JG26

Name: JG26
Brand: MedChemExpress
SKU: HY-120852
Rating: 4.5 (1 reviews)

Cat. No.: HY-120852 Purity: 98.01%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

JG26 is an ADAM17 inhibitor, with IC₅₀ values of 12 nM, 1.9 nM, 150 nM and 9.4 nM for ADAM8, ADAM17, ADAM10 and MMP-12, respectively.

For research use only. We do not sell to patients.

JG26 Chemical Structure

CAS No. : 1464910-32-4

Size	Price	Stock	Quantity
1 mg	USD 103	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 247	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 372	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 632	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 886	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1240	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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View All Isoforms

MMP-1 MMP-2 MMP-3 MMP-8 MMP-9 MMP-13 MMP-14 MMP-17 ADAM10 ADAM17 MMP-7 MMP-12 MMP-10 MMP ADAM8

Biological Activity
Purity & Documentation
References
Customer Review

Description

JG26 is an ADAM17 inhibitor, with IC₅₀ values of 12 nM, 1.9 nM, 150 nM and 9.4 nM for ADAM8, ADAM17, ADAM10 and MMP-12, respectively^[1].

IC₅₀ & Target^[1]

ADAM17

1.9 nM (IC₅₀)

MMP-12

9.4 nM (IC₅₀)

ADAM8

12 nM (IC₅₀)

ADAM10

150 nM (IC₅₀)

MMP-8

240 nM (IC₅₀)

MMP-9

1630 nM (IC₅₀)

MMP14

19500 nM (IC₅₀)

MMP-9

>500000 nM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	< 10 nM Compound: 21	Inhibition of ADAM-17 in human A2780 cells assessed as inhibition of pervanadate-induced sALCAM release incubated for 30 mins prior to pervanadate induction measured after 90 mins by ELISA Inhibition of ADAM-17 in human A2780 cells assessed as inhibition of pervanadate-induced sALCAM release incubated for 30 mins prior to pervanadate induction measured after 90 mins by ELISA	[PMID: 24044434]
A2780	IC₅₀	845 nM Compound: 21	Inhibition of ADAM-17 in human A2780 cells assessed as inhibition of EGF-induced sALCAM release incubated for 30 mins prior to EGF induction measured after 18 hrs by ELISA Inhibition of ADAM-17 in human A2780 cells assessed as inhibition of EGF-induced sALCAM release incubated for 30 mins prior to EGF induction measured after 18 hrs by ELISA	[PMID: 24044434]
SK-OV-3	IC₅₀	4272 nM Compound: 21	Inhibition of ADAM-17 in human SKOV3 cells transfected with luciferase gene assessed as inhibition of EGF-induced sALCAM release incubated for 30 mins prior to EGF induction measured after 18 hrs by ELISA Inhibition of ADAM-17 in human SKOV3 cells transfected with luciferase gene assessed as inhibition of EGF-induced sALCAM release incubated for 30 mins prior to EGF induction measured after 18 hrs by ELISA	[PMID: 24044434]
SK-OV-3	IC₅₀	58 nM Compound: 21	Inhibition of ADAM-17 in human SKOV3 cells transfected with luciferase gene assessed as inhibition of pervanadate-induced sALCAM release incubated for 30 mins prior to pervanadate induction measured after 90 mins by ELISA Inhibition of ADAM-17 in human SKOV3 cells transfected with luciferase gene assessed as inhibition of pervanadate-induced sALCAM release incubated for 30 mins prior to pervanadate induction measured after 90 mins by ELISA	[PMID: 24044434]

Molecular Weight

594.27

Formula

C₁₉H₂₂Br₂N₄O₆S

CAS No.

1464910-32-4

Appearance

Solid

Color

White to off-white

SMILES

O=C([C@@H](CCCNC(N)=O)NS(=O)(C1=CC=C(C=C1)OCC2=CC(Br)=CC(Br)=C2)=O)NO

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (168.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6827 mL	8.4137 mL	16.8274 mL
5 mM	0.3365 mL	1.6827 mL	3.3655 mL
10 mM	0.1683 mL	0.8414 mL	1.6827 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.01%

Select Batch:

Data Sheet (270 KB) SDS (251 KB)

COA (246 KB) HNMR (219 KB) RP-HPLC (467 KB) LCMS (103 KB)

Handling Instructions (2659 KB)

References

[1]. Doretta Cuffaro, et al. Discovery of Dimeric Arylsulfonamides as Potent ADAM8 Inhibitors. ACS Med Chem Lett. 2021 Oct 8;12(11):1787-1793. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.6827 mL	8.4137 mL	16.8274 mL	42.0684 mL
	5 mM	0.3365 mL	1.6827 mL	3.3655 mL	8.4137 mL
	10 mM	0.1683 mL	0.8414 mL	1.6827 mL	4.2068 mL
	15 mM	0.1122 mL	0.5609 mL	1.1218 mL	2.8046 mL
	20 mM	0.0841 mL	0.4207 mL	0.8414 mL	2.1034 mL
	25 mM	0.0673 mL	0.3365 mL	0.6731 mL	1.6827 mL
	30 mM	0.0561 mL	0.2805 mL	0.5609 mL	1.4023 mL
	40 mM	0.0421 mL	0.2103 mL	0.4207 mL	1.0517 mL
	50 mM	0.0337 mL	0.1683 mL	0.3365 mL	0.8414 mL
	60 mM	0.0280 mL	0.1402 mL	0.2805 mL	0.7011 mL
	80 mM	0.0210 mL	0.1052 mL	0.2103 mL	0.5259 mL
	100 mM	0.0168 mL	0.0841 mL	0.1683 mL	0.4207 mL

JG26 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

JG261464910-32-4JG 26JG-26MMPMatrix metalloproteinasesbreast cancerADAM17ADAM8Inhibitorinhibitorinhibit

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JG26

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JG26 Related Antibodies

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Complete Stock Solution Preparation Table

JG26 Related Classifications

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