2. Apoptosis
  3. Apoptosis
  4. Kaempferol 3-O-β-D-galactopyranoside

Kaempferol 3-O-β-D-galactopyranoside  (Synonyms: Trifolin)

Cat. No.: HY-N6605 Purity: 99.09%
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Kaempferol 3-O-β-D-galactopyranoside (Trifolin) is an orally active flavonoid derivative that can be found in the aerial parts of Consolida oliverana. Kaempferol 3-O-β-D-galactopyranoside exhibits antitumor and cardioprotective effects.

For research use only. We do not sell to patients.

Kaempferol 3-O-β-D-galactopyranoside Chemical Structure

Kaempferol 3-O-β-D-galactopyranoside Chemical Structure

CAS No. : 23627-87-4

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Based on 1 publication(s) in Google Scholar

Kaempferol 3-O-β-D-galactopyranoside Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Kaempferol 3-O-β-D-galactopyranoside

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Kaempferol 3-O-β-D-galactopyranoside (Trifolin) is an orally active flavonoid derivative that can be found in the aerial parts of Consolida oliverana. Kaempferol 3-O-β-D-galactopyranoside exhibits antitumor and cardioprotective effects[1][2][3].

Cellular Effect
Cell Line Type Value Description References
J774.2 IC50
161.32 μM
Compound: 3
Cytotoxicity against BALB/c mouse J774.2 cells after 72 hrs by trypan blue assay
Cytotoxicity against BALB/c mouse J774.2 cells after 72 hrs by trypan blue assay
[PMID: 19489596]
In Vitro

Kaempferol 3-O-β-D-galactopyranoside (12.5–50 μM, 24-48 h) inhibits the proliferation of NCI-H460 cells in a dose-dependent manner[2].
Kaempferol 3-O-β-D-galactopyranoside (12.5–50 μM, 48 h) increases the Sub-G1 phase cell population and induces apoptosis in NCI-H460 cells[2].
Kaempferol 3-O-β-D-galactopyranoside (12.5–50 μM, 48 h) modulates intrinsic and extrinsic apoptosis signaling pathways and induces apoptosis in NCI-H460 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Kaempferol 3-O-β-D-galactopyranoside Related Antibodies

Cell Viability Assay[2]

Cell Line: NCI-H460 human non-small cell lung cancer cells
Concentration: 12.5–50 μM
Incubation Time: 24 h and 48 h
Result: Decreased cell viability to less than 50% at 50 μM after 48 h;
Showed significant cytotoxicity only at 50 μM after 24 h.

Cell Cycle Analysis[2]

Cell Line: NCI-H460 human non-small cell lung cancer cells
Concentration: 12.5–50 μM
Incubation Time: 48 h
Result: Increased Sub-G1 phase cell population, decreased G1 phase cell population, no significant change in G2/M phase.

Western Blot Analysis[2]

Cell Line: NCI-H460 human non-small cell lung cancer cells
Concentration: 12.5–50 μM
Incubation Time: 24 h and 48 h
Result: Increased expression of Fas, FADD, and Bax;
Decreased expression of Bcl-2 and cIAP-1.
In Vivo

Kaempferol 3-O-β-D-galactopyranoside (0.1–10.0 mg/kg, p.o., once daily for 4 weeks) improves cardiac function and reduces cardiomyocyte apoptosis in Angiotensin II (AngII) (HY-13948)-induced hypertensive cardiac injury C57BL/6 mice model[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Angiotensin II (AngII) (HY-13948)-induced hypertensive cardiac injury C57BL/6 mice model[3]
Dosage: 0.1, 1.0, and 10.0 mg/kg
Administration: Oral gavage (p.o.), once daily for 4 weeks
Result: Improved left ventricular ejection fraction (LVEF) and fractional shortening (LVFS).
Alleviated inflammatory cell infiltration and pathological damage in cardiac tissue
. Reduced cardiomyocyte apoptosis and regulated Bax, Bcl-2, and cleaved caspase-3 protein expression
Molecular Weight

448.38

Formula

C21H20O11
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C1C2=C(O)C=C(O)C=C2OC(C3=CC=C(O)C=C3)=C1O[C@@H]4O[C@@H]([C@H](O)[C@H](O)[C@H]4O)CO
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (55.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2303 mL 11.1513 mL 22.3025 mL
5 mM 0.4461 mL 2.2303 mL 4.4605 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.25 mg/mL (2.79 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.25 mg/mL (2.79 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2303 mL 11.1513 mL 22.3025 mL 55.7563 mL
5 mM 0.4461 mL 2.2303 mL 4.4605 mL 11.1513 mL
10 mM 0.2230 mL 1.1151 mL 2.2303 mL 5.5756 mL
15 mM 0.1487 mL 0.7434 mL 1.4868 mL 3.7171 mL
20 mM 0.1115 mL 0.5576 mL 1.1151 mL 2.7878 mL
25 mM 0.0892 mL 0.4461 mL 0.8921 mL 2.2303 mL
30 mM 0.0743 mL 0.3717 mL 0.7434 mL 1.8585 mL
40 mM 0.0558 mL 0.2788 mL 0.5576 mL 1.3939 mL
50 mM 0.0446 mL 0.2230 mL 0.4461 mL 1.1151 mL
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Kaempferol 3-O-β-D-galactopyranoside Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Kaempferol 3-O-β-D-galactopyranoside23627-87-4TrifolinApoptosisHypertensive cardiac injury mice modelNCI-H460 cellsInhibitorinhibitorinhibit

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