119 mL 1.2238 mL 3.0596 mL
20 mM 0.0918 mL 0.4589 mL 0.9179 mL 2.2947 mL
25 mM 0.0734 mL 0.3672 mL 0.7343 mL 1.8358 mL
30 mM 0.0612 mL 0.3060 mL 0.6119 mL 1.5298 mL
40 mM 0.0459 mL 0.2295 mL 0.4589 mL 1.1474 mL
50 mM 0.0367 mL 0.1836 mL 0.3672 mL 0.9179 mL
60 mM 0.0306 mL 0.1530 mL 0.3060 mL 0.7649 mL
80 mM 0.0229 mL 0.1147 mL 0.2295 mL 0.5737 mL
100 mM 0.0184 mL 0.0918 mL 0.1836 mL 0.4589 mL
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iHCK-37 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

iHCK-37516478-09-4ASN05260065iHCK37iHCK 37ASN 05260065ASN-05260065SrcAntileukemiaAnti-HIVHckAntiviralSrc familyInhibitorinhibitorinhibit

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