2. Metabolic Enzyme/Protease Apoptosis NF-κB Immunology/Inflammation
  3. Endogenous Metabolite Apoptosis Reactive Oxygen Species
  4. L-Histidine hydrochloride hydrate

L-Histidine hydrochloride hydrate  (Synonyms: H-His-OH.HCl.H2O)

Cat. No.: HY-W014423 Purity: ≥98.0%
SDS
COA
Handling Instructions Technical Support

L-Histidine hydrochloride hydrate (H-His-OH.HCl.H2O) is an endogenous metabolite. L-Histidine hydrochloride hydrate scavenges hydroxyl radicals and singlet oxygen, regulate the absorption of zinc, copper and iron, exhibits anti-inflammatory and antioxidant activities. L-Histidine hydrochloride hydrate is blood brain barrier penetrable.

For research use only. We do not sell to patients.

L-Histidine hydrochloride hydrate Chemical Structure

L-Histidine hydrochloride hydrate Chemical Structure

CAS No. : 5934-29-2

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
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L-Histidine hydrochloride hydrate Related Antibodies

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Description

L-Histidine hydrochloride hydrate (H-His-OH.HCl.H2O) is an endogenous metabolite. L-Histidine hydrochloride hydrate scavenges hydroxyl radicals and singlet oxygen, regulate the absorption of zinc, copper and iron, exhibits anti-inflammatory and antioxidant activities. L-Histidine hydrochloride hydrate is blood brain barrier penetrable[1][2][3].

In Vitro

L-Histidine hydrochloride hydrate (1 mM, 20 h) enhances the cytotoxicity of H2O2, induces apoptosis by inducing DNA double-strand breaks[1].
L-Histidine hydrochloride hydrate (0.2-10 mM, 20 h) reduces zinc-induced GT1-7 cell death (ED50=0.2 mM), downregulates the expression of zinc-induced ER stress-related genes (such as GADD45, GADD34 and p8) and calcium homeostasis-related genes (such as Arc)[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

L-Histidine hydrochloride hydrate Related Antibodies

Apoptosis Analysis[1]

Cell Line: U937
Concentration: 1 mM
Incubation Time: 20 h
Result: Induced apoptosis.
In Vivo

L-Histidine hydrochloride hydrate (250 mg/kg, intra-rectal, 3 times a day for 5 days) exhibits anti-inflammatory efficacy in Acetic acid (HY-Y0319)-induced rats ulcerative colitis models[3].
L-Histidine hydrochloride hydrate (250 mg/kg, ip, single dose) exhibits neuroprotective efficacy in rat ischemia-reperfusion injury model[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Rats ulcerative colitis models[3]
Dosage: 250 mg/kg
Administration: intra-rectal administration, 3 times a day for 5 days
Result: Reduced the severity of colitis, reduced myeloperoxidase activity in colonic tissue.
Animal Model: Rat ischemia-reperfusion injury model[3]
Dosage: 250 mg/kg
Administration: ip, single dose
Result: Reduced ischemia-induced neuronal death.
Molecular Weight

209.63

Formula

C6H12ClN3O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(O)[C@](N)([H])CC1=CN=CN1.Cl.O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Store at room temperature, keep dry and cool

In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (477.02 mM; Need ultrasonic)

DMSO : 1 mg/mL (4.77 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.7702 mL 23.8512 mL 47.7024 mL
5 mM 0.9540 mL 4.7702 mL 9.5405 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (238.51 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

SDS (393 KB)

COA (252 KB) HNMR (113 KB) LCMS (228 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 4.7702 mL 23.8512 mL 47.7024 mL 119.2560 mL
H2O 5 mM 0.9540 mL 4.7702 mL 9.5405 mL 23.8512 mL
10 mM 0.4770 mL 2.3851 mL 4.7702 mL 11.9256 mL
15 mM 0.3180 mL 1.5901 mL 3.1802 mL 7.9504 mL
20 mM 0.2385 mL 1.1926 mL 2.3851 mL 5.9628 mL
25 mM 0.1908 mL 0.9540 mL 1.9081 mL 4.7702 mL
30 mM 0.1590 mL 0.7950 mL 1.5901 mL 3.9752 mL
40 mM 0.1193 mL 0.5963 mL 1.1926 mL 2.9814 mL
50 mM 0.0954 mL 0.4770 mL 0.9540 mL 2.3851 mL
60 mM 0.0795 mL 0.3975 mL 0.7950 mL 1.9876 mL
80 mM 0.0596 mL 0.2981 mL 0.5963 mL 1.4907 mL
100 mM 0.0477 mL 0.2385 mL 0.4770 mL 1.1926 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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L-Histidine hydrochloride hydrate Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L-Histidine hydrochloride5934-29-2H-His-OH.HCl.H2OEndogenous MetaboliteApoptosisReactive Oxygen Speciesantioxidantanti-inflammatoryulcerative colitisischemia-reperfusionInhibitorinhibitorinhibit

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